Human papillomavirus (HPV) type 16 is one of the major etiologic factors cervical cancer. Our study aims to investigate potentiality antiviral clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated Cas9 system (CRISPR/Cas9) targeting E6 and E7 oncogenes HPV16 as a potential chemosensitizer cisplatin (cis-diaminedichloroplatinum II; CDDP) for cancer.Specifically, therapeutic efficacy combination CDDP + E7-CRISPR/Cas9 was assessed in cancer cells xenograft...

Background/Aims: The Warburg effect is one of the main energy metabolism features supporting cancer cell growth. 20(S)-Rg3 exerts anti-tumor on ovarian partly by inhibiting effect. microRNAs are important regulators However, microRNA regulatory network mediating anti-Warburg was largely unknown. Methods: deep sequencing performed to identify 20(S)-Rg3-influenced in SKOV3 cells. miR-532-3p overexpressed mimic532-3p transfection and A2780 cells or inhibited inhibitor532-3p 20(S)-Rg3-treated...

Background: Many microRNAs (miRs) are dysregulated in cancers, and aberrant miR expression patterns have been suggested to correlate with chemo-resistance of cancer cells. We aim study the role miR-30 family members cisplatin-resistance ovarian Methods: qRT-PCR was used compare differential levels cell line A2780 its cisplatin-resistant derivative CP70. Changes cisplatin-sensitivity miR-30a-5p- miR-30c-5p-overexpressed-CP70 cells miR-30c-5p-inhibited-A2780 were examined by CCK8 assay...

The Warburg effect is one of the main metabolic features for cancers, with long non-coding RNA (lncRNA) being involved as a class crucial regulators. Our previous studies have shown that ginsenoside 20(S)-Rg3, an active saponin monomer extracted from red ginseng, inhibits in ovarian cancer cells. However, detailed lncRNA regulatory network modulated by 20(S)-Rg3 to prevent cells has not been explored.High-throughput sequencing was used screen out differentially expressed lncRNAs between...

Abnormal DNA methylation/demethylation is recognized as a hallmark of cancer. TET (ten-eleven translocation) family members are novel demethylation related proteins that dysregulate in multiple malignances. However, their effects on ovarian cancer remain to be elucidated. The changes during TGF-β1-induced epithelial-mesenchymal transition (EMT) SKOV3 and 3AO cells were detected. TET3 was ectopically expressed TGF-β1-treated examine its effect EMT phenotype. downstream target further...

Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) technology enables targeted gene editing, but cancer therapy with this approach requires improvements to enable safe and efficient delivery of CRISPR/Cas9 tumors. We developed evaluated a self-assembled liposome selectively deliver tissues. Our system effectively inhibited proliferation human papillomavirus (HPV) 16-positive cervical cells induced apoptosis by inactivating the HR-HPV16E6/E7...

Increasing evidence shows that human papillomavirus (HPV) E6/E7 deletion in cervical cancer cells may be related to the immunosuppressive tumor microenvironment and adverse reactions or resistance immune checkpoint blockade. Here, we demonstrate liposome delivery of CRISPR/cas9 can effectively knock out HPV, which, turn, induces autophagy triggers cell death-related activation by releasing damage-related molecular patterns. The results vivo experiments showed HPV-targeting guide...

The study of oceanic internal waves remains a critical area research within oceanography. With the rapid advancements in remote sensing and deep learning, it is now possible to extract valuable insights from vast datasets. In this context, by building datasets using learning models, we propose novel stripe segmentation algorithm for waves, leveraging synthetic aperture radar (SAR) images based on SegFormer architecture. Initially, hierarchical transformer encoder transforms image into...

Epithelial-mesenchymal transition (EMT) is one of the key mechanisms mediating cancer progression. MicroRNAs (miRs) are essential regulators gene expression by suppressing translation or causing degradation target mRNA. Growing evidence illustrates crucial roles miRs dysregulation in development and Here, we have found for first time that ginsenoside 20(S)-Rg3, a pharmacologically active component Panax ginseng, potently increases miR-145 downregulating methyltransferase DNMT3A to attenuate...

Abstract The development of chemo‐resistance impairs the outcome first line platinum‐based chemotherapies for ovarian cancer. Deregulation DNA methylation/demethylation provides a critical mechanism occurrence chemo‐resistance. ten‐eleven translocation (TET) family dioxygenases including TET1/2/3 plays an important part in demethylation, but their roles cisplatin resistance have not been elucidated. Using cisplatin‐sensitive and cisplatin‐resistant cancer cell models, we found that TET1 was...

A rhamnogalacturonan I domain, named RG-I-3A, was prepared from ginseng pectin by pectinase digestion and chromatography separation. Monosaccharide composition analysis revealed that it mainly composed of galacturonic acid, rhamnose, galactose, arabinose in a molar ratio 32.5:11.2:31.9:16.5, with molecular weight 50 kDa. Partial acid hydrolysis, monoclonal antibody detection, NMR spectra suggested RG-I-3A →4)-α-GalpA-(1→2)-α-Rhap-(1→disaccharide repeating units as backbone, β-1,4-galactan,...

Microbial transglutaminase (MTG) is an enzyme widely used in the food industry. Mutiple-site mutagenesis of Streptomyces mobaraensis was performed Escherichia coli. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibited highest activity 55.7 ± 1.4 U/mg longest half-life at 50 °C (418.2 min) 60 (24.8 min).

Epithelial-mesenchymal transition (EMT) is one of the key mechanisms mediating cancer progression. Snail1 has a pivotal role in regulation EMT, involving loss E-cadherin and concomitant upregulation vimentin, among other biomarkers. We have found FSCN1 promoted EMT ovarian cells, but precise mechanism process not been clearly elucidated.The levels snail1 were determined epithelial cancer(EOC) specimen cells by RT-qPCR. The changes makers effects on examined overexpression or depletion EOC...

Targeted therapy produces objective responses in bladder cancer patients, although the can be short. Meanwhile, response rates to immune are lower, but effects more durable. Based on these findings, it was hypothesized that urothelial carcinoma associated 1 (UCA1)-targeted could synergize with programmed cell death (PD-1) blockade enhance antitumor activity. To test this hypothesis, of CRISPR-Cas9 targeting UCA1 and PD-1 were assessed vitro vivo. It found gRNA/cas9-targeted induced apoptosis...

Abstract The Warburg effect, one of the metabolic hallmarks cancer, is responsible for rapid energy production through a high rate aerobic glycolysis. Ginsenoside 20(S)‐Rg3 antagonizes effect in ovarian cancer cells by upregulating some microRNAs, including miR‐519a‐5p, that target key enzymes involved How 20(S)‐Rg3‐upregulated miR‐519a‐5p influences remains poorly defined, however. Here we report while overexpression inhibited inhibition negated suppressive action against as evidenced...

Background: Ovarian cancer is a highly malignant gynecological cancer. Aerobic glycolysis one of the features cell metabolism. Studying molecular modulation Warburg effect in ovarian significantly valuable for understanding progression mechanism Materials and Methods: The expression level prognostic significance DNMT3A were analyzed using public databases. was overexpressed by plasmid transfection, interfered with specific siRNAs transfection. miR-603 mimic transfection or inhibited...

The National Medical Products Administration has authorized sodium oligomannate for treating mild-to-moderate Alzheimer's disease. In this study, an LC-MS/MS method was developed and validated to quantitate in different biomatrices. plasma pharmacokinetics, tissue distribution, excretion of Sprague-Dawley rats beagle dogs were systematically investigated. Despite its complicated structural composition, the absorption, metabolism, profiles oligosaccharides sizes terminal derivatives...

Objective: Adverse pregnancy outcomes are closely related to advanced maternal age (AMA; at ≥35 years). Little research has been reported on aneuploid abnormalities and pathogenic copy number variations (CNVs) affecting in women with AMA. The purpose of this study was assess CNVs associated AMA prenatal diagnosis determine the characteristics assist genetic counseling Methods: Among 277 fetuses AMA, 218 (78.7%) were isolated 59 (21.3%) non-isolated showed ultrasound anomalies from January...

Microbial transglutaminase (MTG) from Streptomyces mobaraensis is an important enzyme widely applied in food processing for the improvement of protein properties by catalyzing cross-linking proteins. In this work we aimed at improving production and enabling easy efficient purification process culture supernatants. Thus, recombinant vectors, with either a constitutive promoter (Pp5) or inducible (PnisA), controlling expression MTG gene fused to signal peptide Usp45 (SPusp45) were constructed...