A5102013843- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Ginseng Biological Effects and Applications
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer Cells and Metastasis
- Immune Response and Inflammation
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Antimicrobial Peptides and Activities
- CRISPR and Genetic Engineering
- Chemical Synthesis and Analysis
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Protease and Inhibitor Mechanisms
- Ubiquitin and proteasome pathways
- Cancer, Lipids, and Metabolism
- Pharmacological Effects of Natural Compounds
- Histone Deacetylase Inhibitors Research
- Neurological Disease Mechanisms and Treatments
- Peptidase Inhibition and Analysis
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
Zhejiang Yongning Pharma (China)
2024
First Affiliated Hospital of Xi'an Jiaotong University
2015-2024
The Seventh Affiliated Hospital of Sun Yat-sen University
2024
Sun Yat-sen University
2024
National University of Defense Technology
2024
Chengde Medical University
2024
Qingdao Agricultural University
2021-2023
Anhui University
2018-2019
Xi'an Jiaotong University
2007-2015
Center for Translational Molecular Medicine
2015
A non-protein-coding RNA, UCA1, has been cloned from human bladder TCC cell line BLZ-211 by using 5' and 3' RACE. The UCA1 full-length cDNA was 1442 bp. RT-PCR analysis indicated that is an embryonic development cancer-associated RNA. proliferative, migrative, invasive, drug resistance behaviors of BLS-211 were enhanced exogenous expression in vitro. Several potential target genes identified through microarray analysis. Moreover, the also increased tumorigenic cells nude mice. Results...
Human papillomavirus (HPV) type 16 is one of the major etiologic factors cervical cancer. Our study aims to investigate potentiality antiviral clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated Cas9 system (CRISPR/Cas9) targeting E6 and E7 oncogenes HPV16 as a potential chemosensitizer cisplatin (cis-diaminedichloroplatinum II; CDDP) for cancer.Specifically, therapeutic efficacy combination CDDP + E7-CRISPR/Cas9 was assessed in cancer cells xenograft...
Background/Aims: The Warburg effect is one of the main energy metabolism features supporting cancer cell growth. 20(S)-Rg3 exerts anti-tumor on ovarian partly by inhibiting effect. microRNAs are important regulators However, microRNA regulatory network mediating anti-Warburg was largely unknown. Methods: deep sequencing performed to identify 20(S)-Rg3-influenced in SKOV3 cells. miR-532-3p overexpressed mimic532-3p transfection and A2780 cells or inhibited inhibitor532-3p 20(S)-Rg3-treated...
Background: Many microRNAs (miRs) are dysregulated in cancers, and aberrant miR expression patterns have been suggested to correlate with chemo-resistance of cancer cells. We aim study the role miR-30 family members cisplatin-resistance ovarian Methods: qRT-PCR was used compare differential levels cell line A2780 its cisplatin-resistant derivative CP70. Changes cisplatin-sensitivity miR-30a-5p- miR-30c-5p-overexpressed-CP70 cells miR-30c-5p-inhibited-A2780 were examined by CCK8 assay...
The Warburg effect is one of the main metabolic features for cancers, with long non-coding RNA (lncRNA) being involved as a class crucial regulators. Our previous studies have shown that ginsenoside 20(S)-Rg3, an active saponin monomer extracted from red ginseng, inhibits in ovarian cancer cells. However, detailed lncRNA regulatory network modulated by 20(S)-Rg3 to prevent cells has not been explored.High-throughput sequencing was used screen out differentially expressed lncRNAs between...
The prognosis of patients with ovarian cancer has remained poor mainly because aggressive progression. Since epithelial-mesenchymal transition (EMT) is an important mechanism mediating invasion and metastasis cells, targeting the EMT process more efficacious less toxic compounds to inhibit great therapeutic value for treatment cancer. We have found first time that ginsenoside 20(S)-Rg3, a pharmacologically active component traditional Chinese herb Panax ginseng, potently blocks...
Cancer cells prefer to metabolize glucose through aerobic glycolysis, known as the Warburg effect. It plays a crucial role in proliferation and progression of cancer cells. However, complete mechanism remains elusive. In recent studies, signal transducer activator transcription 3 (STAT3) signaling has been discovered have roles cancer‑associated changes metabolism. this study, we find that ginsenoside 20(S)‑Rg3, pharmacologically active component traditional Chinese herb Panax ginseng,...
Drug resistance remains a major challenge in cancer therapy. Butein, polyphenolic compound, has been shown to exhibit anticancer activity through the inhibition of activation protein kinase B (PKB/AKT) and mitogen-activated (MAPK) pathways, which are two pathways known be involved cisplatin. Hence, we hypotheiszed that butein may chemosensitizer In present study, demonstrated synergistically enhanced growth inhibitory apoptosis-inducing effects cisplatin on HeLa cells. Moreover, combination...
MicroRNAs (miRs) are essential regulators of gene expression by suppressing translation or causing degradation target mRNA. Growing evidence sheds light on the crucial roles miR dysregulation in cancer development and progression. In this study, we focused role miR-30d transforming growth factor β1 (TGF-β1)-initiated epithelial-mesenchymal transition (EMT) ovarian cells.Transforming (10 ng/mL) was used to initiate EMT SKOV3 3AO cells. The miR-30 family members determined quantitative...
Abstract Hypoxia-mediated chemoresistance plays a crucial role in the development of ovarian cancer (OC). However, roles hypoxia-related genes (HRGs) and prognosis prediction theirs underlying mechanisms remain to be further elucidated. We intended identify validate classifiers hub HRGs for chemoresistance, diagnosis, as well immune microenvironment OC, explore function most HRG malignant phenotypes. The RNA expression clinical data were systematically evaluated OC training group. Univariate...
Background: Transforming growth factor beta 1 (TGFβ1) can induce epithelial-mesenchymal transition (EMT) in various human cancers, but the complex mechanisms underlying this have not been fully elucidated. Inhibitor of DNA binding (Id-1) has identified as a novel marker ovarian cancer progression. This study aims to investigate role Id-1 TGFβ1-induced EMT cells. Methods: Ovarian cells expressing or were incubated with TGFβ1. Changes markers E-cadherin, vimentin, N-cadherin, Id-1, and miR-29b...
Epithelial-mesenchymal transition (EMT) is one of the key mechanisms mediating cancer progression. MicroRNAs (miRs) are essential regulators gene expression by suppressing translation or causing degradation target mRNA. Growing evidence illustrates crucial roles miRs dysregulation in development and Here, we have found for first time that ginsenoside 20(S)-Rg3, a pharmacologically active component Panax ginseng, potently increases miR-145 downregulating methyltransferase DNMT3A to attenuate...
Endometrial cancer is one of the most common types gynecological malignancy worldwide. Novel biomarkers and therapeutic targets are imperative for improving patients' survival. Previous studies have suggested long non-coding RNA H19 as a potential biomarker. To investigate role in endometrial cancer, present study examined expression pattern tissues by quantitative polymerase chain reaction, characterized its function cell line via knocking down with small interfering RNAs. It was found that...
Targeted therapy produces objective responses in bladder cancer patients, although the can be short. Meanwhile, response rates to immune are lower, but effects more durable. Based on these findings, it was hypothesized that urothelial carcinoma associated 1 (UCA1)-targeted could synergize with programmed cell death (PD-1) blockade enhance antitumor activity. To test this hypothesis, of CRISPR-Cas9 targeting UCA1 and PD-1 were assessed vitro vivo. It found gRNA/cas9-targeted induced apoptosis...
Background: Ovarian cancer is a highly malignant gynecological cancer. Aerobic glycolysis one of the features cell metabolism. Studying molecular modulation Warburg effect in ovarian significantly valuable for understanding progression mechanism Materials and Methods: The expression level prognostic significance DNMT3A were analyzed using public databases. was overexpressed by plasmid transfection, interfered with specific siRNAs transfection. miR-603 mimic transfection or inhibited...