A5103286400- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- CRISPR and Genetic Engineering
- Ginseng Biological Effects and Applications
- Thyroid Disorders and Treatments
- Immune Response and Inflammation
- Vitamin K Research Studies
- Estrogen and related hormone effects
- Bone Metabolism and Diseases
- BRCA gene mutations in cancer
- Viral Infections and Vectors
- Virus-based gene therapy research
- Cervical Cancer and HPV Research
- Galectins and Cancer Biology
- RNA Research and Splicing
- Trace Elements in Health
- Inflammasome and immune disorders
- Ziziphus Jujuba Studies and Applications
Southwest Medical University
2024
First Affiliated Hospital of Xi'an Jiaotong University
2013-2022
First Hospital of China Medical University
2022
First Affiliated Hospital of Fujian Medical University
2022
Fujian Medical University
2022
Nanjing University of Aeronautics and Astronautics
2022
Zhejiang Chinese Medical University
2021
Soochow University
2019
Southern Medical University
2018
Nanfang Hospital
2018
Exosomes (Exo) secreted from hypoxia-conditioned bone marrow mesenchymal stem cells (BM-MSCs) were found to be protective for ischemic disease.However, the role of exosomal miRNA in effect BM-MSCs-derived Exo (Hypo-Exo) remains largely uncharacterized and poor specificity tissue targeting limits their clinical applications.Therefore, objective this study was examine Hypo-Exo on repair myocardium its underlying mechanisms.We further developed modified with high evaluate therapeutic...
To overcome the hostile hypoxic microenvironment of solid tumors, tumor cells secrete a large number non-coding RNA-containing exosomes that facilitate development and metastasis. However, precise mechanisms cell-derived during hypoxia are unknown. Here, we aim to clarify whether affects growth progression by transferring long RNA-urothelial cancer-associated 1 (lncRNA-UCA1) enriched secreted from bladder cancer cells. We used 5637 with high expression lncRNA-UCA1 as exosome-generating UMUC2...
Our previous studies identified UCA1 as a novel biomarker for bladder cancer and detected three variant transcripts of in the human TCC cell line BLZ-211 using northern blot analysis. One (1.4 kb) transcripts has been shown to play pivotal role progression embryonic development. In this study, we cloned second transcript (2.2 kb), designated UCA1a, which was identical previously reported upregulated drug resistant gene (CUDR). Sequence comparison kb transcript) UCA1a(CUDR) cDNA revealed...
Urothelial carcinoma associated 1 (UCA1) has been identified as an oncogenic long noncoding RNA (lncRNA) that is involved in bladder cancer progression and acts a diagnostic biomarker for carcinoma. Here, we studied the expression function of lncRNA-UCA1 hypoxic microenvironment cancer. The transcriptional activity were measured by quantitative real-time polymerase chain reaction luciferase assays. Cell proliferation apoptosis evaluated MTT assays flow cytometry. migration invasion detected...
This study aims to identify whether Urothelial Cancer Associated 1 (UCA1) regulates mitochondrial metabolic reprogramming in bladder cancer, and explore how UCA1 participates metabolism by the UCA1/miR-195/ARL2 signaling pathway; these findings may be aid development of tumor diagnostic therapeutic strategies.Bladder tissues were obtained from patients. Stable cell lines constructed, with ectopic expression UMUC2 cells knockdown 5637 cells. The levels UCA1, miR-195, ARL2 detected real-time...
Human papillomavirus (HPV) type 16 is one of the major etiologic factors cervical cancer. Our study aims to investigate potentiality antiviral clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated Cas9 system (CRISPR/Cas9) targeting E6 and E7 oncogenes HPV16 as a potential chemosensitizer cisplatin (cis-diaminedichloroplatinum II; CDDP) for cancer.Specifically, therapeutic efficacy combination CDDP + E7-CRISPR/Cas9 was assessed in cancer cells xenograft...
Exosomes are typically involved in cellular communication and signaling. Macrophages play a key role lipopolysaccharide (LPS)-induced sepsis. However, the molecular comparison of exosomes derived from LPS-induced macrophage has not been well analyzed. The macrophage-exosomes validated protein composition those investigated by isobaric tags for relative absolute quantification (iTRAQ) mass spectrometry. A total 5056 proteins identified macrophage-exosomes. We discovered 341 increased 363...
Long non-coding RNA urothelial carcinoma associated 1 (lncRNA-UCA1) is upregulated in bladder cancer and plays a pivotal role progression metastasis. Recent studies our research found that lncRNA-UCA1 may be an important biomarker therapeutic target for cancer. However, the molecular mechanism involved upregulation of largely unknown. In present study, we showed expression cells was by transcription factor CCAAT/enhancer binding protein α (C/EBPα), which only candidate simultaneously...
Epithelial-mesenchymal transition (EMT) is one of the key mechanisms mediating cancer progression. MicroRNAs (miRs) are essential regulators gene expression by suppressing translation or causing degradation target mRNA. Growing evidence illustrates crucial roles miRs dysregulation in development and Here, we have found for first time that ginsenoside 20(S)-Rg3, a pharmacologically active component Panax ginseng, potently increases miR-145 downregulating methyltransferase DNMT3A to attenuate...
Objective Matrix Gla protein (MGP) is a potent calcification inhibitor. Mgp-/- mice display increased proportion of brown adipose tissue. However, whether MGP involved in fat metabolism remains unclear. This study aims to investigate the involvement. Methods Expression adipocyte differentiation markers was examined by RT-qPCR. Adipocyte formation assessed Oil Red staining. Serum triglyceride, cholesterol, and desphosphorylated-uncarboxylated (dp-ucMGP) were quantified ELISA. Visceral...
Metastasis frequently occurs in advanced ovarian cancer, which not only leads to substantial mortality but also becomes a major challenge effective treatment. Epithelial-mesenchymal transition (EMT) is key mechanism facilitating cancer metastasis. Targeting the EMT process with more efficacious and less toxic agents prevent metastasis of significant therapeutic value for The anti-EMT function ginsenoside Rb1, monomer composition extracted from traditional Chinese herb Panax ginseng or P....
Osteoporosis is a disease characterized by abnormal skeletal fragility due to an imbalance in bone homeostasis. Finding therapeutic targets that promote formation valuable for treating osteoporosis. Discoidin domain receptor 2 (DDR2) tyrosine kinase promotes the differentiation of bone-depositing cells, osteoblasts, and formation. We investigated role DDR2 osteoclastogenesis, cells responsible resorption. found that, although differentiated osteoclasts had catalytically active DDR2, its...
Matrix Gla protein (MGP) is an extracellular responsible for inhibiting mineralization. MGP inhibits osteoblast mineralization and bone formation by regulating the deposition of matrix. However, Mgp-/- mice display osteopenic phenotype. To explain this contradiction, we investigated role in osteoclastogenesis, other side remodeling. We found that expression markedly increased osteoclastic commitment. Osteoclast differentiation resorption are accelerated depletion while suppressed...
Rab27A is a peculiar member in Rab family and has been suggested to play essential roles the development of human cancers. However, association between expression clinicopathological characteristics colorectal cancer (CRC) not elucidated yet.One-step quantitative real-time polymerase chain reaction (qPCR) test with 18 fresh-frozen CRC samples immunohistochemistry (IHC) analysis 112 cases were executed evaluate relationship features CRC. Cox regression Kaplan-Meier survival analyses performed...
Abstract Emerging evidence suggests that miR‐143 plays an important role in the regulation of tumor sensitivity to chemotherapeutic agents. The study explores underlying mechanism reversing cisplatin resistance ovarian cancer. cisplatin‐resistant cancer cell line A2780/CDDP was induced and established via treating A2780 cells by gradually increasing concentrations. IC 50 values were 218.10 ± 1.12 21.99 μM, respectively. Quantitative real‐time polymerase chain reaction (qRT‐PCR) results...
We selected 13 tag single nucleotide polymorphisms (tSNPs) to investigate whether they were associated with breast cancer risk in the Chinese Han population. Upon statistical analyses of clinical data from 551 patients and 577 controls, we found that six SNPs cancer; namely, rs4973768(Odds ratio (OR) = 1.30, 95% confidence interval (CI) =1.01-1.67), rs981782(OR =1.30, CI=1.01-1.66), rs1432679(OR =0.84, CI=0.70-0.99), rs10759243(OR=1.30, 95%CI=1.09-1.55), rs10822013(OR =1.18, CI=1.00-1.39)...