Exosomes (Exo) secreted from hypoxia-conditioned bone marrow mesenchymal stem cells (BM-MSCs) were found to be protective for ischemic disease.However, the role of exosomal miRNA in effect BM-MSCs-derived Exo (Hypo-Exo) remains largely uncharacterized and poor specificity tissue targeting limits their clinical applications.Therefore, objective this study was examine Hypo-Exo on repair myocardium its underlying mechanisms.We further developed modified with high evaluate therapeutic...

Proangiogenesis is generally regarded as an effective approach for treating ischemic heart disease. Vascular endothelial growth factor (VEGF)-A a strong and essential proangiogenic factor. Reactive oxygen species (ROS), endoplasmic reticulum (ER) stress, autophagy are implicated in the process of angiogenesis. This study designed to clarify regulatory mechanisms underlying VEGF-A, ROS, ER autophagy, angiogenesis acute myocardial infarction (AMI). A mouse model AMI was successfully...

AimsCardiomyocyte apoptosis contributes to the development of diabetic cardiomyopathy. How elevated glucose levels associated with diabetes cause cell death is unknown. Here we report that high glucose-induced cardiomyocyte mediated via monocyte chemotactic protein-1 (MCP-1) production and induction a novel zinc-finger protein.

Osteoclasts (OCs) are responsible for bone resorption in inflammatory joint diseases. Monocyte chemotactic protein-1 (MCP-1) has been shown to induce differentiation of monocytes OC precursors, but nothing is known about the underlying mechanisms. Here, we elucidate how MCPIP, induced by MCP-1, mediates this differentiation. Knockdown MCPIP abolished MCP-1-mediated expression markers, tartrate-resistant acid phosphatase, and serine protease cathepsin K. Expression p47PHOX its membrane...

Resveratrol, a polyphenol compound derived from various edible plants, protects against sepsis-induced acute kidney injury (AKI) via its anti-inflammatory activity, but the underlying mechanisms remain largely unknown. In this study, rat model of sepsis was established by cecal ligation and puncture (CLP), 30 mg/kg resveratrol intraperitoneally administrated immediately after CLP operation. HK-2 cells treated 1 μg/ml lipopolysaccharide, 0.2 μM tunicamycin, 2.5 mM irestatin 9389 20 were used...

As an important transcription factor, heat shock factor 1 (HSF1) plays endogenous anti-inflammation role in the body and can alleviate multiple organ dysfunction caused by sepsis, which contributes to uncontrolled inflammatory response. The NLRP3 inflammasome is a supramolecular complex that key roles immune surveillance. Inflammation accomplished activation, leads proteolytic maturation of IL-1β pyroptosis. However, whether HSF1 involved activation septic acute lung injury (ALI) has not...

Acute necrotizing pancreatitis (ANP) is a common severe critical illness with high mortality rate. Resveratrol, polyphenol compound derived from various plants such as grape skin, peanut, berry and veratrum, exhibits multiple biological activities, especially potent anti‑inflammatory activity, but its effect on ANP has not yet been fully elucidated. The present study aimed to investigate the effects of resveratrol L-arginine-induced possible mechanisms. A mouse model was established by 2...

An ultrasensitive aptasensor for the detection of Mucin 1 (MUC1) protein based on fluorescence resonance energy transfer (FRET) between carbon dots (CDs) and graphene oxide (GO) is reported herein.

Hydroxysafflor Yellow A (HSYA), a most representative ingredient of Carthamus tinctorius L., had long been used in treating ischaemic cardiovascular diseases China and exhibited prominently anticoagulant pro-angiogenic activities, but the underlying mechanisms remained largely unknown. This study aimed to further elucidate effect mechanism HSYA on cardiac dysfunction. C57 mouse model acute myocardial infarction (AMI) was firstly established, 25 mg/kg intraperitoneally injected immediately...

Sepsis, a systemic inflammatory response to infection, is the major cause of death in intensive care units (ICUs). The mortality rate sepsis remains high even though treatment and understanding both continue improve. Sinomenine (SIN) natural alkaloid extracted from Chinese medicinal plant Sinomenium acutum, its hydrochloride salt (Sinomenine hydrochloride, SIN-HCl) widely used treat rheumatoid arthritis (RA). However, role unclear. In present study, we investigated SIN-HCl induced by cecal...

Fibrotic animal models are critical for the pathogenesis investigations and drug explorations in systemic sclerosis (SSc). The bleomycin (BLM)-induced mouse model is classical most widely used fibrosis model. However, traditional subcutaneous injection of BLM rarely induced diffuse skin lung lesions. Hypochlorous acid (HOCl)-induced mice a more representative that have cutaneous lesions, renal involvement. fibrotic immunological features this not fully elucidated. Here, we injected BALB/c...

Objective— To identify circulating microRNAs that are differentially expressed in severe coronary heart disease with well or poorly developed collateral arteries and to investigate their mechanisms of action vivo vitro. Approach Results— In our study, we identified a microRNA, miR-15b-5p, low expression that, nevertheless, characterized patients sufficient artery function. Moreover, murine hindlimb ischemia model, situ hybridization miR-15b-5p was specifically vascular endothelial cells...

This study was undertaken to explore the value of interleukin-1 receptor 2 (IL1R2) as a novel potential biomarker for diagnosis sepsis and discrimination gram-negative (G)/gram-positive (G) bacterial sepsis. The performed in Kunming mice septic patients. Inactive Escherichia coli or Staphylococcus aureus were used stimulate (10 CFU/kg). In clinical study, patients with different pathogen infection studied, healthy volunteers systemic inflammatory response syndrome without definite enrolled...

Doxorubicin (Dox) is known to cause cardiomyopathy and congestive heart failure upon chronic administration. The mechanisms underlying these toxicities remain uncertain but have been attributed, at least in part, by induction of cardiac cell apoptosis. Fas ligation with its cognate ligand (FasL) induces apoptosis activates cellular inflammatory responses associated tissue injury. We determined whether interruption Fas/FasL interaction cardiac-targeted expression soluble (sFas), a competitive...

Monocytic cells enhance neovascularization by releasing proangiogenic mediators and/or transdifferentiating into endothelial-like cells. However, the mechanisms that govern this transdifferentiation process are largely unknown. Recently, monocyte chemotactic protein-1 (MCP-1)-induced protein (MCPIP) has been identified as a novel CCCH-type zinc-finger expressed primarily in monocytic Here, we analyzed whether MCPIP might exert angiogenic effects promoting differentiation of endothelial cell...

Zinc finger protein 667 (ZNF667, also referred as Mipu1), a widely expressed KRAB/C2H2‑type zinc transcription factor, can protect against hypoxic‑ischemic myocardial injury. Pro‑angiogenesis is regarded promising strategy for the treatment of acute infarction (AMI). However, whether ZNF667 involved in angiogenesis following AMI remains to be elucidated. The present study reported that expression CD31‑positive endothelial cells (ECs) was upregulated heart mice. Hypoxic challenge (1% oxygen)...

Endostatin is an endogenous inhibitor of vascular endothelium. It can inhibit endothelial cell migration, proliferation, and angiogenesis mainly used for anticancer therapy. We have previously found that endostatin important node protein in the pathogenesis sepsis. However, its impacts on sepsis not yet been reported. established a septic mouse model using cecal ligation puncture (CLP) gave mice either or placebo (saline). The effects serum enzyme, Evans blue leakage, lung wet-to-dry weight...

Recent studies have identified the critical roles of nucleolin in a variety cellular processes, including regulation viral replication and tumour formation. However, possible myocardial preconditioning remain undefined. We used an vivo rat ischaemic (IP) model (four cycles 5 min ischaemia 10 reperfusion) hydrogen peroxide (H2O2-PC) models. found that mRNA protein expression showed time-dependent increase during recovery rats H2O2-PC neonatal cardiomyocytes. Nucleolin overexpression enhanced...

Abstract Ovarian cancer (OC) is the most lethal gynaecological malignancy, characterized by high recurrence and mortality. However, mechanisms of its pathogenesis remain largely unknown, hindering investigation functional roles. This study sought to identify key hub genes that may serve as biomarkers correlated with prognosis. Here, we conduct an integrated analysis using weighted gene co‐expression network (WGCNA) explore clinically significant sets candidate associated OC clinical...

Abstract Background MicroRNAs (miRNAs) are widely distributed in cells and participate the regulation of pathophysiological process many diseases. As an important part non‐coding RNA, miRNAs regulate a variety molecules signal pathways tumour cells. However, evidence for regulatory mechanisms specific is still lacking. Methods In this study, we used transcriptomics analysis integrated public databases to screen that have key effects on breast cancer (BC). addition, vitro vivo studies...