A5103194244- Sugarcane Cultivation and Processing
- Plant Pathogenic Bacteria Studies
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Plant Disease Resistance and Genetics
- Biofuel production and bioconversion
- Cardiac Fibrosis and Remodeling
- Plant Virus Research Studies
- MicroRNA in disease regulation
- Cardiac electrophysiology and arrhythmias
- Traditional Chinese Medicine Analysis
- Circular RNAs in diseases
- Cardiac Ischemia and Reperfusion
- Plant Pathogens and Fungal Diseases
- Plant and Fungal Interactions Research
- Bioactive natural compounds
- Insect-Plant Interactions and Control
- Retinoids in leukemia and cellular processes
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- Cardiovascular Function and Risk Factors
- Signaling Pathways in Disease
- Macrophage Migration Inhibitory Factor
- Cardiomyopathy and Myosin Studies
- Icing and De-icing Technologies
Harbin Medical University
2010-2025
Yunnan Academy of Agricultural Sciences
2015-2024
Shanghai University
2023
Zhejiang Ocean University
2017-2018
Heilongjiang Academy of Sciences
2018
Ministry of Education of the People's Republic of China
2012-2013
Sichuan Agricultural University
2012
Université de Montréal
2010
Montreal Heart Institute
2010
Nippon Medical School
2007
A characteristic of both clinical and experimental atrial fibrillation (AF) is electric remodeling associated with profound reduction L-type Ca(2+) current shortening the action potential duration. The possibility that microRNAs (miRNAs) may be involved in this process has not been tested. Accordingly, we assessed role miRNAs regulating AF.The miRNA transcriptome was analyzed by microarray verified real-time reverse-transcription polymerase chain reaction left samples from dogs AF...
The present study was designed to decipher molecular mechanisms underlying nicotine's promoting atrial fibrillation (AF) by inducing structural remodelling. canine model of AF successfully established nicotine administration and rapid pacing. fibroblasts isolated from healthy dogs were treated with nicotine. role microRNAs (miRNAs) on the expression regulation transforming growth factor-β1 (TGF-β1), TGF-β receptor type II (TGF-βRII), collagen production evaluated in vivo vitro....
Cardiac interstitial fibrosis is a major cause of the deteriorated performance heart in patients with chronic myocardial infarction. MicroRNAs (miRs) have recently been proven to be novel class regulators cardiovascular diseases, including those associated cardiac fibrosis. This study aimed explore role miR-101 and underlying mechanisms.Four weeks after coronary artery ligation rats, expression miR-101a miR-101b (miR-101a/b) peri-infarct area was decreased. Treatment cultured rat neonatal...
The present study was designed to investigate whether the beneficial effects of β-blocker propranolol are related regulation microRNA miR-1. We demonstrated that reduced incidence arrhythmias in a rat model myocardial infarction by coronary artery occlusion. Overexpression miR-1 observed ischaemic myocardium and strikingly, administration reversed up-regulation nearly back control level. In agreement with its miR-1-reducing effect, relieved injuries during ischaemia, restored membrane...
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and high-lethality fibrotic lung disease characterized by excessive fibroblast proliferation, extracellular matrix accumulation, and, ultimately, loss of function. Although dysregulation some microRNAs (miRs) has been shown to play important roles in the pathophysiological processes IPF, role miRs diseases not well understood. In this study, we found downregulation miR-26a lungs mice with experimental which resulted...
Background: Matrine is one of the major alkaloids extracted from Sophora flavescens and has been used clinically for breast cancer with notable therapeutic efficacy in China. However, mechanisms are still largely unknown. Methods: Cell viability was analyzed by MTT assay. After MCF-7 cells were treated matrine 48h, apoptosis detected flow cytometry, TUNEL assay transmission electron microscopy, cell cycle distribution also cytometry. Further, expression PTEN, pAkt, Akt, pBad, Bad,...
Tanshinone IIA is an active component of a traditional Chinese medicine based on Salvia miltiorrhiza, which reduces sudden cardiac death by suppressing ischaemic arrhythmias. However, the mechanisms underlying anti-arrhythmic effects remain unclear.A model myocardial infarction (MI) in rats ligating left anterior descending coronary artery was used. or quinidine given daily, before (7 days) and after (3 months) MI; electrical activity monitored ECG recording. Whole-cell patch-clamp...
Tanshinone IIA is a fat-soluble pharmacologically active ingredient of Danshen, well-known traditional Chinese medicine used for cardiovascular diseases such as coronary heart disease. has been confirmed to suppress miR-1 and reduce the arrhythmogenesis after myocardial infarction (MI). However, modulation mechanism not clear. was administrated daily 7 days before ligation left anterior descending artery (LAD) lasted 3 months LAD. Neonatal cardiomyocytes were exposed 2% O2+95% N2 condition...
Abstract: Arsenic trioxide (As 2 O 3 ) achieved dramatic remissions in patients with acute promyelocytic leukaemia. Clinical reports have shown that treatment was associated cardiotoxicity. We investigated the toxic mechanisms of As H9c2 cardiomyocytes. Clinically relevant concentrations (2–10 µM) reduced viability cells a concentration‐dependent manner. The decreased cell because induced apoptosis (cell shrinkage, nuclear alterations and caspase‐3 activation), or even necrosis at higher...
Abstract A lot of evidence indicates that cardiac fibroblasts are essential for maintaining the structure and function heart. The present study examined whether TGFBR3 (transforming growth factor type III receptor, also known as betaglycan) could prevent hypoxia‐induced injury in neonatal mice fibroblasts, if so, its possible molecular targets. MTT, electron microscopy TUNEL assay were used to identify cell viability apoptosis fibroblasts. Results showed hypoxia 24 h markedly reduce by 49.8...
Pathological cardiac hypertrophy induced by adrenergic overactivation can subsequently develop to heart failure which remains as a leading cause of mortality worldwide.Tanshinone IIA is lipid-soluble pharmacologically active compound extracted from the rhizome Chinese herb Salvia miltiorrhiza, well-known traditional medicine used for treatment cardiovascular disorders.However, little know about effect Tanshinone on hypertrophy.The present study was aimed investigate whether prevents...
Abstract Although increases in cardiovascular load (pressure overload) are known to elicit ventricular remodeling including cardiomyocyte hypertrophy and interstitial fibrosis, the molecular mechanisms of pressure overload or AngII -induced cardiac fibrosis remain elusive. In this study, serpinE2/protease nexin-1 was over-expressed a model induced by pressure-overloaded via transverse aortic constriction (TAC) mouse. Knockdown serpinE2 attenuates mouse TAC. At meantime, results showed that...
Cellular senescence is a stable cell-cycle arrest induced by telomere shortening and various types of cellular stress including oxidative stress, oncogene activation, DNA damage etc. Heme oxygenase-1 (HO-1) an inducible stress-response protein that plays antioxidant anti-apoptotic effects. However, the role underlying mechanisms HO-1 in heart are largely unknown.Echocardiography was employed to detect effect on function adult mice with myocardial infarction (MI) aged mice. The markers, p53,...
Osteosarcoma is the second highest cause of cancer-related death in children and adolescents. Majority osteosarcoma patients (90%) show metastasis. Previous reports revealed that osthole showed antitumor activities via induction apoptosis inhibition proliferation. However, potential effects detailed molecular mechanisms involved remained unclear.Cell viability was analyzed by MTT assay cell lines MG-63 SAOS-2. Cell cycle detected flow cytometry. The migration invasion were evaluated wound...
The present study was designed to investigate whether microRNAs (miRNAs) are involved in atrioventricular block (AVB) the setting of myocardial ischemia (MI). A cardiac-specific miR-1 transgenic (Tg) mouse model successfully established for first time this using microinjection. level measured by real-time qRT-PCR. Whole-cell patch clamp employed record L-type calcium current (I Ca,L) and inward rectifier K(+) K1). Expression connexin 43 (Cx43) protein determined western blot analysis....
This study aims to the possibility of enhancing mechanical properties and biodegradability polylactic acid (PLA) using cyclodextrins (CD). Through melt-blending solvent casting methods, PLA/CD composites with varying content CD (0%, 1%, 3%, 5%) were produced. Mechanical biodegradation are assessed by precise instruments compared traditional PLA materials without CD. The research reveals that concentration not only enhances samples but also maintains their at a constant rate. 3% composition...
Matrine, a monomer of traditional Chinese medicine Sophora flavescens, is potential drug for treatment arrhythmia. The aim the study to elucidate protective effects matrine on arrhythmic rat induced by myocardial infarction (MI) and further explore underlying targets. Experiments were performed investigate long-term oral administration coronary ligation arrhythmia, measured in whole animals, via surface electrocardiogram (ECG). Whole-cell patch-clamp technique was used record action...
Tanshinone IIA is a lipid-soluble pharmacologically active compound extracted from the rhizome of Chinese herb Salvia miltiorrhiza, well-known traditional medicine used for treatment cardiovascular disorders. Previous studies have identified that tanshinone inhibited overexpression miR-1 in hypoxic neonatal cardiomyocytes. This study was designed to examine effects on miR-133 expression under condition. Neonatal rat cardiomyocytes were cultured environment (2% O2+93% N2+5% CO2) at 37°C 24...
Background: Arsenic trioxide (As2O3), an ancient drug used in traditional Chinese medicine, has substantial anticancer activities, especially the treatment of patients suffering from acute promyelocytic leukemia (APL); however underlying mechanisms are not well understood. Methods: MTT assay was to detect cell viability. Flow Cytometry analysis and caspase-3 activity were measure apoptosis APL cells. Caspase-3 Bax levels analyzed by western blot let-7d miR-766 determined real-time RT-PCR....