A5100966931- MicroRNA in disease regulation
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- RNA Research and Splicing
- Nerve injury and regeneration
- RNA Interference and Gene Delivery
- Fuel Cells and Related Materials
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA regulation and disease
- Reconstructive Surgery and Microvascular Techniques
- Electrospun Nanofibers in Biomedical Applications
- Cardiomyopathy and Myosin Studies
- Cardiac Fibrosis and Remodeling
- Anodic Oxide Films and Nanostructures
- Ion Channels and Receptors
- Cardiovascular Disease and Adiposity
- Alzheimer's disease research and treatments
- Management of metastatic bone disease
- Pain Mechanisms and Treatments
- Autophagy in Disease and Therapy
- Atrial Fibrillation Management and Outcomes
- Ferroptosis and cancer prognosis
- Receptor Mechanisms and Signaling
- Wound Healing and Treatments
Suizhou Central Hospital
2023-2025
Tsinghua University
2025
State Key Laboratory of New Ceramics and Fine Processing
2025
Beijing Tsinghua Chang Gung Hospital
2025
Zigong First People's Hospital
2024
Wenzhou Medical University
2019-2022
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2019-2022
Affiliated Hospital of Southwest Medical University
2019-2021
Southwest Medical University
2021
Lifetech Scientific (China)
2016
The microRNAs miR-1 and miR-133 are preferentially expressed in cardiac skeletal muscles have been shown to regulate differentiation proliferation of these cells. We report here a novel aspect cellular function regulation cardiomyocyte apoptosis. produced opposing effects on apoptosis, induced by oxidative stress H9c2 rat ventricular cells, with being pro-apoptotic anti-apoptotic. level was significantly increased response stress. identified single target sites for only, the 3′-untranslated...
A characteristic of both clinical and experimental atrial fibrillation (AF) is electric remodeling associated with profound reduction L-type Ca(2+) current shortening the action potential duration. The possibility that microRNAs (miRNAs) may be involved in this process has not been tested. Accordingly, we assessed role miRNAs regulating AF.The miRNA transcriptome was analyzed by microarray verified real-time reverse-transcription polymerase chain reaction left samples from dogs AF...
Atrial fibrillation (AF) is a highly prevalent arrhythmia with pronounced morbidity and mortality. Inward-rectifier K+ current (IK1) believed to be an important regulator of reentrant-spiral dynamics major component AF-related electrical remodeling. MicroRNA-26 (miR-26) predicted target the gene encoding KIR2.1, KCNJ2. We found that miR-26 was downregulated in atrial samples from AF animals patients this downregulation accompanied by upregulation IK1/KIR2.1 protein. overexpression suppressed...
Fibroblast proliferation and differentiation are central in atrial fibrillation (AF)-promoting remodeling. Here, we investigated fibroblast regulation by Ca(2+)-permeable transient receptor potential canonical-3 (TRPC3) channels.Freshly isolated rat cardiac fibroblasts abundantly expressed TRPC3 had appreciable nonselective cation currents (I(NSC)) sensitive to a selective TPRC3 channel blocker, pyrazole-3 (3 μmol/L). Pyrazole-3 suppressed angiotensin II-induced Ca(2+) influx, proliferation,...
We have previously found that the ether-a-go-go related gene (ERG), a long QT syndrome encoding key K(+) channel (I(Kr)) in cardiac cells, is severely depressed its expression at protein level but not mRNA diabetic subjects. The mechanisms underlying disparate alterations of ERG and mRNA, however, remained unknown. report here remarkable overexpression miR-133 hearts from rabbit model diabetes, parallel serum response factor (SRF), which known to be transactivator miR-133, was also robustly...
Recent evidence has suggested microRNAs as viable therapeutic targets for a wide range of human disease. However, lack gene-specificity microRNA actions may hinder this application. Here we developed two new approaches, the gene-specific mimic and microRNA-masking antisense to explore possibility using microRNA's principle in manner. We examined value these strategies rational approaches develop heart rate-reducing agents "biological pacemakers" by manipulating expression cardiac pacemaker...
Background— Atrial tissue fibrosis is often an important component of the atrial fibrillation (AF) substrate. Small noncoding microRNAs are mediators in many cardiac remodeling paradigms. MicroRNA-21 (miR-21) has been suggested to be ventricular fibrotic by downregulating Sprouty-1, a protein that suppresses fibroblast proliferation. The present study examined potential role miR-21 AF substrate resulting from experimental heart failure after myocardial infarction (MI). Methods and Results—...
Cardiac hypertrophy is characterized by electrical remolding with increased risk of arrhythmogenesis. Enhanced abnormal automaticity ventricular cells contributes critically to hypertrophic arrhythmias. The pacemaker current I(f), carried the hyperpolarization-activated channels encoded mainly HCN2 and HCN4 genes in heart, plays an important role determining cardiac automaticity. Their expressions reportedly increase failing hearts, contributing arrhythmogenesis under these conditions. We...
Anti-miRNA antisense inhibitors (AMOs) have demonstrated their utility in miRNA research and potential therapy. Here we report a modified AMO approach which multiple units are engineered into single unit that is able to simultaneously silence multiple-target miRNAs, the or MTg-AMO. We validated technique with two separate MTg-AMOs: anti-miR-21/anti-miR-155/anti-miR-17-5p anti-miR-1/anti-miR-133. first verified ability of MTg-AMOs antagonize repressive actions target miRNAs using luciferase...
Chronic allodynia stemming from peripheral stump neuromas can persist for extended periods, significantly compromising patients' quality of life. Conventional managements nerve stumps have demonstrated limited effectiveness in ensuring their orderly termination. In this study, we present a spatially confined conduit strategy, designed to enhance the self-organization regenerating nerves after truncation. This innovative approach elegantly enables autonomous slowing axonal outgrowth response...
Accumulating evidence demonstrated that bone marrow‐derived mesenchymal stem cells (BMSCs) may transdifferentiate into cardiomyocytes and replace apoptotic myocardium so as to improve functions of damaged hearts. However, little information is known about molecular mechanisms underlying myogenic conversion BMSCs. microRNAs endogenous noncoding small molecules function inhibit protein translation post‐transcriptionally by binding complementary sequences targeted mRNAs. Here, we reported...
Excitability is a fundamental characteristic of cardiac cells, which delicately determined by ion channel activities modulated many factors. MicroRNA (miRNA) expression dynamically regulated and altered miRNA can render deregulation genes leading to channelopathies-arrhythmogenesis. Indeed, evidence has emerged indicating the crucial role miRNAs in controlling excitability regulating at post-transcriptional level. However, very limited experimental data literature hinder our understanding...
Fibroblasts are involved in cardiac arrhythmogenesis and contribute to the atrial fibrillation substrate congestive heart failure (CHF) by generating tissue fibrosis. display robust ion currents, but their functional importance is poorly understood.To characterize fibroblast inward-rectifier K(+) current (IK1) remodeling CHF its effects on properties.Freshly isolated left fibroblasts were obtained from controls dogs with (ventricular tachypacing). Patch clamp was used record resting membrane...
In cardiac cells, KCNQ1 assembles with KCNE1 and forms a channel complex constituting the slow delayed rectifier current I(Ks). Expression of are regionally heterogeneous changes pathological states heart. The aims this study were to decipher molecular mechanisms for transcriptional post-transcriptional regulation expression genes shed light on their spatial heterogeneity distribution. We cloned 5'-flanking region identified transcription start sites gene. characterized core promoters...
Inhibition of individual K(+) currents causes functionally based compensatory increases in other that minimize changes action potential duration, a phenomenon known as repolarization reserve. The possibility sustained channel inhibition may induce remodeling ion current expression has not been tested. Accordingly, we assessed the effects one on various cardiac ionic currents.Adult canine left ventricular cardiomyocytes were incubated primary culture and paced at physiological rate (1 Hz) for...
Abnormal QT prolongation (QT-P) in diabetic patients has become a nonnegligible clinical problem and attracted increasing attention from basic scientists, because it increases the risk of lethal ventricular arrhythmias. Correction QT-P may be an important measure minimizing sudden cardiac death patients. Here we report efficacy insulin preventing associated arrhythmias mechanisms underlying effects rabbit model type 1 insulin-dependent diabetes mellitus (IDDM). The heart rate-corrected (QTc)...
ABSTRACT Even though endobronchial lipoma is rare, it can mimic malignancy and lead to significant complications such as progressive dyspnea subsequent lung infections related obstruction. We present a case of located in the trachea causing almost complete tracheal The patient's disappeared after undergoing bronchoscopic excision mass.