A5022412641- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Cancer-related gene regulation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Advanced Measurement and Metrology Techniques
- Signaling Pathways in Disease
- Viral Infectious Diseases and Gene Expression in Insects
- Research on Leishmaniasis Studies
- Pluripotent Stem Cells Research
- Phagocytosis and Immune Regulation
- RNA Interference and Gene Delivery
- Traffic Prediction and Management Techniques
- Biochemical and Molecular Research
- Railway Systems and Energy Efficiency
- Hepatitis B Virus Studies
- Surface Roughness and Optical Measurements
- Travel-related health issues
Sun Yat-sen University
2018-2025
Anhui Medical University
2015-2025
First Affiliated Hospital of Anhui Medical University
2015-2025
Ministry of Education of the People's Republic of China
2025
The First Affiliated Hospital, Sun Yat-sen University
2024
Tianjin University
2022
The Seventh Affiliated Hospital of Sun Yat-sen University
2021-2022
Beijing Jiaotong University
2006
Entry Exit Inspection and Quarantine Bureau
2005
The N6-methyladenosine (m6A) modification influences various mRNA metabolic events and tumorigenesis, however, its functions in nonsense-mediated decay (NMD) whether NMD detects induced carcinogenesis pathways remain undefined. Here, we showed that the m6A methyltransferase METTL3 sustained oncogenic role by modulating of splicing factors alternative isoform switches glioblastoma (GBM). Methylated RNA immunoprecipitation-seq (MeRIP-seq) analyses peaks were enriched at pathway-related...
N6 -methyladenosine (m6 A) modification of mRNA mediates diverse cellular and viral functions. Infection with Epstein-Barr virus (EBV) is causally associated nasopharyngeal carcinoma (NPC), 10% gastric carcinoma, various B-cell lymphomas, in which the latent lytic phases both play vital roles. Here, we show that EBV transcripts exhibit differential m6 A human NPC biopsies, patient-derived xenograft tissues, cells at different infection stages. A-modified are recognized destabilized by YTHDF1...
Abstract N6-methyladenosine (m6A) is a reversible and dynamic RNA modification in eukaryotes. However, how cells establish cell-specific m6A methylomes still poorly understood. Here, we developed computational framework to systematically identify trans regulators of through integrating gene expressions, binding targets motifs large number proteins (RBPs) with co-methylation network constructed using large-scale across diverse cell states. We applied the successfully identified 32...
Toxoplasma gondii is an intracellular protozoan that affects most species of endothermic animals including humans with a great infection rate. The vertical transmission T. causes abortion, constituting serious threat to and leading losses in livestock production. Distinct from population structure North America Europe, Chinese 1 (ToxoDB #9) dominant genotype prevalent China. Among the isolates 1, Wh3 Wh6 have different virulence pathogenicity mice. However, little has been known about their...
Lesch-Nyhan syndrome (LNS, OMIM #300322) is a rare X-linked genetic disorder caused by variants in the HPRT1 gene, which codes for Hypoxanthine-guanine phosphoribosyltransferase (HGPRT). gene disrupt normal purine metabolism, leading to involvement of multiple organ systems, primarily characterized hyperuricemia, dystonia, and neurological abnormalities, makes LNS clinically heterogeneous diagnostically challenging. Here, we report case 27-year-old Chinese male exhibiting severe lower limb...
N6-methyladenosine (m6A) stands as the predominant modification in eukaryotic mRNA and is involved various biological functions. Aberrant m6A has been implicated abnormal cellular phenotypes, including defects stem cell differentiation tumorigenesis. However, precise effects of on proliferation underlining mechanism metabolic gene regulation remain incompletely understood. Here, we established a environment with low-m6A levels observed severe impairment proliferation. Mechanistic studies...
Abstract Deficiency of the N 6 ‐methyladenosine (m A) methyltransferase complex results in global reduction m A abundance and defective cell development embryonic stem cells (ESCs). However, it's unclear whether regional methylation affects fate decisions due to inability modulate individual modification ESCs with precise temporal control. Here, a targeted RNA erasure (TRME) system is developed achieve site‐specific demethylation RNAs human (hESCs). TRME, which stably transfected,...
Abstract N 6 -methyladenosine (m A) plays important role in lineage specifications of embryonic stem cells. However, it is still difficult to systematically dissect the specific m A sites that are essential for early differentiation. Here, we develop an adenine base editor-based strategy identify functional control decisions human We design 7999 sgRNAs targeting 6048 screen act as either boosters or barriers definitive endoderm specification 78 enriched non-definitive cells and 137...
Toxoplasma gondii infection evokes a strong Th1-type response with IL-12 and IFN-γ secretion. Recent studies suggest that the of pregnant mice T. may lead to adverse pregnancy results caused by subversion physiological immune tolerance at maternofetal interface rather than direct invasion parasite. Genotype-associated dense granule protein GRA15 II tends induce classically activated macrophage (M1) differentiation subsequently activating NK, Th1 Th17 cells whereas rhoptry ROP16I/III drives...
Abstract Background Canonical nonsense-mediated decay (NMD) is an important splicing-dependent process for mRNA surveillance in mammals. However, processed pseudogenes are not able to trigger NMD due their lack of introns. It largely unknown whether they have evolved other mechanisms. Results Here, we find that the RNAs pseudogenes, especially dramatically higher m 6 A levels than cognate protein-coding genes, associated with de novo peaks and motifs human cells. Furthermore, rapidly...
Toxoplasma gondii is an opportunistic protozoan apicomplexan and obligate intracellular parasite that infects a wide range of animals humans. Rhoptry proteins 5 (ROP5), ROP16, ROP18 dense granules 15 (GRA15) are the important effectors secreted by T. which link to strain virulence for mice modulate host's response parasite. Little has been known about these molecules as well GRA3 in type Chinese 1 strains show polymorphism among archetypical genotypes. This study examined genetic diversity...
Abstract Cardiac development involves large‐scale rearrangements of the proteome. How developing cardiac cells maintain integrity proteome during rapid lineage transition remains unclear. Here it is shown that proteotoxic stress visualized by misfolded and/or aggregated proteins appears early differentiation human pluripotent stem and resolved activation PERK branch unfolded protein response (UPR). depletion increases accumulation, leading to pluripotency exit defect impaired mesendoderm...
Quantitative polymerase chain reaction (qPCR) analysis is a precise and effective method for the study of mRNA expression throughout field peripheral blood mononuclear cell (PBMC) research. However, use suitable reference genes data normalization critical to obtain meaningful reproducible results. The present aimed identify greatest further research in PBMC Chronic Hepatitis B (CHB) patients.We assessed stability four commonly used (beta actin, beta-tubulin, 18S rRNA, GAPDH) CHB patients....
ABSTRACT Various genome-wide and transcriptome-wide technologies are based on antibodies, however, the specificity of antibodies different targets has not been characterized or considered in analyses. The antibody-based MeRIP-seq is most widely used method to determine locations N6-methyladenosine (m 6 A) RNAs, especially for differential m A However, antibody specificities RNA regions their resulting technical biases analyses have evaluated. Here, we evaluated using 100 pairs spike-in RNAs...
The primary aim of this study is to measure the JAK-STAT signaling in HBV infected peripheral blood mononuclear cells (PBMCs) stimulated by IFN-α and 3-TC explore influence JAKSTAT pathways.PBMCs were separated from healthy volunteers patients who had not received any treatment with chronic hepatitis B. PBMCs divided into control group, stimulation Lamivudine combined group. expression molecules signal transduction pathway (STAT1, STAT2, IRF9) antiviral protein (MxA) detected RT-qPCR Western...
A major clinical challenge for treating patients with pancreatic ductal adenocarcinoma (PDAC) is identifying those that may benefit from adjuvant chemotherapy versus will not. Thus, there a need robust and convenient biomarker predicting response in PDAC patients. In this study, network inference was conducted by integrating the differentially expressed cell cycle signatures target genes between basal-like subtype classical of PDAC. As result statistical analysis, two dominant genes, RASAL2...
<p>Supplementary Figures and Methods</p>
<p>Supplementary Figures and Methods</p>