A5040988785- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Computational Drug Discovery Methods
- Saffron Plant Research Studies
- Synthesis and biological activity
- Peptidase Inhibition and Analysis
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- HVDC Systems and Fault Protection
- Medicinal Plants and Bioactive Compounds
- Synthesis and Biological Evaluation
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Parasitic Infections and Diagnostics
- GABA and Rice Research
- Cytokine Signaling Pathways and Interactions
- Biological and pharmacological studies of plants
- Genetic Syndromes and Imprinting
- 3D Printing in Biomedical Research
- Cancer-related gene regulation
- Cholinesterase and Neurodegenerative Diseases
- Antifungal resistance and susceptibility
Shenyang Pharmaceutical University
2014-2024
Guangzhou Medical University
2022-2023
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2020
Sun Yat-sen University
2019
Zhejiang University
2013
Abstract Oncolytic adenovirus (Ad) infection promotes intracellular autophagy in tumors. This could kill cancer cells and contribute to Ads-mediated anticancer immunity. However, the low intratumoral content of intravenously delivered Ads be insufficient efficiently activate tumor over-autophagy. Herein, we report bacterial outer membrane vesicles (OMVs)-encapsulating as microbial nanocomposites that are engineered for autophagy-cascade-augmented immunotherapy. Biomineral shells cover...
Abstract Parthanatos is one of the major pathways programmed cell death in ischemic stroke characterized by DNA damage, poly (ADP-ribose) polymerases (PARP) activation, and (PAR) formation. Here we demonstrate that crocetin, a natural potent antioxidant compound from Crocus sativus , antagonizes parthanatos stroke. We reveal mechanistically, crocetin inhibits NADPH oxidase 2 (NOX2) activation to reduce reactive oxygen species (ROS) PAR production at early stage parthanatos. Meanwhile...
Abstract N6-methyladenosine (m6A) is a reversible and dynamic RNA modification in eukaryotes. However, how cells establish cell-specific m6A methylomes still poorly understood. Here, we developed computational framework to systematically identify trans regulators of through integrating gene expressions, binding targets motifs large number proteins (RBPs) with co-methylation network constructed using large-scale across diverse cell states. We applied the successfully identified 32...
Herein, we report the discovery and characterization of a novel class PAK4 inhibitors with quinazoline scaffold. Based on shape chemical composition ATP-binding pocket PAKs, chose 2,4-diaminoquinazoline series as starting point. Guided by X-ray crystallography structure-based drug design (SBDD) approach, 4-aminoquinazoline-2-carboxamide were designed synthesized. The inhibitors' selectivity, therapeutic potency, pharmaceutical properties optimized. One best compounds, 31 (CZh226), showed...
Because the tumor-draining lymph nodes (TDLNs) microenvironment is commonly immunosuppressive, oncolytic microbe-induced tumor antigens aren't sufficiently cross-primed specific T cells through antigen-presenting (e.g., dendritic (DCs)) in TDLNs. Herein, this work develops micro-to-nano microbial therapeutics based on pyranose oxidase (P
Gastrodin (GAS) is the main chemical component of traditional Chinese herb Gastrodia elata (called “Tianma” in Chinese), which has been used to treat neurological conditions, including headaches, epilepsy, stroke, and memory loss. To our knowledge, it unclear whether GAS a therapeutic effect on Huntington’s disease (HD). In present study, we evaluated degradation mutant huntingtin protein (mHtt) by using PC12 cells transfected with N-terminal mHtt Q74. We found that 0.1–100 μM had no...
Tandem repeats (TRs) are abundant and widely distributed in eukaryotic genomes. TRs thought to have various functions gene transcription, DNA methylation, nucleosome position chromatin organization. Variation of repeat units the genome is observed association with a number diseases, such as Fragile X Syndrome, Huntington's disease Friedreich's ataxia. However, underlying mechanisms involved poorly understood, largely owing technical limitations modification at definite sites vivo....
Abstract p21‐Activated kinase 4 (PAK4) is a serine/threonine protein that plays important roles in wide variety of human diseases including cancer. Targeting this with specific inhibitors great interest the treatment In study, PAK4 and its interaction ATP‐competitive was investigated by combined ligand‐ structure‐based approach. First, ligand‐based pharmacophore model generated, consisting five chemical features: positive ionizable center, two hydrophobic groups, hydrogen bond donor,...
Novel PAK4 inhibitors were discovered using structure-based virtual screening approach for further chemical modification.
Discovery of a new class natural product-inspired PAK4 inhibitors as potent anti-cancer agents.
Genomic imprinting in mammals causes certain genes to be expressed according their parental origin and thereby prevents parthenogenesis. However, the evolutionary significance of genomic remains unclear. Here, we study methylation status ntl, a developmentally decisive gene, egg, sperm early embryos at various developmental stages bisexual diploid fish unisexual polyploid fish. Bisulfite sequencing analysis revealed that maternal-specific occurs ntl promoter during gametogenesis fish, where...
Consumption of natto, a traditional eastern Asian food made fermented soybeans by <em>Bacillus Subtilis</em>, has long been linked to healthy aging and longer human lifespan. As the key thrombolytic ingredient serine protease nattokinase (NK) developed into widely-used dietary supplement. NK shown excellent anti-thrombus, thrombolytic, anti-inflammation activities that potentially delay provide therapeutic effects on aging-related diseases. In this review, we critically overview experimental...
A combined ligand- and target-based approach was used to analyse the interaction models of Cryptosporidium parvum inosine 5’-monophosphate dehydrogenase (CpIMPDH) with selective inhibitors. First, a ligand-based pharmacophore model generated from 20 NAD+ competitive CpIMPDH inhibitors HipHop module. The characteristic binding site then described, modes representative were studied by molecular docking. combination docking results allowed us evaluate features structural information CpIMPDH....
Abstract Parthanatos is one of the major types programmed cell death in ischemic stroke characterized by DNA damage, poly (ADP-ribose) polymerases (PARP) activation, and (PAR) formation. Here we demonstrate that crocetin, a natural potent antioxidant compound from Crocus sativus , antagonizes parthanatos stroke. We reveal mechanistically, crocetin inhibits NADPH oxidase 2 (NOX2) activation to reduce reactive oxygen species (ROS) PAR production at early stage parthanatos. Meanwhile PARylated...