A5064040167- RNA modifications and cancer
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- HVDC Systems and Fault Protection
- Viral-associated cancers and disorders
- RNA Research and Splicing
Sun Yat-sen University
2021-2025
Abstract Serine/arginine-rich splicing factor 7 (SRSF7), a known factor, has been revealed to play oncogenic roles in multiple cancers. However, the mechanisms underlying its have not well addressed. Here, based on N 6-methyladenosine (m 6 A) co-methylation network analysis across diverse cell lines, we find that gene expression of SRSF7 is positively correlated with glioblastoma (GBM) cell-specific m6A methylation. We then indicate novel regulator, which specifically facilitates methylation...
ABSTRACT Although plenty of machine learning models have been developed to detect m 6 A RNA modification sites using the electric current signals ONT direct sequencing (DRS) reads, landscape on different isoforms is still a mystery due their limited capacity distinguish individual long reads and isoforms. The primary challenge in training model with single-read accuracy difficulty obtaining data from DRS that comprehensively represent endogenous RNAs. Here, we endogenously label methylated...
ABSTRACT Various genome-wide and transcriptome-wide technologies are based on antibodies, however, the specificity of antibodies different targets has not been characterized or considered in analyses. The antibody-based MeRIP-seq is most widely used method to determine locations N6-methyladenosine (m 6 A) RNAs, especially for differential m A However, antibody specificities RNA regions their resulting technical biases analyses have evaluated. Here, we evaluated using 100 pairs spike-in RNAs...
Abstract Serine/Arginine-Rich Splicing Factor 7 (SRSF7), which is previously recognized as a splicing factor, has been revealed to play oncogenic roles in multiple cancers. However, the mechanisms underlying its have not well addressed. Here, based on N6-methyladenosine (m 6 A) co-methylation network analysis across diverse cell lines, we found SRSF7 positively correlated with glioblastoma cell-specific m A methylation. We then proved novel regulator that specifically facilitates methylation...