A5089038231- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Bacteriophages and microbial interactions
- Cellular transport and secretion
- Vibrio bacteria research studies
- RNA and protein synthesis mechanisms
- Antimicrobial Peptides and Activities
- Erythrocyte Function and Pathophysiology
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Algal biology and biofuel production
- Biopolymer Synthesis and Applications
- RNA modifications and cancer
- Marine Toxins and Detection Methods
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Lipid metabolism and biosynthesis
- Legionella and Acanthamoeba research
- Microbial Community Ecology and Physiology
Loewe Center for Synthetic Microbiology
2016-2025
Philipps University of Marburg
2020-2025
Ruhr University Bochum
2018
The alarmones (p)ppGpp play a critical role in chloroplasts by acting as signalling molecules that regulate gene expression, protein synthesis and chloroplast ( cp ) development, particularly response to stress nutrient availability. However, the underlying molecular mechanisms are still poorly understood. Here, we show binds GTPase‐containing NG domains of signal recognition particle (SRP) its receptor, preventing their GTP‐dependent association through competitive mechanism. structure...
Abstract Acetyl-CoA synthetase (Acs) generates acetyl-coenzyme A (Ac-CoA) but its excessive activity can deplete ATP and lead to a growth arrest. To prevent this, Acs is regulated through Ac-CoA-dependent feedback inhibition executed by acetyltransferases such as AcuA in Bacillus subtilis . acetylates the catalytic lysine of AcsA turning inactive. Here, we report that form tightly intertwined complex – C-terminal domain binds acetyltransferase AcuA, while C-terminus occupies CoA-binding site...
Second messengers are free to diffuse through the cells and activate all responsive elements. Cyclic di-GMP (c-di-GMP) signaling plays an important role in determination of life style transition between motility sessility/biofilm formation but involves numerous distinct synthetases (diguanylate cyclases [DGCs]) or receptor pathways that appear act independent manner. Using Bacillus subtilis as a model organism, we show for two c-di-GMP pathways, DGCs molecules operate via direct...
Abstract Stressosomes are stress-sensing protein complexes widely conserved among bacteria. Although a role in the regulation of general stress response is well documented Gram-positive bacteria, activating signals still unclear, and little known about physiological function stressosomes Gram-negative Here we investigated stressosome marine pathogen Vibrio vulnificus . We demonstrate that it senses oxygen identified its modulating iron-metabolism. determined cryo-electron microscopy...
The structure of bacterial biofilms is predominantly established through the secretion extracellular polymeric substances (EPS). They show that Bacillus subtilis contains an operon (ydaJ-N) whose induction leads to increased Congo Red staining and strongly altered biofilm architecture, suggesting it mediates production unknown exopolysaccharide. Supporting this idea, overproduction YdaJKLMN cell clumping during exponential growth in liquid culture, also causes colony morphology alterations...
The universal and pleiotropic cyclic dinucleotide second messenger c-di-GMP is most prominently known to inversely regulate planktonic sessile lifestyles of Gram-negative species. In the Gram-positive model organism Bacillus subtilis, intracellular levels are modulated by a concise set three diguanylate cylases (DgcK, DgcP, DgcW) one phosphodiesterase (PdeH). Two recent studies have reported negative influence receptor DgrA (PilZ domain protein) on swarming motility indicating conserved role...
Abstract Flagellin is amongst the most abundant proteins in flagellated bacterial species and constitutes major building block of flagellar filament. The FliW FliS serve post-transcriptional control flagellin guide protein to type III secretion system (fT3SS), respectively. Here, we present high-resolution structure FliS/flagellin heterodimer show that bind opposing interfaces located at N- C-termini flagellin. FliS/flagellin/FliW heterotrimer able interact with FlhA-C suggesting are...
Abstract In the chloroplast, 54 kDa subunit of signal recognition particle (cpSRP54) is involved in post-translational transport light-harvesting chlorophyll a/b-binding proteins (LHCPs) and co-translational plastid-encoded subunits photosynthetic complexes to thylakoid membrane. It forms a high-affinity complex with plastid-specific cpSRP43 for transport, while ribosome-associated pool coordinates its function. CpSRP54 constitutes conserved multidomain protein, comprising GTPase (NG)...
The ubiquitous bacterial second messenger bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is involved in the regulation of numerous processes including biofilm formation, motility, virulence, cell cycle and differentiation. In this study, we searched genome ecologically important marine alphaproteobacterium Dinoroseobacter shibae DFL12T for genes encoding putative c-di-GMP-modulating enzymes. Overall, D. was found to possess two diguanylate cyclases (Dshi_2814 Dshi_2820) as well c-di-GMP-specific...
Abstract Bacillus subtilis can form structurally complex biofilms on solid or liquid surfaces, which requires expression of genes for matrix production. The transcription these is activated by regulatory protein RemA, binds to poorly conserved, repetitive DNA regions but lacks obvious DNA-binding motifs domains. Here, we present the structure RemA homologue from Geobacillus thermodenitrificans , showing a unique octameric ring with potential 16-meric superstructure. These results, together...
Abstract In the chloroplast, 54 kDa subunit of signal recognition particle (cpSRP54) is involved in posttranslational transport light-harvesting chlorophyll a/b-binding proteins (LHCPs) and cotranslational plastid-encoded subunits photosynthetic complexes to thylakoid membrane. It forms a high-affinity complex with plastid-specific cpSRP43 for transport, while ribosome-associated pool coordinates its function. CpSRP54 constitutes conserved multidomain protein, comprising GTPase (NG)...
Biofilms can be viewed as tissue-like structures in which microorganisms are organized a spatial and functional sophisticated manner. Biofilm formation requires the orchestration of highly integrated network regulatory proteins to establish cell differentiation production complex extracellular matrix. Here, we discuss role essential Bacillus subtilis biofilm activator RemA. Despite intense research on biofilms, RemA is largely underappreciated protein. forms donut-shaped octamers with...