A5002055829- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- Nanocluster Synthesis and Applications
- RNA modifications and cancer
- Plant Stress Responses and Tolerance
- Advanced Nanomaterials in Catalysis
- Algal biology and biofuel production
- Adenosine and Purinergic Signaling
- Lipid metabolism and biosynthesis
- Enzyme Structure and Function
Loewe Center for Synthetic Microbiology
2020-2025
Philipps University of Marburg
2022-2025
Peruvian University of Applied Sciences
2019-2020
When bacteria experience growth-limiting environmental conditions, the synthesis of hyperphosphorylated guanosine derivatives (p)ppGpp is induced by enzymes RelA/SpoT homology (RSH)-type protein family. High levels induce a process called 'stringent response', major cellular reprogramming during which rRNA and tRNA downregulated, stress-related genes upregulated, mRNA stability translation altered, allocation scarce resources optimized. The (p)ppGpp-mediated stringent response thus often...
The alarmones (p)ppGpp play a critical role in chloroplasts by acting as signalling molecules that regulate gene expression, protein synthesis and chloroplast ( cp ) development, particularly response to stress nutrient availability. However, the underlying molecular mechanisms are still poorly understood. Here, we show binds GTPase‐containing NG domains of signal recognition particle (SRP) its receptor, preventing their GTP‐dependent association through competitive mechanism. structure...
During host colonization, bacteria use the alarmones (p)ppGpp to reshape their proteome by acting pleiotropically on DNA, RNA, and protein synthesis. Here, we elucidate how initiating ribosome senses cellular pool of guanosine nucleotides regulates progression towards Our results show that affinity triphosphate (GTP) inhibitory concentration ppGpp for 30S-bound initiation factor IF2 vary depending programmed mRNA. The TufA mRNA enhanced GTP 30S complexes, resulting in improved tolerance...
Abstract In the chloroplast, 54 kDa subunit of signal recognition particle (cpSRP54) is involved in post-translational transport light-harvesting chlorophyll a/b-binding proteins (LHCPs) and co-translational plastid-encoded subunits photosynthetic complexes to thylakoid membrane. It forms a high-affinity complex with plastid-specific cpSRP43 for transport, while ribosome-associated pool coordinates its function. CpSRP54 constitutes conserved multidomain protein, comprising GTPase (NG)...
Eukaryotic cells transcribe ribosomal RNA and largely assemble ribosomes in a structure called the nucleolus, where chromosomal regions containing rRNA operons are clustered. In bacteria, many cluster close to origin that positioned on outer borders of nucleoids, polar areas, translating 70S located. Because nucleoids contain highest accumulation polymerase, it has been hypothesized bacteria "nucleolus-like" structures. However, ribosome subunits freely diffuse through entire cells, could...
Abstract In the chloroplast, 54 kDa subunit of signal recognition particle (cpSRP54) is involved in posttranslational transport light-harvesting chlorophyll a/b-binding proteins (LHCPs) and cotranslational plastid-encoded subunits photosynthetic complexes to thylakoid membrane. It forms a high-affinity complex with plastid-specific cpSRP43 for transport, while ribosome-associated pool coordinates its function. CpSRP54 constitutes conserved multidomain protein, comprising GTPase (NG)...
Abstract During host colonization, bacteria use the alarmone (p)ppGpp to reshape its proteome by acting pleiotropically on RNA and protein synthesis. Here, we elucidate how translation Initiation Factor 2 (IF2) senses cellular ppGpp GTP ratio regulates progression towards Our results show that affinity of inhibitory concentration for 30S-bound IF2 vary depending programmed mRNA. Highly translated mRNAs enhanced 30S complexes, resulting in fast transitions elongation Less demanded allowed...