A5005522055- Enzyme Production and Characterization
- Seaweed-derived Bioactive Compounds
- Microbial Metabolites in Food Biotechnology
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Protein Hydrolysis and Bioactive Peptides
- Vanadium and Halogenation Chemistry
- Marine and coastal plant biology
- Polyamine Metabolism and Applications
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Marine Biology and Environmental Chemistry
- Phytochemical Studies and Bioactivities
- Studies on Chitinases and Chitosanases
- Natural product bioactivities and synthesis
- Marine Sponges and Natural Products
- Natural Products and Biological Research
- Biopolymer Synthesis and Applications
- TGF-β signaling in diseases
- Oxidative Organic Chemistry Reactions
- Advanced Proteomics Techniques and Applications
- Probiotics and Fermented Foods
- Molecular Biology Techniques and Applications
- Diatoms and Algae Research
- Crystallization and Solubility Studies
Station Biologique de Roscoff
2010-2025
Sorbonne Université
2010-2025
Centre National de la Recherche Scientifique
2010-2025
Laboratoire de Biologie Intégrative des Modèles Marins
2017-2024
Laboratoire des Biomolécules
2010
Abstract Macroalgae contribute substantially to primary production in coastal ecosystems. Their biomass, mainly consisting of polysaccharides, is cycled into the environment by marine heterotrophic bacteria using largely uncharacterized mechanisms. Here we describe complete catabolic pathway for carrageenans, major cell wall polysaccharides red macroalgae, bacterium Zobellia galactanivorans . Carrageenan catabolism relies on a multifaceted carrageenan-induced regulon, including non-canonical...
Summary The genomic data on heterotrophic marine bacteria suggest the crucial role that microbes play in global carbon cycle. However, massive presence of hypothetical proteins hampers our understanding mechanisms by which this cycle is carried out. Moreover, from microorganisms are essentially annotated light biochemical knowledge accumulated and fungi decompose terrestrial plants. However algal polysaccharides clearly differ their counterparts, associated enzymes usually constitute novel...
Carrageenans are sulfated α-1,3-β-1,4-galactans found in the cell wall of some red algae that practically valuable for their gelation and biomimetic properties but also serve as a potential carbon source marine bacteria. Carbohydrate degradation has been studied extensively terrestrial plant/bacterial systems, sulfation is not present these cases, meaning enzymes used to degrade carrageenans must possess unique features recognize modifications. To gain insights into features, we have focused...
Carrageenans are sulfated polysaccharides found in the cell wall of certain red seaweeds. They widely used food industry for their gelling and stabilizing properties. In nature, carrageenans undergo enzymatic modification degradation by marine organisms. Characterizing these enzymes is crucial understanding carrageenan utilization may eventually enable development targeted processes to modify industrial applications. our study, we characterized a κ-carrageenan sulfatase, AMOR_S1_16A,...
Abstract Background The production of stable and soluble proteins is one the most important steps prior to structural functional studies biological importance. We investigated parallel in a medium throughput strategy genes coding for from various marine organisms, using protocols that involved recombinatorial cloning, protein expression screening batch purification. This was applied order respond need post-genomic validation recent success large number genomic projects. Indeed, upcoming...
Marine polysaccharide degrading enzymes, and iota-carrageenases in particular, have received little attention the past, although their substrate specificity is of interest for biotechnological applications. This mostly a consequence lack data about occurrence marine environment. Recent metagenomic mining genome sequencing bacterium, Zobellia galactanivorans, led to identification three new iota-carrageenase genes belonging glycoside hydrolase family GH82. The additional sequences helped...
Abstract Alginate is a major compound of brown macroalgae and as such an important carbon energy source for heterotrophic marine bacteria. Despite the rather simple composition alginate only comprising mannuronate guluronate units, these bacteria feature complex alginolytic systems that can contain up to seven lyases. This reflects necessity large enzyme complete degradation abundant substrate. Numerous lyases have been characterized. They belong different polysaccharide lyase (PL) families,...
Abstract Laminarin, a β(1,3)‐glucan, serves as storage polysaccharide in marine microalgae such diatoms. Its abundance, water solubility and simple structure make it an appealing substrate for bacteria. Consequently, many bacteria have evolved strategies to scavenge decompose laminarin, employing carbohydrate‐binding modules (CBMs) crucial components. In this study, we characterized two previously unassigned domains laminarin‐binding CBMs multimodular proteins from the bacterium...
The Tetraconata (Pancrustacea) concept proposes that insects are more closely related to aquatic crustaceans than terrestrial centipedes or millipedes. question therefore arises whether have kept crustacean-specific genetic traits could be targeted by specific toxins. Here we show a toxin (nigritoxin), originally identified in bacterial pathogen of shrimp, is lethal for organisms within the and non-toxic other animals. X-ray crystallography reveals nigritoxin possesses new protein fold α/β...
X-ray diffraction of native bromoperoxidase II (EC 1.11.1.18) from the brown alga Ascophyllum nodosum reveals at a resolution 2.26 Å details orthovanadate binding and homohexameric protein organization. Three dimers interwoven in contact regions tightened by hydrogen-bond-clamped guanidinium stacks along with regularly aligned water molecules form basic structure enyzme. Intra- intermolecular disulfide bridges further stabilize enzyme preventing altogether denaturing up to temperature 90 °C,...
SusC/D-like proteins are essential components of glycan utilization machineries in Bacteroidota , but remain unknown other bacterial phyla. The glycan-binding SusD-like protein forms a lid on top the SusC-like TonB-dependent transporter (TBDT) and both structurally designed to function as complex sugar uptake. In comparison, Gammaproteobacteria import glycans using classical TBDTs without an accessory protein. We have now identified TBDT fructan polysaccharide locus (PUL) marine...
Abstract The ß-(1,3)-glucan laminarin functions as storage polysaccharide in marine stramenophiles such diatoms. Laminarin is abundant, water-soluble and structured simply, making it an attractive substrate for bacteria. As a consequence, many bacteria have developed competitive strategies to scavenge decompose laminarin, which involves carbohydrate-binding modules (CBMs) key players. We therefore functionally structurally characterized two yet unassigned domains laminarin-binding CBMs...