A5052381132- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Viral Infectious Diseases and Gene Expression in Insects
- Marine and coastal ecosystems
- Marine Bivalve and Aquaculture Studies
- Bacterial Genetics and Biotechnology
Universitätsmedizin Greifswald
2021-2024
Helmholtz Centre for Infection Research
2024
Universität Greifswald
2018-2022
Institute of Marine Biotechnology
2018
Abstract Laminarin, a β(1,3)‐glucan, serves as storage polysaccharide in marine microalgae such diatoms. Its abundance, water solubility and simple structure make it an appealing substrate for bacteria. Consequently, many bacteria have evolved strategies to scavenge decompose laminarin, employing carbohydrate‐binding modules (CBMs) crucial components. In this study, we characterized two previously unassigned domains laminarin‐binding CBMs multimodular proteins from the bacterium...
Outer membrane extensions are common in many marine bacteria. However, the function of these surface enlargements or extracellular compartments is poorly understood. Using a combined approach microscopy and subproteome analyses, we therefore examined Pseudoalteromonas distincta ANT/505, an Antarctic polysaccharide degrading gamma-proteobacterium. P. produced outer vesicles (MV) vesicle chains (VC) on non-polysaccharide carbon sources during exponential stationary growth phase. Surface...
Target proteins in biotechnological applications are highly diverse. Therefore, versatile flexible expression systems for their functional overproduction required. In order to find the right heterologous gene strategy, suitable host-vector systems, which combine different genetic circuits, useful. this study, we designed a novel Bacillus subtilis toolbox, allows and secretion of potentially toxic enzymes. This toolbox comprises set 60 vectors, two promoter variants, four strong signals,...
Marine algae are responsible for half of the global primary production, converting carbon dioxide into organic compounds like carbohydrates. Particularly in eutrophic waters, they can grow massive algal blooms. This polysaccharide rich biomass represents a cheap and abundant renewable source. In nature, diverse group polysaccharides is decomposed by highly specialized microbial catabolic systems. We elucidated complete degradation pathway green algae-specific ulvan previous studies using...