A5101971834- Enzyme Structure and Function
- Advanced X-ray Imaging Techniques
- Protein Structure and Dynamics
- X-ray Spectroscopy and Fluorescence Analysis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Mass Spectrometry Techniques and Applications
- Particle Accelerators and Free-Electron Lasers
- Bacteriophages and microbial interactions
- Medical Imaging Techniques and Applications
- Bacterial Genetics and Biotechnology
- Polysaccharides and Plant Cell Walls
- Welding Techniques and Residual Stresses
- RNA Research and Splicing
- Vibrio bacteria research studies
- Metallurgy and Material Forming
- Porphyrin Metabolism and Disorders
- Microbial Metabolites in Food Biotechnology
- Photosynthetic Processes and Mechanisms
- Escherichia coli research studies
- Microstructure and Mechanical Properties of Steels
Synchrotron soleil
2010-2025
Colgate University
2020
University of Wisconsin–Milwaukee
2020
Territoires
2019
European Molecular Biology Laboratory
1993-2006
European Synchrotron Radiation Facility
1996
Daresbury Laboratory
1982-1995
Wesleyan University
1993
Birkbeck, University of London
1986
Keele University
1982
Magnetostrictive composite known as magnetostrictive nanocellulose membrances (MNMs) were fabricated by embedding Terfeonol-D particles into cellulose nanofibers (CNF). MNMs inherit flexibility and biodegradability from CNF while exhibiting magnetomechanical responses; such, the valuable rare-earth (Terfenol-D) can be recycled. Various orientations of Terfenol-D induced in MNMs, those with in-plane alignment showed strongest effect but lowest Villari effect. Materials such a unique...
Summary The genomic data on heterotrophic marine bacteria suggest the crucial role that microbes play in global carbon cycle. However, massive presence of hypothetical proteins hampers our understanding mechanisms by which this cycle is carried out. Moreover, from microorganisms are essentially annotated light biochemical knowledge accumulated and fungi decompose terrestrial plants. However algal polysaccharides clearly differ their counterparts, associated enzymes usually constitute novel...
Abstract The replication of the two chromosomes in pathogenic bacterium Vibrio cholerae is coordinated by binding initiator protein RctB to a checkpoint sequence, crtS . Replication on primary chromosome (Chr1) triggers secondary (Chr2), but details are poorly understood. Here, we analyze patterns V. genome across various cell cycle stages. We find that primarily binds sites inhibiting initiation at Chr2 origin ( ori2 ). This inhibitory effect counteracted when replicated Chr1, causing shift...
ANATOMIX is a 200-m-long undulator beamline for full-field tomography techniques at photon energies from 5 to 25 keV. It currently under construction Synchrotron SOLEIL, the French national light source near Paris. will feature experimental stations both parallel-beam microtomography (with beam of up 40 mm width) and zone-plate transmission X-ray microscopy (down pixel sizes 30 nm) in absorption phase contrast. The location on canted straight section SOLEIL storage ring implies specific...
The bacteriophage ΦCD27 is capable of lysing Clostridium difficile, a pathogenic bacterium that major cause for nosocomial infection. A recombinant CD27L endolysin lyses C. difficile in vitro, and represents promising alternative as bactericide. To better understand the lysis mechanism, we have determined crystal structure an autoproteolytic fragment endolysin. covers C-terminal domain endolysin, novel fold identified number lysins target Clostridia bacteria. indicates cleavage occurs at...
Charge-coupled device (CCD)-based X-ray detectors allow data to be collected much more quickly (approximately 10 times) than with current on-line imaging-plate systems. At the ESRF, image intensifier/CCD detector systems have been developed. These great potential as fast read-out for macromolecular and other forms of crystallography. They are relatively large sensitive but two inherent weaknesses: convex detection surfaces leading spatial distortion non-uniformity intensity response,...
An experimental workstation for protein crystallography using synchrotron X-radiation is described. Different modes of the single, bent, triangular monochromator are discussed both rapid collection high Bragg resolution native crystal data and spectral (( delta lambda / )<or approximately=5*10-4) optimised anomalous dispersion studies solution so-called crystallographic phase problem. indication way in which experiment can accommodate small angle work also given.
The structure of the catalytically active, reduced, form enzyme hydroxymethylbilane synthase (HMBS, Lys59Gln mutant) has been studied by Laue diffraction as substrate, porphobilinogen (PBG), was fed to an immobilised crystal in a flow cell. data at short time-scale time points (i.e. 1, 2, 4 and 8 min) were measured using several crystals then averaged. Longer time-point sets 25 min ± 7 2 h 23 9 from individual crystals. All benefited rapid exposures on ESRF ID09 (≈1 ms) fast duty cycle II...
S pili are sialic acid binding hair-like appendages expressed by pathogenic strains of Escherichia coli. The presence has been implicated as a virulence factor in both urinary-tract infections and new-born meningitis. Assembly proceeds via the ubiquitous chaperone/usher pathway. Previously, structures homologous chaperones PapD FimC involved assembly P type-1 pili, respectively, have solved. Here, 2.2 Å X-ray structure pilus chaperone SfaE is reported. same overall L-shaped FimC, with two...
The XPAD3S-CdTe, a CdTe photon-counting pixel array detector, has been used to measure the energy and intensity of white-beam diffraction from lysozyme crystal. A method was developed calibrate detector in terms energy, allowing incident photon measurement high resolution (approximately 140 eV), opening up new possibilities energy-resolved X-ray diffraction. In order demonstrate this, Laue experiments were performed on bending-magnet beamline METROLOGIE at Synchrotron SOLEIL. spectra...
Abstract Bacterial nucleoside monophosphate (NMP) kinases, which convert NMPs to diphosphates (NDP), are investigated as potential antibacterial targets against pathogenic bacteria. Herein, we report the biochemical and structural characterization of GMP kinase from Mycobacterium tuberculosis (GMPK Mt ). GMPK is a monomer with an unusual specificity for ATP phosphate donor, lower catalytic efficiency compared eukaryotic GMPKs, it carries two redox‐sensitive cysteines in central CORE domain....
The histidine kinase (HK) domain belonging to the light–oxygen–voltage (LOV-HK) from Brucella abortus is a member of HWE family, for which no structural information available, and has low sequence identity (20%) closest HK present in PDB. `off-edge' S-SAD method macromolecular X-ray crystallography was used solve structure LOV-HK at resolution crystals low-symmetry space group ( P 2 1 ) with four copies asymmetric unit (∼108 kDa). Data were collected both multiple (diffraction limit varying...