A5087670787- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cardiomyopathy and Myosin Studies
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Photoreceptor and optogenetics research
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Apelin-related biomedical research
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- bioluminescence and chemiluminescence research
- Advanced Electron Microscopy Techniques and Applications
- RNA regulation and disease
- Advanced Biosensing Techniques and Applications
- Viral Infections and Immunology Research
- Protease and Inhibitor Mechanisms
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
- Cellular Mechanics and Interactions
- Characterization and Applications of Magnetic Nanoparticles
Centre National de la Recherche Scientifique
2012-2025
Université Claude Bernard Lyon 1
2024-2025
Laboratoire de Biologie et Modélisation de la Cellule
2019-2025
Lyon College
2024
École Polytechnique
2012-2023
Université Paris-Saclay
2018-2019
Brandeis University
2007-2008
Howard Hughes Medical Institute
2007
Institut Curie
2003-2004
Abstract Archaeal translation initiation occurs within a macromolecular complex containing the small ribosomal subunit (30S) bound to mRNA, factors aIF1, aIF1A and ternary aIF2:GDPNP:Met-tRNA i Met . Here, we determine cryo-EM structure of 30S:mRNA:aIF1A:aIF2:GTP:Met-tRNA from Pyrococcus abyssi at 3.2 Å resolution. It highlights archaeal features in proteins rRNA modifications. We find an aS21 protein, location eS21 eukaryotic ribosomes. Moreover, identify N-terminal extension eL41...
Abstract The nonsense-mediated mRNA decay (NMD) pathway triggers the degradation of defective mRNAs and governs expression with specific characteristics. Current understanding indicates that NMD is often significantly suppressed during viral infections to protect genome. In numerous viruses, this inhibition achieved through direct or indirect interference RNA helicase UPF1, thereby promoting replication enhancing pathogenesis. study, we employed biochemical, biophysical assays cellular...
Abstract In eukaryotes and in archaea late steps of translation initiation involve the two factors e/aIF5B e/aIF1A. eukaryotes, role eIF5B ribosomal subunit joining is established structural data showing bound to full ribosome were obtained. To achieve its function, collaborates with eIF1A. However, illustrating how these interact on small have long been awaited. The archaeal counterparts, aIF5B aIF1A, remains be extensively addressed. Here, we study Pyrococcus abyssi initiation. Using vitro...
The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of genus-specific genes were shown be up-regulated upon exposure radiation and play a role in genome reconstitution. These include SSB-like protein called DdrB. Here, we identified novel encoded by the dr1245 gene as interacting partner strain devoid DR1245 is impaired growth, exhibiting generation time approximately threefold that wild type while radioresistance not affected. We...
Liposomal formulations represent attractive biocompatible and tunable drug delivery systems for peptide drugs. Among the tools to analyze their physicochemical properties, nuclear magnetic resonance (NMR) spectroscopy, despite being an obligatory technique characterize molecular structure dynamics in chemistry as well structural biology, yet appears be rather sparsely used study drug-liposome formulations. In this work, we exploited several facets of liquid-state NMR spectroscopy liposomal...
Abstract ExbB and ExbD are cytoplasmic membrane proteins that associate with TonB to convey the energy of proton-motive force outer receptors in Gram-negative bacteria for iron uptake. The opportunistic pathogen Serratia marcescens ( Sm ) possesses both a heme-specific paralog, HasB. has long periplasmic extension absent other such as E. coli (Ec) . Long ExbB’s found several genera Alphaproteobacteria, most often correlation hasB gene. We investigated specificity determinants determined...
ABSTRACT The archaeal ribosome is of the eukaryotic type. Genomic and phylogenetic studies have indicated that TACK Asgard, closest relatives eukaryotes, ribosomes containing ribosomal proteins not found in other branches, eS25, eS26 eS30. In our study, we investigated case Saccharolobus solfataricus , a crenarchaeon belonging to branch, which mainly uses leaderless mRNAs. We characterized small subunit S. bound SD-leadered or mRNAs (lmRNAs). Cryo-EM structures show for first time versions...
In archaeal translation initiation, a preinitiation complex (PIC) made up of aIF1, aIF1A, the ternary (TC, e/aIF2-GTP-Met-tRNAiMet) and mRNA bound to small ribosomal subunit is responsible for start codon selection. Many mRNAs contain Shine-Dalgarno (SD) sequence allowing PIC be prepositioned in vicinity codon. Nevertheless, cryo-EM studies have suggested local scanning definitely establish base pairing with tRNA anticodon. Here, using fluorescence anisotropy, we show that aIF1 synergistic...
Translation initiation in eukaryotes and archaea involves a methionylated initiator tRNA delivered to the ribosome ternary complex with e/aIF2 GTP. Eukaryotic archaeal tRNAs contain highly conserved A 1 –U 72 base pair at top of acceptor stem. The importance this discriminate from elongator has been established previously using genetics biochemistry. However, no structural data illustrating how participates accurate selection by translation systems are available. Here, we describe crystal...
The Nonsense-mediated mRNA decay (NMD) pathway triggers the degradation of defective mRNAs and governs expression with specific characteristics. Current understanding indicates that NMD is often significantly suppressed during viral infections to protect genome. In numerous viruses, this inhibition achieved through direct or indirect interference RNA helicase UPF1, thereby promoting replication enhancing pathogenesis. study, we employed biochemical, biophysical assays, cellular...
Sample preparation on cryo-EM grids can give various results, from very thin ice and homogeneous particle distribution (ideal case) to unwanted behavior such as particles around the "holes" or complexes that do not entirely correspond one in solution (real life). We recently run into a case finally found out variations 3D reconstructions were systematically correlated with grid batches used. report use of several techniques investigate grids' characteristics, namely TEM, SEM, Auger...
Abstract ExbB and ExbD are cytoplasmic membrane proteins that associate with TonB to convey the energy of proton-motive force outer receptors in Gram-negative bacteria for iron uptake. The opportunistic pathogen Serratia marcescens ( Sm ) possesses both a heme-specific paralog, HasB. has long periplasmic extension absent other such as E. coli (Ec) . Long ExbB’s found several genera Alphaproteobacteria, most often correlation hasB gene. We investigated specificity determinants determined...