A5008724415- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- DNA Repair Mechanisms
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Legume Nitrogen Fixing Symbiosis
- Microtubule and mitosis dynamics
- Methane Hydrates and Related Phenomena
- Radioactive contamination and transfer
- Antibiotic Resistance in Bacteria
- Hereditary Neurological Disorders
- Carcinogens and Genotoxicity Assessment
- Microbial Metabolism and Applications
- RNA and protein synthesis mechanisms
- Yersinia bacterium, plague, ectoparasites research
- Plant Genetic and Mutation Studies
- Molecular Biology Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Effects of Radiation Exposure
Université de Montréal
2024-2025
University of Technology Sydney
2018-2024
University of Freiburg
2021-2024
CEA Paris-Saclay
2015-2023
Université Paris-Saclay
2015-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2023
Institut de Biologie Intégrative de la Cellule
2015-2023
Centre National de la Recherche Scientifique
2015-2023
Université Paris-Sud
2013-2015
Archaeal cell biology is an emerging field expected to identify fundamental cellular processes, help resolve the deep evolutionary history of life, and contribute new components functions in biotechnology synthetic biology. To facilitate these, we have developed plasmid vectors that allow convenient cloning production proteins fusion with flexible, rigid, or semi-rigid linkers model archaeon Haloferax volcanii . For protein subcellular localization studies using fluorescent (FP) tags,...
Deinococcus radiodurans is known for its extreme radioresistance. Comparative genomics identified a radiation-desiccation response (RDR) regulon comprising genes that are highly induced after DNA damage and containing conserved motif (RDRM) upstream of their coding region. We demonstrated the RDRM sequence involved in cis-regulation RDR gene ddrB vivo. Using transposon mutagenesis approach, we showed that, addition to ddrO encoding predicted repressor irrE positive regulator recently shown...
Abstract Archaeal CetZ cytoskeletal proteins are a major group of the tubulin superfamily involved in development motile rod-shaped cells. In model archaeon Haloferax volcanii , it has been unknown whether CetZs contribute to motility solely through effects rod cell shape on swimming speed, or other ways too. Here, we used cryo-electron and fluorescence microscopy observe surface filaments assembly machinery cells carrying cetZ1 cetZ2 deletion, overexpression, dominant GTPase-deficient...
MinD proteins are well studied in rod-shaped bacteria such as E. coli, where they display self-organized pole-to-pole oscillations that important for correct positioning of the Z-ring at mid-cell cell division. Archaea also encode belonging to family, but their functions unknown. homologous were found be widespread Euryarchaeota and form a sister group bacterial distinct from ParA other related ATPase families. We aimed identify function four archaeal model archaeon Haloferax volcanii....
Horizontal gene transfer is a major driver of bacterial evolution and adaptation to environmental stresses, occurring notably via transformation naturally competent organisms. The Deinococcus radiodurans bacterium, characterized by its extreme radioresistance, also competent. Here, we investigated the role D. players involved in different steps natural transformation. First, identified factors (PilQ, PilD, type IV pilins, PilB, PilT, ComEC-ComEA ComF) DNA uptake translocation across external...
Abstract Archaeal cell biology is an emerging field expected to identify fundamental cellular processes, help resolve the deep evolutionary history of life, and contribute new components functions in biotechnology synthetic biology. To facilitate these, we have developed plasmid vectors that allow convenient cloning production proteins fusion with flexible, rigid, or semi-rigid linkers model archaeon Haloferax volcanii . For protein subcellular localization studies using fluorescent (FP)...
In bacteria and archaea, proteins of the ParA/MinD family ATPases regulate spatiotemporal organization various cellular cargoes, including cell division proteins, motility structures, chemotaxis systems, chromosomes. bacteria, such as
The bacterium Deinococcus radiodurans is one of the most radioresistant organisms known. It able to reconstruct a functional genome from hundreds radiation-induced chromosomal fragments. Our work aims highlight genes involved in recombination between 438 bp direct repeats separated by intervening sequences various lengths ranging 1,479 10,500 restore tetA gene presence or absence DNA double strand breaks. frequency spontaneous deletion events were same recA+ and ΔrecA, ΔrecF, ΔrecO bacteria,...
The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of genus-specific genes were shown be up-regulated upon exposure radiation and play a role in genome reconstitution. These include SSB-like protein called DdrB. Here, we identified novel encoded by the dr1245 gene as interacting partner strain devoid DR1245 is impaired growth, exhibiting generation time approximately threefold that wild type while radioresistance not affected. We...
Haloferax volcanii and other Haloarchaea can be pleomorphic, adopting different shapes, which vary with growth stages. Several studies have shown that H. cell shape is sensitive to various external factors including media physical environment. In addition, several noticed the presence of a recombinant plasmid in cells also factor impacting shape, notably by favoring development rods early stages growth. Here we investigated reasons for this phenomenon first studying impact auxotrophic...
The tubulin superfamily of cytoskeletal proteins is widespread in all three domains life — Archaea, Bacteria and Eukarya. Tubulins build the microtubules eukaryotic cytoskeleton, whereas members homologous FtsZ family construct division ring prokaryotes some organelles. Their functions are relatively poorly understood archaea, yet these microbes contain a remarkable diversity proteins, including for division, newly described major called CetZ that involved archaeal cell shape control,...
The tubulin homolog FtsZ assembles a cytokinetic ring in bacteria and plays key role the machinery that constricts to divide cells. Many archaea encode two proteins from distinct families, FtsZ1 FtsZ2, of previously unclear functions. Here we show Haloferax volcanii cannot properly without either or both, but DNA replication continues, cells proliferate alternative ways via remarkable envelope plasticity. FtsZ2 co-localize form dynamic division ring. However, can assemble rings independently...
The extremely radiation and desiccation resistant bacterium Deinococcus radiodurans possesses three genes encoding Endonuclease III-like enzymes (DrEndoIII1, DrEndoIII2, DrEndoIII3). In vitro enzymatic activity measurements revealed that DrEndoIII2 is the main III in this organism, while DrEndoIII1 3 possess unusual and, so far, no detectable EndoIII activity, respectively. order to understand role of these at a cellular level, DrEndoIII knockout mutants were constructed subjected various...
MinD proteins are well studied in rod-shaped bacteria such as E. coli , where they display self-organized pole-to-pole oscillations that important for correct positioning of the Z-ring at mid-cell cell division. Archaea also encode belonging to family, but their functions unknown. homologous were found be widespread Euryarchaeota and form a sister group bacterial distinct from ParA other related ATPase families. We aimed identify function four archaeal model archaeon Haloferax volcanii ....
In bacteria and archaea, proteins of the ParA/MinD family ATPases regulate spatiotemporal organization various cellular cargoes, including cell division proteins, motility structures, chemotaxis systems, chromosomes. bacteria, such as