A5048650129- Neuroscience and Neuropharmacology Research
- Sphingolipid Metabolism and Signaling
- Neurogenesis and neuroplasticity mechanisms
- Lysosomal Storage Disorders Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Selenium in Biological Systems
- Axon Guidance and Neuronal Signaling
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Trace Elements in Health
- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Nerve injury and regeneration
- Carbohydrate Chemistry and Synthesis
- Signaling Pathways in Disease
- Receptor Mechanisms and Signaling
- ATP Synthase and ATPases Research
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Fatty Acid Research and Health
- Organoselenium and organotellurium chemistry
- Autoimmune and Inflammatory Disorders Research
- Dermatology and Skin Diseases
Carl von Ossietzky Universität Oldenburg
2018-2025
University of Rostock
2017-2022
Charité - Universitätsmedizin Berlin
2007-2021
Saarland University
2016
Universitätsmedizin Rostock
2015
Institute of Cell Biology and Neurobiology
2012
University of Veterinary Medicine Vienna
2012
MSB Medical School Berlin
2003-2007
Humboldt-Universität zu Berlin
2000-2006
Philips (Germany)
2004
While idiopathic generalized epilepsies are thought to evolve from temporal highly synchronized oscillations between thalamic and cortical networks, their cellular basis remains poorly understood. Here we show in a genetic rat model of absence epilepsy (WAG/Rij) that rapid decline expression hyperpolarization-activated cyclic-nucleotide gated (HCN1) channels (I(h)) precedes the onset seizures, suggesting loss HCN1 channel is inherited rather than acquired. Loss occurs primarily apical...
Thyroid hormone transport into cells requires plasma membrane proteins. Mutations in one of these, monocarboxylate transporter 8 (MCT8), have been identified as underlying cause for the Allan–Herndon–Dudley syndrome, an X-linked mental retardation which patients also present with abnormally high 3′,3,5-triiodothyronine (T 3 ) levels. Mice deficient Mct8 replicate thyroid abnormalities observed human condition. However, no neurological deficits described mice lacking . Therefore, we subjected...
Abstract Neuronal subthreshold excitability and firing behaviour are markedly influenced by the activation deactivation of somato‐dendritic hyperpolarization‐activated cation current ( I h ). Here, we evaluated possible contributions to hyperexcitability in an animal model absence seizures (WAG/Rij rats). We investigated pyramidal neurons somatosensory neocortex, site generation spike–wave discharges. ‐mediated functions from WAG/Rij rats, Wistar rats (sharing same genetic background with...
Excitotoxic brain lesions, such as stroke and epilepsy, lead to increasing destruction of neurons hours after the insult. The deadly cascade events involves detrimental actions by free radicals activation proapoptotic transcription factors, which finally result in neuronal destruction. Here, we provide direct evidence that nutritionally essential trace element selenium has a pivotal role susceptibility excitotoxic lesions. First, observed cell cultures addition form selenite within...
Cellular thyroid hormone uptake and efflux are mediated by transmembrane transport proteins. One of these, monocarboxylate transporter 8 (MCT8) is mutated in Allan-Herndon-Dudley syndrome, a severe mental retardation associated with abnormal constellations. Since mice deficient Mct8 exhibit milder neurological phenotype than patients, we hypothesized that alternative transporters may compensate murine brain cells for the lack Mct8. Using qPCR, Western Blot, immunocytochemistry, investigated...
The application of radionuclides for the localization essential trace elements in vivo and characterization their binding proteins is a story intermittently made improvements techniques used detection. In this study we present use neutron activation analysis different autoradiographic imaging methods including real-time digital autoradiography to reveal new insights hierarchy selenium homeostasis. Selenoproteins containing element play important roles CNS. Although CNS does not show highest...
Inkjet printing techniques are often used for bioprinting purposes because of their excellent characteristics, such as high cell viability and low apoptotic rate, contactless modus operandi, commercial availability, cost. However, they face some disadvantages, the use bioinks viscosity, damage due to shear stress caused by drop ejection jetting velocity, well a narrow range available that still challenge inkjet technology. New technological solutions required overcome these obstacles....
Abstract Although absence epilepsy has a genetic origin, evidence from an animal model (Wistar Albino Glaxo/Rijswijk; WAG/Rij) suggests that seizures are sensitive to environmental manipulations. Here, we show manipulations of the early rearing environment (neonatal handling, maternal deprivation) WAG/Rij rats leads pronounced decrease in seizure activity later life. Recent observations link hyperpolarization‐activated cation current ( I h ) somatosensory cortex, site generation. Therefore,...
Uncoupling protein 2 (UCP2) is an inner mitochondrial membrane protein. Although the was discovered in 1997, its function and even tissue distribution are still under debate. Here we present a quantitative analysis of mRNA expression various mice tissues, revealing that UCP2 mainly expressed organs cells associated with immune system. gene brain, as demonstrated using RT-PCR, not detectable neurons physiological conditions. Instead, could detect microglia, which act defense central nervous...
Differentiation of immature osteoblasts to mature in vitro initially was induced by supplementing the medium with β-gylcerophosphate and dexamethasone. Later, ascorbic acid, vitamin D3, K3 TGFβ1 were used varying concentrations as supplements generate a osteoblast phenotype. We tested effects several combinations cell culture media, seeding protocols osteogenic on differentiation human primary osteoblasts. Osteogenic analyzed staining alkaline phosphatase (ALP)...
Abstract Background LPA is a small bioactive phospholipid that acts as an extracellular signaling molecule and involved in cellular processes, including cell proliferation, migration, differentiation. by binding activating at least six known G protein–coupled receptors: 1–6 . In recent years, has been suggested to play important role both normal neuronal development under pathological conditions the nervous system. Results We show expression pattern of receptors during mouse brain using...
The Nogo gene encodes an integral membrane protein mainly responsible for the neurite inhibition properties of myelin. Here, we analyzed expression pattern Nogo-A, Nogo-B, and Nogo-C Nogo-66 receptor (Ng66R) mRNA during hippocampal development lesion-induced axonal sprouting. Nogo-A Nogo-B Ng66R transcripts preceded progress myelination were highly expressed at postnatal day zero (P0) in all principal cell layers, with exception dentate granule cells. Only a slight was found P0 layers...
Apart from the first family member, uncoupling protein 1 (UCP1), functions of other UCPs (UCP2-UCP5) are still unknown. In analyzing our own results and those previously published by others, we have assumed that UCP's cellular expression pattern coincides with a specific cell metabolism changes if latter is altered. To verify this hypothesis, analyzed UCP1-5 in mouse embryonic stem cells before after their differentiation to neurons. We shown only UCP2 present undifferentiated it disappears...
Abstract Phospholipid‐mediated signalling on neurons provokes diverse responses such as neurogenesis, pattern formation and neurite remodelling. We have recently uncovered a novel set of molecules in the mammalian brain, named plasticity‐related genes (PRGs), which mediate lipid phosphate phosphatase activity provide evidence for their involvement mechanisms neuronal plasticity. Here, we report new member vertebrate‐specific PRG family, gene‐3 (PRG‐3). PRG‐3 is heavily expressed brain shows...
Differential display of hippocampal tissue after entorhinal cortex lesion (ECL) revealed decreases in mRNA encoding the neuronal hyperpolarization-activated, cyclic nucleotide-gated channel HCN1. In situ hybridization confirmed that transcripts HCN1, but not HCN2/3/4, are down-regulated ECL. Expression recovered at approximately 21 days (dal). Immunohistochemistry demonstrated a corresponding regulation HCN1 protein expression CA1-CA3 dendrites, hilar mossy cells and interneurons, granule...