A5091376342- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Allergic Rhinitis and Sensitization
- Immune Cell Function and Interaction
- Food Allergy and Anaphylaxis Research
- Protein purification and stability
- Immunodeficiency and Autoimmune Disorders
- Asthma and respiratory diseases
- Herpesvirus Infections and Treatments
- Blood groups and transfusion
- Biofuel production and bioconversion
- Carbohydrate Chemistry and Synthesis
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- Polysaccharides and Plant Cell Walls
- SARS-CoV-2 and COVID-19 Research
- Toxoplasma gondii Research Studies
- Chronic Lymphocytic Leukemia Research
- Enzyme Production and Characterization
- HIV Research and Treatment
- Neuroendocrine regulation and behavior
- Galectins and Cancer Biology
- Microfluidic and Bio-sensing Technologies
Lund University
2016-2025
Science for Life Laboratory
2017-2025
Stockholm University
2022
Karolinska Institutet
2022
KTH Royal Institute of Technology
2022
Medicon Village
2013-2021
Skåne University Hospital
2019
Getinge (Sweden)
2019
Alligator Bioscience (Sweden)
2014
BioInvent (Sweden)
1992-1995
Microarrays are powerful tools for high throughput analysis, and hundreds or thousands of molecular interactions can be assessed simultaneously using very small amounts analytes. Nucleotide microarrays well established in plant research, but carbohydrate much less established, one reason this is a lack suitable glycans with which to populate arrays. Polysaccharide relatively easy produce because the ease immobilizing large polymers noncovalently onto variety microarray surfaces, they...
Spike-specific antibodies are central to effective COVID19 immunity. Research efforts have focused on that neutralize the ACE2-Spike interaction but not non-neutralizing antibodies. Antibody-dependent phagocytosis is an immune mechanism enhanced by opsonization, where typically, more bound trigger a stronger phagocyte response. Here, we show antibodies, dependent concentration, can either enhance or reduce Spike-bead monocytes independently of antibody neutralization potential. Surprisingly,...
Analysis of an individual's immunoglobulin (IG) gene repertoire requires the use high-quality germline reference sets. When sets only contain alleles supported by strong evidence, AIRR sequencing (AIRR-seq) data analysis is more accurate and studies evolution IG genes, their allelic variants expressed immune therefore facilitated.
The learning that ensued from the mentorship relationship on a program for doctoral students at Swedish university was studied in three cases (two social science and one technology). aim was: (a) to explore how students, their formal mentors supervisors describe own learning, they perceive of other individuals who are part developmental relationship; (b) students' outcomes. A total nine semi-structured interviews were conducted. results show variations reciprocal among participants, both...
Antibodies play a central role in the immune defense against SARS-CoV-2. Emerging evidence has shown that nonneutralizing antibodies are important for through Fc-mediated effector functions. Antibody subclass is known to affect downstream Fc function. However, whether antibody plays anti-SARS-CoV-2 immunity remains unclear. Here, we subclass-switched eight human IgG1 anti-spike monoclonal (mAbs) IgG3 by exchanging their constant domains. The mAbs exhibited altered avidities spike protein and...
Abstract Streptococcus pyogenes can cause invasive disease with high mortality despite adequate antibiotic treatments. To address this unmet need, we have previously generated an opsonic IgG1 monoclonal antibody, Ab25, targeting the bacterial M protein. Here, engineer IgG2-4 subclasses of Ab25. Despite having reduced binding, IgG3 version promotes stronger phagocytosis bacteria. Using atomic simulations, show that IgG3’s Fc tail has extensive movement in 3D space due to its extended hinge...
The humoral immune response to human cytomegalovirus (CMV) membrane glycoprotein gp58/116 (gB) has been studied by establishing cell lines producing specific monoclonal antibodies. These were generated from peripheral blood lymphocytes obtained a healthy carrier. Hybridomas gp58/116-specific antibodies detected reactivity procaryotically expressed proteins containing the major neutralizing epitopes of this complex. One antibody, ITC88, which recognized an epitope located between amino acid...
Human PBL from vaccinated healthy blood donors, which was transplanted i.p. into mice with severe combined immunodeficiency (SCID), exhibited an Ag-dependent humoral Ir against tetanus toxoid. This dose dependent and completely abrogated by immunizing large amounts of Ag, suggesting a high tolerization the B cells. A dose-dependent selection specific, affinity clonotypes also suggested, since immunization low concentrations toxoid produced antisera higher avidity than immunizations using Ag....
Surface plasmon resonance, i.e. detection of changes in refractive index on a surface, was used biosensor to evaluate the dissociation/association rate and affinity constants human monoclonal IgG IgM antibodies Tab fragments. The results showed that an observed difference between intact fragmented anti‐tetanus antibody related approximately 10‐fold differences dissociation constants, since association were same range, 2–3×10 5 ( m ‐1 s ). Affinity as determined by conventional solid phase...
Abstract Summary Antibody repertoires reveal insights into the biology of adaptive immune system and empower diagnostics therapeutics. There are currently multiple tools available for annotation antibody sequences. All downstream analyses such as choosing lead drug candidates depend on correct these sequences; however, a thorough comparison performance has not been investigated. Here, we benchmark commonly used immunoinformatic tools, i.e. IMGT/HighV-QUEST, IgBLAST MiXCR, in terms...
Abstract High-throughput sequencing of the adaptive immune receptor repertoire (AIRR-seq) is providing unprecedented insights into response to disease and development disorders. The accurate interpretation AIRR-seq data depends on existence comprehensive germline gene reference sets. Current sets are known be incomplete unrepresentative degree polymorphism diversity in human animal populations. A key issue complexity genomic regions which they lie, which, because presence multiple repeats,...
Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides large viruses was realised in a microscaled continuous flow format. The bead principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force an ultrasonic standing wave laminar properties are utilised translate bioanalytes trapped functionalised one carrier fluid another. carry-over non-specific material ranges 1 50 ppm relative input levels depending...
Specific immunotherapy (SIT) is the only treatment with proved long-term curative potential in patients allergic disease. Allergen-specific IgE causative agent of disease, and antibodies contribute to SIT, but effects SIT on aeroallergen-specific B-cell repertoires are not well understood.We sought characterize sequences expressed by allergen-specific B cells track fate these clones during SIT.We used high-throughput antibody gene sequencing identification combinatorial fragment library...
Analysis of antibody repertoire development and specific responses important for e.g. autoimmune conditions, allergy, protection against disease is supported by high throughput sequencing associated bioinformatics pipelines that describe the diversity encoded variable domains. Proper assignment sequences to germline genes are many such processes, instance in analysis somatic hypermutation. Germline gene inference from antibody-encoding transcriptomes, using tools as TIgGER or IgDiscover, has...
Abstract In adaptive immune receptor repertoire analysis, determining the germline variable (V) allele associated with each T- and B-cell sequence is a crucial step. This process highly impacted by annotations. Aligning sequences, assigning them to specific alleles, inferring individual genotypes are challenging when mutated, or reads do not cover whole V region. Here, we propose an alternative naming scheme for as well novel method infer genotypes. We demonstrate strengths of two comparing...