A5050601905- Hippo pathway signaling and YAP/TAZ
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- COVID-19 epidemiological studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- COVID-19 Impact on Reproduction
- Immune cells in cancer
- Wnt/β-catenin signaling in development and cancer
Lund University
2021-2024
Spike-specific antibodies are central to effective COVID19 immunity. Research efforts have focused on that neutralize the ACE2-Spike interaction but not non-neutralizing antibodies. Antibody-dependent phagocytosis is an immune mechanism enhanced by opsonization, where typically, more bound trigger a stronger phagocyte response. Here, we show antibodies, dependent concentration, can either enhance or reduce Spike-bead monocytes independently of antibody neutralization potential. Surprisingly,...
Light microscopy is a powerful single-cell technique that allows for quantitative spatial information at subcellular resolution. However, unlike flow cytometry and sequencing techniques, has issues achieving high-quality population-wide sample characterization while maintaining high Here, we present general framework, data-driven (DDM) uses real-time object to enable high-fidelity imaging of relevant phenotypes based on the population context. DDM combines data-independent data-dependent...
Antibody binding to cell surface proteins plays a crucial role in immunity, and the location of an epitope can altogether determine immunological outcome host-target interaction. Techniques available today for identification are costly, time-consuming, unsuited high-throughput analysis. Fast efficient screening be useful development therapeutic monoclonal antibodies vaccines. Cellular morphology typically varies, often bind heterogeneously across surface, making traditional...
Spike-specific antibodies are central to effective COVID19 immunity. Research efforts have focused on that neutralize the ACE2-Spike interaction but not non-neutralizing antibodies. Antibody-dependent phagocytosis is an immune mechanism enhanced by opsonization, where typically, more bound trigger a stronger phagocyte response. Here, we show antibodies, dependent concentration, can either enhance or reduce Spike-bead monocytes independently of antibody neutralization potential. Surprisingly,...
Neutrophil extracellular traps (NETs) are structures that neutrophils form in response to various stimuli, including invading pathogens. NETs increasingly studied, and their importance has been demonstrated autoimmunity infection. However, no consensus emerged on quantification, with many studies resorting manually counting microscopy images. NETQUANT is a free software for the automated quantification of neutrophil fluorescence By employing image analysis based biologically relevant...
Light microscopy is a powerful single-cell technique that allows for quantitative spatial information at subcellular resolution. However, unlike flow cytometry and sequencing techniques, has issues achieving high-quality population-wide sample characterization while maintaining high Here, we present general framework, data-driven (DDM), uses cell to enable high-fidelity imaging of relevant phenotypes. DDM combines data-independent data-dependent steps synergistically enhance data acquired...