A5055901288- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
- Toxin Mechanisms and Immunotoxins
- Immunotherapy and Immune Responses
- Image Processing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Force Microscopy Techniques and Applications
- Escherichia coli research studies
- CAR-T cell therapy research
Lund University
2020-2023
Article12 December 2022Open Access Transparent process A human monoclonal antibody bivalently binding two different epitopes in streptococcal M protein mediates immune function Wael Bahnan Division of Infection Medicine, Department Clinical Sciences Lund, Faculty Lund University, Sweden Contribution: Conceptualization, Resources, Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing - original draft, review & editing Search for more papers by this...
Group A streptococcus (GAS) is a highly adapted, human-specific pathogen that known to manipulate the immune system through various mechanisms. GAS' M protein constitutes primary target of due its spatial configuration and dominance on bacterial surface. Antibody responses targeting have been shown favor conserved C region. Such antibodies (Abs) circumvent antigenic escape efficiently bind types. The ability GAS fibronectin (Fn), high molecular weight glycoprotein extracellular matrix, has...
Antibody binding to cell surface proteins plays a crucial role in immunity, and the location of an epitope can altogether determine immunological outcome host-target interaction. Techniques available today for identification are costly, time-consuming, unsuited high-throughput analysis. Fast efficient screening be useful development therapeutic monoclonal antibodies vaccines. Cellular morphology typically varies, often bind heterogeneously across surface, making traditional...
Many bacteria can interfere with how antibodies bind to their surfaces. This bacterial antibody targeting makes it challenging predict the immunological function of bacteria-associated antibodies. The M and M-like proteins group A streptococci (GAS) exhibit IgGFc-binding regions, which they use reverse IgG binding orientation depending on host environment. Unraveling mechanism behind these characteristics may identify conditions under bound drive an efficient immune response. Here, we have...
Group A streptococci have evolved multiple strategies to evade human antibodies, making it challenging create effective vaccines or antibody treatments. Here, we generated antibodies derived from the memory B cells of an individual who had successfully cleared a group streptococcal infection. The bind with high affinity in central region surface-bound M protein. Such are typically non-opsonic. However, one could effectively promote vital immune functions, including phagocytosis and vivo...
The human pathogen Streptococcus pyogenes causes substantial morbidity and mortality. It is unclear if antibodies developed after infections with this are opsonic they strain specific or more broadly protective. Here, we quantified the opsonic-antibody response following invasive S. infection. Four patients bacteremia between 2018 2020 at Skåne University Hospital in Lund, Sweden, were prospectively enrolled. Acute- convalescent-phase sera obtained, isolates genome sequenced (emm118, emm85,...
Many bacteria can interfere with how antibodies bind to their surfaces. This bacterial antibody targeting makes it challenging predict the immunological function of bacteria-associated antibodies. The M and M-like proteins group A streptococci exhibit IgGFc-binding regions, which they use reverse IgG binding orientation depending on host environment. Unraveling mechanism behind these characteristics may identify conditions under bound drive an efficient immune response. Here, we have...
Antibody binding to cell surface proteins plays a crucial role in immunity and the location of an epitope can altogether determine immunological outcome host-target interaction. Techniques available today for identification are costly, time-consuming, unsuited high-throughput analysis. Fast efficient screening be useful development therapeutic monoclonal antibodies vaccines. In present work, we have developed method imaging-based localization sites on cellular proteins. The morphology...
Group A streptococcus (GAS) is a highly adapted, humanspecific pathogen that known to manipulate the immune system through various mechanisms. GAS’ M protein constitutes primary target of due its spatial configuration and dominance on bacterial surface. Antibody responses targeting have been shown favor conserved C region. Such antibodies circumvent antigenic escape efficiently bind types. The ability GAS fibronectin (Fn), high molecular weight glycoprotein extracellular matrix, has long be...
Abstract Introduction The human pathogen Streptococcus pyogenes causes substantial morbidity and mortality. It is unclear if antibodies developed after infections with this are opsonic they strain-specific or more broadly protective. Here, we quantified the antibody response following invasive S. infection. Materials Methods Four patients bacteremia between 2018-2020 at Skåne University Hospital in Lund, Sweden, were prospectively enrolled. Acute convalescent sera obtained, isolates...