A5064494428- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- vaccines and immunoinformatics approaches
- Inflammatory Bowel Disease
- IL-33, ST2, and ILC Pathways
- RNA Interference and Gene Delivery
- Hematopoietic Stem Cell Transplantation
- Galectins and Cancer Biology
- Immune Response and Inflammation
- Microscopic Colitis
- Gut microbiota and health
- Mesenchymal stem cell research
- CAR-T cell therapy research
- Bariatric Surgery and Outcomes
- Psoriasis: Treatment and Pathogenesis
- Gestational Diabetes Research and Management
- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Obesity and Health Practices
- Neuroinflammation and Neurodegeneration Mechanisms
- Gastrointestinal motility and disorders
University of Copenhagen
2013-2024
Nerviano Medical Sciences
2024
Merck (Germany)
2020
Merck (Japan)
2019
Copenhagen University Hospital
2015
Bispebjerg Hospital
2009
Roskilde Sygehus
2006
Rigshospitalet
1994
Cancer-associated autoantibodies hold promise as sensitive biomarkers for early detection of cancer. Aberrant post-translational variants proteins are likely to induce autoantibodies, and changes in O-linked glycosylation represent one the most important cancer-associated modifications (PTMs). Short aberrant O-glycans on may introduce novel glycopeptide epitopes that can elicit because lack tolerance. Technical barriers, however, have hampered such glycopeptide-specific autoantibodies. Here,...
Flow cytometry is now accepted as an ideal technology to reveal changes in immune cell composition and function. However, it also error-prone variable technology, which makes difficult reproduce findings across laboratories. We have recently developed a strategy standardize whole blood flow cytometry. The performance of our protocols was challenged here by profiling samples from healthy volunteers age- gender-dependent differences establish standardized reference cohort for use clinical...
T helper (TH) 17 cells are believed to play a pivotal role in development of inflammatory bowel disease, and their contribution intestinal inflammation has been studied various models colitis. TH17 produce range cytokines, some which potential targets for immunotherapy. However, blockade IL-17A alone with secukinumab was not effective Crohn's disease. In this regard, the pathogenic impact versus IL-17F during is still unresolved.Development IFN-γ-producing, IL-17A-producing, IL-17F-producing...
Background It has been established that patients with chronic rhinosinusitis nasal polyps (CRSwNP) often have co-existing asthma. Objective We aimed to test two hypotheses: (i) upper and lower airway inflammation in CRSwNP is uniform agreement the united airways concept; (ii) bronchial exists all irrespective of clinical asthma status. Methods collected biopsies from polyps, inferior turbinates bronchi 27 6 controls. All participants were evaluated for disease according international...
Therapeutic dendritic cell (DC) vaccination against cancer is a strategy aimed at activating the immune system to recognize and destroy tumor cells. In this nonrandomized phase 1/2 trial, we investigated safety, feasibility, induction of T-cell response, clinical response after treatment with DC-based vaccine in patients metastatic renal carcinoma. Twenty-seven progressive cytokine-refractory carcinoma were vaccinated DCs loaded either cocktail survivin telomerase peptides or lysate...
Recent reports from cancer screening trials in high-risk populations suggest that autoantibodies can be detected before clinical diagnosis. However, there is minimal data on the role of autoantibody signatures general population. Informative p53 peptides were identified sera patients with colorectal using an microarray 15-mer overlapping covering complete sequence. The selected evaluated a blinded case–control study stored serum multimodal arm United Kingdom Collaborative Trial Ovarian...
microRNAs (miRNAs) are small noncoding RNAs that guide degradation of mRNA and regulate protein expression. miRNA based diagnostic biomarkers for ulcerative colitis (UC) Crohn's disease (CD) emerging but information about the cellular localization many miRNAs is limited more detailed histologic evaluation expression patterns needed to understand their immunobiological function. Formalin-fixed paraffin-embedded colon biopsies from 10 patients with UC 8 CD together 9 controls were examined by...
To investigate the potential to induce additional cytotoxic T-lymphocyte (CTL) immunity during chronic HIV-1 infection.We selected infrequently targeted or subdominant but conserved HLA-A*0201-binding epitopes in Gag, Pol, Env, Vpu and Vif. These relatively immune silent were modified as anchor-optimized peptides improve immunogenicity delivered on autologous monocyte-derived dendritic cells (MDDCs).Twelve treatment-naïve HLA-A*0201 HIV-1-infected Danish individuals received 1 x 10 MDDCs...
Aberrant glycosylation of mucins and other extracellular proteins is an important event in carcinogenesis the resulting cancer associated glycans have been suggested as targets immunotherapy. We assessed role O-linked GalNAc on antigen uptake, processing, presentation MHC class I II molecules. The effect O-glycosylation was monitored with a model system based ovalbumin (OVA)-MUC1 fusion peptides (+/− glycosylation) loaded onto dendritic cells co-cultured IL-2 secreting OVA peptide-specific T...
Membrane bound mucins are up-regulated and aberrantly glycosylated during malignant transformation in many cancer cells. This results a negatively charged glycoprotein coat which may protect cells from immune surveillance. However, only limited data have so far demonstrated the critical steps glycan elongation that make affect interaction between cytotoxic effector of system. Tn (GalNAc-Ser/Thr), STn (NeuAcα2-6GalNAc-Ser/Thr), T (Galβ1-3GalNAc-Ser/Thr), ST (NeuAcα2-6Galβ1-3GalNAc-Ser/Thr)...
Summary Chronic inflammation may drive development of cancer as observed in inflammation‐induced colorectal (CRC). Though immune cells can infiltrate the tumour microenvironment, seem to evade anti‐tumour responses, which is one established hallmarks cancer. Targeting programmed cell death protein‐1 (PD‐1)/PD‐L1 signalling pathway currently at forefront immunity‐based therapies for multiple malignancies. By blocking immune‐checkpoint activated T‐cells, it possible rewire adaptive resistance...
Maternal obesity is associated with adverse pregnancy outcomes. Probiotic supplementation during may have positive effects on blood glucose, gestational weight gain (GWG), and the risk of diabetes mellitus [GDM glycated hemoglobin (HbA1c)]. This feasibility study involved a daily probiotic intervention in obese pregnant women from early second trimester until delivery. The primary aim was to investigate effect GWG maternal glucose homeostasis (GDM HbA1c). Secondary aims were infant birth...
Maternal obesity is associated with increased risks of adverse pregnancy-related complications and outcomes for both mothers infants. Overweight obese women have an risk pregnancy-induced hypertension, preeclampsia gestational diabetes mellitus (GDM). Infant Body Mass index (BMI) the in adulthood are related to maternal weight gain (GWG). Preventive lifestyle dietary interventions time-consuming do not always reduce GWG or pregnancy complications. Recent research has indicated that gut...
Abstract Dendritic cells (DC) are promising candidates for cancer immunotherapy. However, it is not known whether in vitro ‐generated monocyte‐derived DC from patients altered compared with healthy donors. In a clinical phase I/II study, were generated utilizing granulocyte macrophage colony‐stimulating factor and rh‐interleukin‐4 (IL‐4) used this we tested the effect of various maturation cocktails performed comparative evaluation phenotype functional characteristics. Polyriboinosinic...