A5006082463- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Parkinson's Disease Mechanisms and Treatments
- Pluripotent Stem Cells Research
- Advanced Neuroimaging Techniques and Applications
- Alzheimer's disease research and treatments
- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- Neurological disorders and treatments
- Muscle Physiology and Disorders
- Periodontal Regeneration and Treatments
- Cardiac Structural Anomalies and Repair
- Neuroscience and Neuropharmacology Research
- Cardiac Fibrosis and Remodeling
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- Cell Adhesion Molecules Research
- Advanced MRI Techniques and Applications
- Cardiovascular Function and Risk Factors
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Sarcoma Diagnosis and Treatment
- Head and Neck Cancer Studies
- Peptidase Inhibition and Analysis
Copenhagen University Hospital
2014-2024
Rigshospitalet
2014-2024
University of Copenhagen
2016-2024
Aalborg University
2014
Abstract The present first-in-human clinical trial evaluated the safety and feasibility of a newly developed cryopreserved Cardiology Stem Cell Centre adipose-derived stromal cell (CSCC_ASC) product from healthy donors for intramyocardial injection in ten patients with ischemic heart disease failure (IHF). Batches CSCC_ASC were isolated three by liposuction abdominal adipose tissue. Adipose mesenchymal cells culture expanded bioreactors without use animal constituents, cryopreserved, stored...
The aim of the SCIENCE trial was to investigate whether a single treatment with direct intramyocardial injections adipose tissue-derived mesenchymal stromal cells (CSCC_ASCs) safe and improved cardiac function in patients chronic ischaemic heart failure reduced ejection fraction (HFrEF).The study European multicentre, double-blind, placebo-controlled phase II using allogeneic CSCC_ASCs from healthy donors or placebo (2:1 randomization). Main inclusion criteria were New York Heart Association...
Abstract Aims Patients suffering from chronic ischaemic heart failure with reduced left ventricular ejection fraction (HFrEF) have quality‐of‐life, repetitive hospital admissions, and life expectancy. Allogeneic cell therapy is currently investigated as a potential treatment option after initially encouraging results clinical autologous allogeneic trials in patients HFrEF. We aimed to investigate the Cardiology Stem Cell Centre Adipose tissue derived mesenchymal Stromal product (CSCC_ASC) an...
Adipose derived stromal cells (ASCs) are a rich and convenient source of for clinical regenerative therapeutic approaches. However, applications ASCs often require cell expansion to reach the needed dose. In this study, cultivation from vascular fraction (SVF) over two passages in automated functionally closed Quantum Cell Expansion System (Quantum system) is compared with traditional manual cultivation. Stromal was isolated abdominal fat, suspended α-MEM supplemented 10% Fetal Bovine Serum...
In vitro expanded adipose-derived stromal cells (ASCs) are a useful resource for tissue regeneration. Translation of small-scale autologous cell production into large-scale, allogeneic process clinical applications necessitates well-chosen raw materials and culture platform. We compare the use clinical-grade human platelet lysate (hPL) fetal bovine serum (FBS) as growth supplements ASC expansion in automated, closed hollow fibre quantum system (bioreactor). Stromal vascular fractions were...
The utility of mesenchymal stromal cells (MSCs) in therapeutic applications for regenerative medicine has gained much attention. Clinical translation MSC-based approaches requires vitro culture-expansion to achieve a sufficient number cells. ideal cell culture medium should be devoid any animal derived components. We have evaluated whether human Platelet Lysate (hPL) could an attractive alternative supplements.MSCs from bone marrow (BMSCs) and adipose tissue-derived (ASCs) obtained three...
Stimulation of mesenchymal stromal cells and adipose tissue-derived (ASCs) with vascular endothelial growth factor (VEGF) has been used in multiple animal studies clinical trials for regenerative purposes. VEGF stimulation is believed to promote angiogenesis usually performed under serum deprivation. Potential molecular mechanisms are numerous the role contributing factors uncertain. The aim current study was investigate effect vitro deprivation on gene expression patterns ASCs.Gene...
Background aims. Adipose-derived stromal cells (ASCs) are attractive sources for cell-based therapies. The hypoxic niche of ASCs in vivo implies that will benefit from hypoxia during vitro expansion. Human platelet lysate (hPL) enhances ASC proliferation rates, compared with fetal bovine serum (FBS) at normoxia. However, the low rates FBS-expanded could be signs senescence or quiescence. We aimed to determine effects and hPL on expansion whether senescent quiescent. Methods. expanded FBS...
Parkinson's disease (PD) is caused by progressive neurodegeneration and characterised motor dysfunction. Neurodegeneration of dopaminergic neurons also causes aberrations within the cortico-striato-thalamo-cortical (CSTC) circuit, which has been hypothesised to lead non-motor symptoms such as depression. Individuals with PD have both lower synaptic density changes in neuronal metabolic function basal ganglia, measured using [11C]UCB-J [18F]FDG positron emission tomography (PET),...
Allogeneic stem cell therapy is more logistically suitable compared with autologous for large-scale patient treatment. We aim to investigate the clinical safety and efficacy profile of allogeneic adipose tissue derived mesenchymal stromal product (CSCC_ASC) as an add-on in patients chronic non-ischaemic heart failure reduced left ventricular ejection fraction (HFrEF) < 40%.
The expeditious progress of Mesenchymal Stromal Cells (MSC) for therapeutic intervention calls means to compare differences in potency cell products. may be attributed innumerable sources including tissue origin, production methods, or even between batches. While the immunomodulatory potential MSC is recognized and well-documented by an expansive body evidence, methodologies findings vary markedly. In this study, we utilized flowcytometric analysis lymphocyte proliferation based on...
Cell therapy for heart disease has been proven safe and efficacious, despite poor cell retention in the injected area. Improving is hypothesized to increase treatment effect. In present study, human adipose-derived stromal cells (ASCs) were delivered an situ forming alginate hydrogel following acute myocardial infarction (AMI) rats. ASCs transduced with luciferase tested ASC phenotype. AMI was inducted nude rats, subsequent injection of saline (controls), 1 × 106 or 1% (w/v) hydrogel....
A positron emission tomography (PET) radiotracer to neuroimage α-synuclein aggregates would be a crucial addition for early diagnosis and treatment development in disorders such as Parkinson's disease, where elevated aggregate levels are histopathological hallmark. The (d3)-[11C]MODAG-001 has recently shown promise visualization of pre-formed fibrils (α-PFF) rodents. We here test the pig model proteins intracerebrally injected immediately before scanning. Four pigs were one hemisphere with...
Long bones and mandibles of 7-weeks-old dy/dy mutant mice their normal littermates were compared. Besides marked differences in length the between two groups, small shape long also observed, whereas present mandibular shape. By using a baseline situated part mandible, considered to be relatively unaffected by muscular dystrophy from which suffer, it was shown that processes mandible becomes markedly affected. However, affection seems differential as coronoid process is less affected than...
Angiogenesis is considered integral to the reparative process after ischemic injury. The αvβ₃ integrin a critical modulator of angiogenesis and highly expressed in activated endothelial cells. 68Ga-NODAGA-E[(cRGDyK)]₂ (RGD) positron-emission-tomography (PET) ligand targeted towards integrin. aim was present data for uptake RGD correlate it with histology further illustrate differences due porcine adipose-derived mesenchymal stromal cell (pASC) or saline treatment minipigs induction...
Abstract Background Adipose-derived stromal cells (ASCs) possess a multitude of regenerative capabilities, which include immunomodulation, angiogenesis, and stimulation extracellular matrix (ECM) remodeling. However, the underlying mechanisms leading to ECM remodeling remain largely elusive highlight need for functional in vitro models mode action studies. Therefore, purpose this study was develop an co-culture model investigate capabilities ASCs modulate fibroblasts ECM. Methods An with...
For cell-based assays studying monocytes and macrophages, the immortalized monocyte cell line THP-1 is widely used stimulated with phorbol 12-myristate 13-acetate, lipopolysaccharide (LPS) and/or interferon-γ (IFN-γ), after which it differentiates polarizes into proinflammatory M1-like macrophages. quantification of this effect different factors affecting these processes, expression levels various maturation markers are determined using reverse transcription-quantitative PCR. purpose, stably...
Investigational cell-based therapeutics are rapidly heading towards pivotal clinical trials. The premise is that the scientific rationale well defined, and product quality reflects exactly this. In vitro potency assays necessary tools for evaluating cell products, with comes high demands standardization reproducibility of methods involved. For demonstrating principles allogeneic use or claimed immunosuppressive efficacies, involving peripheral blood mononuclear cells (PBMC) critical....
Allogeneic cell-based therapies using adipose tissue-derived stromal cells (ASCs) offer an off-the-shelf alternative to autologous therapy. An underlying assumption is that ASC can modulate the immune response of recipient. However, in vitro models are required explore and identify cell interactions mechanisms action, ensure sufficient sustained effects, document these. In this study, we shed light on effect manufactured for clinical use monocyte-derived dendritic inflammatory...
Hyposalivation and xerostomia (dry mouth), are the leading site-effects to treatment of head neck cancer. Currently, there no effective therapies alleviate radiation-induced hyposalivation. Adipose tissue-derived mesenchymal stem/stromal cells (AT-MSCs) have shown potential for restoring salivary gland function. However, mode action is unknown. The purpose present study was therefore characterize effect AT-MSC therapy on proteome in previously irradiated cancer patients.Whole saliva...
Mesenchymal stromal cells have proven capable of improving cardiac pump function in patients with chronic heart failure, yet little is known about their mode action. The aim the study was to investigate short-term effect cryopreserved allogeneic rat adipose tissue-derived (ASC) on composition, cellular subpopulations, and gene transcription a model ischemic cardiomyopathy (ICM). Myocardial infarction (MI) induced by permanent ligation left anterior descending coronary artery. After 6 weeks,...
An increasing number of patients are living with chronic ischemic cardiomyopathy (ICM) and/or heart failure. Treatment options and prognostic tools lacking for many these patients. Our aim was to investigate the value imaging angiogenesis macrophage activation via positron emission tomography (PET) in terms functional improvement after cell therapy. Myocardial infarction induced rats. Animals were scanned [18F]FDG PET echocardiography four weeks randomized allogeneic adipose tissue-derived...