A5051053515- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- Wound Healing and Treatments
- Osteoarthritis Treatment and Mechanisms
- 3D Printing in Biomedical Research
- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Muscle Physiology and Disorders
- Ocular Surface and Contact Lens
- Bone Tissue Engineering Materials
- Problem and Project Based Learning
- Cancer-related molecular mechanisms research
- Periodontal Regeneration and Treatments
- High Altitude and Hypoxia
- Tendon Structure and Treatment
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Renal and related cancers
- Reproductive System and Pregnancy
- RNA Research and Splicing
Aalborg University
2016-2025
Oslo University Hospital
2024
University of Oslo
2024
Klinikum Oldenburg
2018
Institute for Biomedicine
2010
Helios Dr. Horst Schmidt Kliniken Wiesbaden
2006
In-Q-Tel
2005
Danish Cancer Society
1999-2002
Karolinska Institutet
2002
University of Leicester
1995
Human mesenchymal stem cells (hMSCs) have the capacity to differentiate along several pathways form bone, cartilage, tendon, muscle, and adipose tissues. The adult hMSCs reside in vivo bone marrow niches where oxygen concentration is far below ambient air, which most commonly encountered laboratory condition. study reported here was designed determine whether has a role differentiation of into adipocytes. Indeed, when exposed atmosphere containing only 1% oxygen, formation adipocyte-like...
For the accurate determination of gene expression changes during growth and differentiation studies on adipose-derived stem cells (ASCs), quantitative real-time RT-PCR has become a method choice. The technology is very sensitive, however, without proper selection reference genes, to which genes interest are normalized, erroneous results may be obtained. In this study, we have compared levels panel twelve widely used hypoxic culture, osteogenic chondrogenic differentiation, passaging primary...
Abstract Objective: The maintenance of pluripotency human embryonic stem cells (hESCs) requires a high efficiency self‐renewal. During in vitro propagation, however, hESCs have propensity to differentiate spontaneously. In this study, we assessed the nature hESC responses hypoxic conditions. Materials and methods: Human were grown normoxic conditions, expressing Oct4 stage‐specific antigen‐1 identified by indirect immunofluorescence. transcriptional expression Nanog, Notch1, was determined...
Treatment of myocardial infarction (MI) with bone marrow-derived mesenchymal stem cells and recently also adipose-derived has shown promising results. In contrast to clinical trials their use autologous from the ischemic patient, animal MI models are often using young donors young, immune-compromised, recipient animals. Our objective was compare an elderly patient in treatment a fully grown non-immune-compromised rat model. Mesenchymal were isolated adipose tissue marrow compared respect...
Abstract Background Adipose-derived stem cells (ASCs) are being increasingly recognized for their potential to promote tissue regeneration and wound healing. These effects appear be partly mediated by paracrine signaling pathways, enhanced during hypoxia. Mass spectrometry (MS) is a valuable tool proteomic profiling of cultured ASCs, which may help reveal the identity factors secreted under different conditions. However, serum starvation essentially required obtain samples compatible with...
Abstract Adipose-derived stem cells (ASCs) are being tested in clinical trials related to cell-based regenerative therapies. Although most of the current expansion protocols for ASCs use fetal calf serum (FCS), xenogeneic-free medium supplements greatly desired. This study aims compare effect FCS, human platelet lysate (hPL), and a fully defined on initiation maintenance ASC cultures. obtained from five donors were cultured different media: StemPro, Dulbecco's modified Eagle's (DMEM)...
We studied interactions between polar bear peroxisome proliferator-activated receptor gamma (pbPPARG) and selected compounds using a luciferase reporter assay predictions through molecular docking. Furthermore, we adipogenesis by liver adipose tissue extracts from three synthetic mixtures of contaminants in murine 3T3-L1 preadipocytes tissue-derived stem cells (pbASCs). PCB153 p,p'-DDE antagonized pbPPARG, although their predicted receptor-ligand affinity was weak. PBDEs, tetrabromobisphenol...
This study explores first-year university students’ perceptions of psychological safety, team creativity, and academic well-being in problem-based learning (PBL) teams at Aalborg University, where PBL is systematically implemented. These constructs are vital for fostering effective collaboration positive outcomes, yet their interrelations educational contexts remain underexplored. A survey was administered to 893 students across STEM Health programs, using validated instruments well-being....
Abstract In a mouse model, we have shown previously that macrophages are the principal source of complement C1q. Furthermore, spleen, heart, and brain were found to contain substantial levels murine C1q-specific mRNA, whereas liver, kidney, lung, small intestine contained only trace amounts mRNA. This work addresses identification C1q-expressing spleen cells in rat, using Northern blotting situ detection rat C1q mRNA combined with immunohistochemical analysis. The complete sequence encoding...
Background: Before the potential of adipose tissue-derived stem cells can fully be exploited for a broad scope tissue-engineering and cell-based therapeutical applications, an effective reproducible method isolation is needed. Aim: To comparatively analyze five highly defined protease formulations, Blendzyme 1–4, liberase H1 crude collagenase mixture in course digestion that consisted three 1-h intervals. Methods: The resulting digests human tissue aspirates were evaluated yield nucleated...
Development and implementation of therapeutic protocols based on stem cells or tissue-engineered products relies methods that enable the production substantial numbers while complying with stringent quality safety demands. In current study, we aimed to assess benefits maintaining cultures adipose-derived (ASCs) in a defined culture system devoid xenogeneic components (xeno-free) hypoxia over 49-day growth period. Our data provide evidence conditions involving StemPro mesenchymal serum-free...