The role of Mycoplasma genitalium in the pathogenesis pelvic inflammatory disease has not been characterized.Sera from 308 infertile women were investigated for antibodies to M. by immunoblotting. Women with tubal factor infertility (TFI) made up 132 patients, 67 had an male partner and 109 unknown reasons.Of TFI patients 29 (22.0%) seropositive major adhesin, MgPa, versus 11 (6.3%) group normal tubes. No cross-reactions between MgPa P1 related pneumoniae found. Besides, positive sera...

Chlamydia trachomatis infection is associated with severe Fallopian tube tissue damage leading to tubal infertility and ectopic pregnancy. To explore the molecular mechanisms behind an ex vivo model was established from human tubes examined by scanning electron microscopy immunohistochemistry. Extensive destruction affecting especially ciliated cells observed in C. infected organ culture. Interleukin-1 (IL-1) produced epithelial detected after infection. Addition of IL-1 receptor antagonist...

BackgroundThe etiology of the inflammatory bowel diseases, including ulcerative colitis (UC), remains incompletely explained. We hypothesized that an analysis UC colon proteome could reveal novel insights into disease etiology.

The brain vascular basement membrane is important for both blood-brain barrier (BBB) development, stability, and integrity the contribution hereto from capillary endothelial cells (BCECs), pericytes, astrocytes of BBB probably significant. aim this study was to analyse four different in vitro models murine expression possible secretion major proteins BCECs (mBCECs). mBCECs, pericytes glial (mainly microglia) were prepared brains C57BL/6 mice. mBCECs grown as monoculture, co-culture with or...

Synovial fluid in an articulating joint contains proteins derived from the blood plasma and that are produced by cells within tissues, such as synovium, cartilage, ligament, meniscus. The proteome composition of healthy synovial cellular origins many components not fully understood. Here, we present a normative proteomics study using porcine fluid. Using our optimized method, identified 267 with high confidence We also evaluated mRNA expression data tissues can contribute to proteome,...

Abstract Background Adipose-derived stem cells (ASCs) are being increasingly recognized for their potential to promote tissue regeneration and wound healing. These effects appear be partly mediated by paracrine signaling pathways, enhanced during hypoxia. Mass spectrometry (MS) is a valuable tool proteomic profiling of cultured ASCs, which may help reveal the identity factors secreted under different conditions. However, serum starvation essentially required obtain samples compatible with...

BACKGROUND: Mycoplasma genitalium causes urogenital diseases in men and women is presumed to be sexually transmitted. We wanted investigate whether spermatozoa could serve as vectors for M.genitalium order cause upper genital women. METHODS: By use of Nomarski light microscopy transmission X‐ray microscopy, the attachment was studied. Semen incubated vitro with M.genitalium. Purified, motile were examined by immunofluorescence microscopy. RESULTS: shown adhere head, midpiece tail...

The host cell cytoskeleton is known to play a vital role in the life cycles of several pathogenic intracellular microorganisms by providing basis for successful invasion and promoting movement pathogen once inside cytoplasm. McCoy cells infected with Chlamydia trachomatis serovars E or L2 revealed, indirect immunofluorescence microscopy, collocation microtubules ‐containing vesicles during process migration from surface perinuclear location. vast majority microtubule‐associated also...

Chlamydia pneumoniaeis an obligate intracellular human pathogen that causes acute and chronic respiratory tract diseases has been implicated as a possible risk factor in the development of atherosclerotic heart disease. C. pneumoniaecultivated Hep-2 cells were 35S-labeled infectious elementary bodies (EB) purified. The EB proteins separated by two-dimensional gel electrophoresis. Excised protein spots in-gel digested with trypsin peptides concentrated on reverse-phase chromatographic beads...

A polymerase chain reaction (PCR) assay was developed for detection of Chlamydia trachomatis DNA. From the published sequence common C. plasmid, two primer sets were selected. Detection amplified sequences done by agarose gel electrophoresis cleaved or uncleaved sequences, Southern hybridization, dot blot analysis. The PCR optimized and, after 40 cycles amplification with set II, demonstrated a sensitivity 10(-17) g DNA, which corresponds to one copy plasmid. Because high sensitivity, we...

BACKGROUND: To evaluate the role of Chlamydia trachomatis-induced humoral and cell-mediated immune (CMI) responses in predicting tubal factor infertility (TFI). METHODS: Blood samples were taken from 88 women with TFI 163 control women. C. trachomatis chlamydial heat shock protein 60 (CHSP60)-specific immunoglobulin G (IgG) antibodies analysed using enzyme-linked immunosorbent assay (ELISA) kits. Proliferative reactivity peripheral blood mononuclear cells was studied vitro against elementary...

The levels of subclass-specific immunoglobulin G (IgG) antibodies to five microbial antigens in normal human sera were measured by enzyme-linked immunosorbent assays (ELISA). aim was compare the IgG subclass profiles specific diverse originating from virus, intra- and extracellular bacteria. Serum samples 162 Danish women analyzed for IgG1, IgG2, IgG3, IgG4 against a peptide major outer membrane protein (MOMP) Chlamydia trachomatis, native proteins pneumoniae, recombinant Mycoplasma...

Rheumatoid arthritis (RA) is an inflammatory joint disease leading to cartilage damage and ultimately impaired function. To gain new insight into the systemic immune manifestations of RA, we characterized colon mucosa proteome from 11 RA-patients 10 healthy controls. The biopsies were extracted by colonoscopy analyzed label-free quantitative proteomics, enabling quantitation 5366 proteins. abundance dihydrofolate reductase (DHFR) was statistically significantly increased in RA-patient...

Large biobanks exist worldwide containing formalin-fixed, paraffin-embedded samples and stored in RNAlater. However, the impact of tissue preservation on result a quantative proteome analysis remains poorly described. Human colon mucosal biopsies were extracted from sigmoideum either immediately frozen, stabilized RNAlater, or by formalin-fixation. In one set biopsies, formalin stabilization was delayed for 30 min. The protein content characterized high throughput quantitative proteomics. We...

Chlamydia trachomatis L2 is an obligate intracellular microorganism with a unique biphasic life cycle. The extracellular form, the elementary body (EB), infectious but metabolically inactive. Attachment of EBs to host cells medicated by heparan sulfate-like glycosaminoglycan. Following attachment, EB internalized within membrane-bound vesicle, and during first 8 h infection vesicles are transported perinuclear location where they aggregate fuse. By use monoclonal antibody against...

Summary A 27kDa Chlamydia trachomatis L2 protein was characterized by the use of monoclonal antibodies and two‐dimensional gel electrophoresis. The shown to be located in membrane reticulate bodies as well elementary bodies. Its synthesis could detected from 10 hours post‐infection. Cloning sequence analysis distal part gene revealed an open reading frame 175 amino acids. Comparison deduced acid with NBRF data base significant homology between chlamydial product macrophage infectivity...

ABSTRACT Mycoplasma genitalium is known to cause nonchlamydial, nongonococcal urethritis in men and be associated with pelvic inflammatory disease women. Specific sensitive PCR methods are needed for diagnosis of this bacterium because it very difficult culture from patient samples. To determine the bacterial load patients' specimens, a quantitative real-time LightCycler was developed. The housekeeping gene gap encoding glyceraldehyde-3-phosphate dehydrogenase chosen as target gene. assay...

Purified elementary bodies (EBs) of Chlamydia trachomatis serovar L2 were analyzed by chemical cross-linking with disuccinimidyl selenodipropionate. The effect the was immunoblotting sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated components which reacted monoclonal antibodies against major outer membrane protein (MOMP) and lipopolysaccharide (LPS). It shown that in EBs, MOMP cross-linked to LPS component membrane. Migration analysis showed extensive cross-linking, most...

Female infertility can be caused by scarring and occlusion of the Fallopian tubes. Sexually transmitted bacteria damage delicate epithelial layer human tubes (HFT). Genital mycoplasmas are associated with reproductive failure. Yet, there is not enough evidence that cause tubal factor infertility. We analysed effects infections Mycoplasma hominis genitalium on HFT epithelium compared them genital pathogens: Chlamydia trachomatis Neisseria gonorrhoeae. used an in vitro model which pieces...

Serum antibodies against major outer membrane protein (MOMP) and heat shock 60 (HSP60) from Chlamydia trachomatis are correlated with sequelae following infection. Since bacterial human HSP60 share considerable sequence homology, cross-reactivity to is suggested as being involved in tubal factor infertility (TFI). The aim was investigate whether associated TFI, evaluate antibody testing TFI diagnosis.Serum levels of chlamydial MOMP C. trachomatis, Salmonella enterica Enteritidis,...

The aetiologies and pathogeneses of the joint diseases rheumatoid arthritis (RA) spondyloarthritis (SpA) are still not fully elucidated. To increase our understanding molecular pathogenesis, we analysed protein composition synovial fluid (SF) from patients.Fifty-six samples (RA, n = 32; SpA, 24) were digested with trypsin, resulting peptides separated by liquid chromatography tandem mass spectrometry. Additionally, concentration cell-free DNA (cfDNA) in was measured, plasma C-reactive (CRP)...

Chlamydia trachomatis represents a group of human pathogenic obligate intracellular and gram-negative bacteria. The genome C. D comprises 894 open reading frames (ORFs). In this study the global expression genes in A, L2, which are responsible for different chlamydial diseases, was investigated using proteomics approach. Based on silver stained two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), gels with purified elementary bodies (EB) auto-radiography 35S-labeled proteins up to...