A5088140550- Gut microbiota and health
- Parasitic Infections and Diagnostics
- Clostridium difficile and Clostridium perfringens research
- Amoebic Infections and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Parasites and Host Interactions
- Enterobacteriaceae and Cronobacter Research
- Cancer-related molecular mechanisms research
- Microscopic Colitis
- Gastrointestinal motility and disorders
- Kruppel-like factors research
- Parasitic infections in humans and animals
- Pediatric health and respiratory diseases
- Viral Infections and Vectors
- Helminth infection and control
- Parasite Biology and Host Interactions
- Obesity and Health Practices
- Nutritional Studies and Diet
- Vector-borne infectious diseases
- Diet and metabolism studies
- IL-33, ST2, and ILC Pathways
- Bariatric Surgery and Outcomes
- Hidradenitis Suppurativa and Treatments
- Gestational Diabetes Research and Management
- Cervical Cancer and HPV Research
Statens Serum Institut
2016-2025
Institute of Parasitology
2021
Although the pathogenesis of hidradenitis suppurativa (HS) remains enigmatic, several factors point to potential involvement cutaneous microbiome. Insight into microbiome in HS using next-generation sequencing may provide novel data on microbiological diversity skin.To investigate follicular skin patients with and healthy controls.This case-control study obtained punch biopsy specimens from (lesional nonlesional) controls between October 1, 2014, August 2016. Data were analyzed March...
Blastocystis is a common single-celled intestinal parasitic genus, comprising several subtypes. Here, we screened data obtained by metagenomic analysis of faecal DNA for searching subtype-specific genes in coabundance gene groups, which are groups that covary across selection 316 human samples, hence representing originating from single subtype. The samples were 236 healthy individuals, 13 patients with Crohn's disease (CD) and 67 ulcerative colitis (UC). prevalence was 20.3% the individuals...
Blastocystis is a unicellular eukaryote found in the gastrointestinal tract of both human and other animal hosts. The clinical significance colonic colonization remains obscure. In this study, we used metabarcoding bioinformatics analyses to identify differences stool microbiota diversity between Blastocystis-positive Blastocystis-negative individuals (n = 1285). Alpha was significantly higher carriers. At phylum level, Firmicutes Bacteroidetes were enriched carriers, while Proteobacteria...
Blastocystis is a common intestinal parasite of unsettled clinical significance, which not easily detected by standard parasitological methods. The genus comprises at least 13 subtypes (STs) (which likely represent separate species), 9 have been found in humans. Recent data indicate that one the associated with disease. A quantitative TaqMan 5' nuclease real-time PCR (TaqMan PCR) including an internal process control (IPC) was developed for detection and shown to be applicable genomic DNAs...
Objective: A low prevalence of intestinal parasites has been identified in individuals with irritable bowel syndrome (IBS), but potential associations alterations the bacterial microbiome remain largely unexplored. We aimed to investigate relationship between and bacteria IBS order identify transkingdom microbial characteristics. Design: Stool samples were collected from Danish background population classified into (n = 119), unspecific gastrointestinal (GI) symptoms 114), asymptomatic...
There are challenges, when extracting bacterial DNA from specimens for molecular diagnostics, since fecal samples also contain human cells and many different substances derived food, cell residues medication that can inhibit downstream PCR. The purpose of the study was to evaluate two extraction methods in order choose most efficient method studying intestinal diversity using Denaturing Gradient Gel Electrophoresis (DGGE). In this study, a semi-automatic system (easyMag®, BioMérieux, Marcy...
Genetic polymorphisms in P. falciparum can be used to indicate the parasite's susceptibility antimalarial drugs as well its geographical origin. Both of these factors are key monitoring development and spread drug resistance. In this study, we combine multiplex PCR, custom designed dual indexing Miseq sequencing for high throughput SNP-profiling 457 malaria infections from Guinea-Bissau, at cost 10 USD per sample. By amplifying 15 genetic fragments, cover 20 resistance-conferring SNPs...
The whipworms Trichuris trichiura and suis are two parasitic nematodes of humans pigs, respectively. Although in human non-human primates historically have been referred to as T. trichiura, recent reports suggest that several spp. found primates.We sequenced annotated complete mitochondrial genomes recovered from a Uganda, an olive baboon the US, hamadryas Denmark, pigs Denmark Uganda. Comparative analyses using other published porcine host China françois' leaf-monkey (China) were performed,...
Application of next-generation sequencing (NGS) to genomic DNA extracted from sewage offers a unique and cost-effective opportunity study the genetic diversity intestinal parasites. In this study, we used amplicon-based NGS reveal differentiate several common luminal parasitic protists, specifically Entamoeba, Endolimax, Iodamoeba, Blastocystis, in samples Swedish treatment plants.Influent were subject gradient centrifugation, extraction PCR-based amplification using three primer pairs...
Diet composition may play a crucial role in shaping host immune responses and commensal gut microbiota populations. Functional foods,Bioactive dietary components, such as inulin, have been extensively studied for their bioactive properties, particularly modulating function reducing inflammation. Recent reports suggestedIt has shown that colonization with gastrointestinal parasitic worms (helminths) alleviate chronic inflammation through promotion of T-helper cell type (Th) 2 T-regulatory...
Maternal obesity is associated with adverse pregnancy outcomes. Probiotic supplementation during may have positive effects on blood glucose, gestational weight gain (GWG), and the risk of diabetes mellitus [GDM glycated hemoglobin (HbA1c)]. This feasibility study involved a daily probiotic intervention in obese pregnant women from early second trimester until delivery. The primary aim was to investigate effect GWG maternal glucose homeostasis (GDM HbA1c). Secondary aims were infant birth...
The human vagina harbor a rich microbiota. optimal state is dominated by lactobacilli that help to maintain health and prevent various diseases. However, the microbiota may rapidly change polymicrobial has been linked number of In present study, temporal changes vaginal in patients treated for sexually transmitted diseases or bacterial vaginosis (BV) untreated controls were studied 26 days. included 52 women with azithromycin, tetracyclines moxifloxacin suspected infection Chlamydia...
Comprehensive detection and differentiation of intestinal protists mostly rely on DNA-based methods. Here, we evaluated next-generation sequencing eukaryotic nuclear ribosomal genes (metabarcoding) for the in stool healthy Tunisian individuals. Thirty-six faecal DNA samples previously by microscopy ameboid species-specific PCRs were tested. The hypervariable regions V3-V4 V3-V5 18S rRNA gene amplified using three universal primer sets sequenced Illumina®MiSeq sequencing. In addition,...
Probiotics have been described to influence host health and prevent the risk of obesity by gut microbiome (GM) modulation. In a randomized double-blinded placebo-controlled feasibility study, we investigated whether Vivomixx® multi-strain probiotics administered 50 women with during pregnancy altered GM composition perinatal outcomes their infants up 9 months after birth. The mothers were followed four visits birth: at 3 d, 3, 6, delivery. monitored anthropometric measurements, fecal sample...
Asthma is a common chronic respiratory disease that affects children and adults can have serious impact on their quality of life. Factors contributing to the development asthma related exacerbations are multifactorial, with microbial communities colonizing airways possibly playing key role. The study included asthmatic (79) healthy (57) aged 5-16 years. Nasal throat swabs were collected, bacterial (16s rRNA) fungal (18s amplicon sequence analysis was performed. Diversity indices most...
Newly recognized endemic foci for human babesiosis include Europe, where Ixodes ricinus, a vector several species of Babesia, is the most commonly identified tick. Vector-based surveillance provides an early warning system emergence babesiosis, which likely to be under-reported at emerging sites. In present study, we set out screen I. ricinus collected from Danish domestic dogs in order identify whether humans Denmark are exposed parasite. A total 661 ticks (Ixodes spp.) were 345 during...
Limited evidence suggests that the consumption of polyphenols may improve glycaemic control and insulin sensitivity. The gut microbiome produces phenolic metabolites increases their bioavailability. A handful studies have suggested polyphenol alters composition. There are no data available investigating such effects in polyphenol-rich Montmorency cherry (MC) supplementation. total 28 participants (aged 40–60 years) were randomized to receive daily MC or glucose energy-matched placebo...
Acanthamoeba is a free-living amoeba of extensive genetic diversity. It may cause infectious keratitis (IK), which can also be caused by bacteria, fungi, and viruses.
Several parasite species are shared between humans and pigs. We explored the application of next-generation sequencing-based metabarcoding supplemented with real-time PCR to fecal DNAs from 259 samples 116 pigs in Denmark detect differentiate single-celled intestinal parasites zoonotic relevance. Enterocytozoon bieneusi, Balantioides coli, Giardia duodenalis were observed 34/37 (92%), 148/259 (57%), 86/259 (33%) samples, respectively. Entamoeba polecki ST1, E. ST3, hartmanni detected 104/259...