Toxoplasmosis has been recognized as parasitic zoonosis with the highest human incidence. The infection by parasite can lead to severe clinical manifestations in congenital toxoplasmosis and immunocompromised patients. Contamination occurs mainly foodborne ways especially consumption of raw or undercooked meat. In contrast other infections, is a chronic which would make its economic social impact much higher than even previously anticipated. Ovine meat was advanced major risk factor, so we...

Abstract Background The present study was conducted to investigate the serological survey of Toxoplasma antibodies in local.horses from three major regions: a neighbourhood city North (Sidi Thabet), on coast (Monastir) and middle (Battan) Tunisia (North Africa). Methods A total 158 serum samples were obtained clinically healthy horses which consisted 111 (32 female, 79 male) 2-10 years old 47 (11 36 older than 10 years. All tested for T. gondii using Modified Agglutination Test (MAT)....

In order to identify the phlebotomine sandfly populations in Tunisian leishmaniosis foci, an entomological survey was carried out through three seasons (2002-2003-2004) 19 visceral and cutaneous areas, located six bioclimatic zones. Sandfly collections were based on light sticky traps placed around human cases. 8,722 sandflies belonging 12 species collected. The dominance of subgenus Larroussius northern Phlebotomus papatasi south-western foci their co-dominance centre country is accordance...

Here, we determined the Toxoplasma gondii genotype in amniotic fluid, placenta, and cerebrospinal fluid samples from 14 congenital toxoplasmosis cases Tunisia, North Africa. Direct genotypic characterization of T. strains was performed by polymerase chain reaction (PCR) amplification six genetic markers (3' SAG2 , 5' SAG3 BTUB GRA6 APICO ) thereafter, analyzed restriction fragment-length polymorphism (RFLP). Samples were sequenced to resolve strain type whenever there unclear enzyme...

During September 2010, 133 female sand flies were caught inside houses of patients with cutaneous leishmaniasis in the focus for this disease southeastern Tunisia and subsequently dissected. One specimen was positive Leishmania protozoa. This fly species identified as Phlebotomus sergenti , parasite L. tropica. is first report P. involvement transmission tropica Tunisia.

North African gundis ( Ctenodactylus gundi ) were trapped in the Leishmania L. tropica focus of cutaneous leishmaniasis, situated southeast Tunisia and evaluated for infection by real-time kinetoplast DNA polymerase chain reaction (PCR). Species identification was performed internal transcribed spacer one (ITS1)-PCR-restriction fragment length polymorphism (RFLP) high-resolution melting (HRM) analysis 7SL RNA gene. Real-time PCR on blood positive 6 13 (46.2%) tested gundis. identified five...

Comprehensive detection and differentiation of intestinal protists mostly rely on DNA-based methods. Here, we evaluated next-generation sequencing eukaryotic nuclear ribosomal genes (metabarcoding) for the in stool healthy Tunisian individuals. Thirty-six faecal DNA samples previously by microscopy ameboid species-specific PCRs were tested. The hypervariable regions V3-V4 V3-V5 18S rRNA gene amplified using three universal primer sets sequenced Illumina®MiSeq sequencing. In addition,...

At birth, the biological diagnosis of congenital toxoplasmosis (CT) is essentially serological. To report results techniques used in neonatal CT. This was a descriptive study including newborns (NB) suspected having CT who were referred to Parasitology-Mycology laboratory Institut Pasteur Tunis over 17-year period. Newborns whose mothers had toxoplasmic infection during pregnancy, with either negative or unperformed antenatal diagnosis, included. The search for IgG and IgM antibodies...

Stool samples from 86 immunocompromised patients (51 human immunodeficiency virus (HIV)–infected and 35 with haematologic malignancies) were systematically screened for intestinal microspordiosis by microscopic examination polymerase chain reaction (PCR) using universal primer V1/PMP2. Nine (10.5%) showed amplification the predictive size of fragment (6 HIV–infected 3 myeloma). Only 5 out them (all patients) revealed positive microscopy. By means amplicons size, species–specific primers...

ABSTRACT Current methods for diagnosis of visceral leishmaniasis (VL) require invasive sampling procedures such as aspiration and/or blood drawing. The use diagnostic tests using oral fluid, which is easier to collect, would be more simple and practical VL diagnosis, especially under field conditions. Oral fluids from 37 patients 40 healthy controls were collected Oracol devices. Blood samples fluid specimens both groups analyzed by recombinant protein K39 (rK39) enzyme-linked immunosorbent...

Visceral leishmaniasis (VL) cases in children less than five years of age were recorded from 1996 through 2006 Tunisian pediatric departments. Mean incidence rates calculated for each the 215 districts study area. Averages annual rainfall and extreme values low temperatures winter high summer used to characterize climate district according its continentality index bioclimatic zone. A geographic information system a local indicator spatial association summarize properties VL distribution....

Cutaneous leishmaniasis (CL), a public health problem in Tunisia, is associated to three species: Leishmania (L.) infantum, L. major and killicki. Accurate sensitive procedures for the diagnostic of infection species identification are required enable adequate treatment appropriate control measures. Several PCR-methods applied diagnosis parasites such as PCR-restriction fragment length polymorphism (PCR-RFLP), DNA sequencing, hybridization probes real-time PCR (RT-PCR). In this study,...

Serologic detection of Toxoplasma gondii IgG antibodies is widely accepted as a means to determine immune status and susceptibility infection during pregnancy. However, current commercial kits present some drawbacks, such requirement for whole-parasite antigen preparation or interassay variability. To address these problems, the purpose this study was produce whole sequence recombinant T. SAG1 (rSAG1) assess its diagnostic performance in screening explore saliva-based method noninvasive...

La prévalence de la toxoplasmose a été étudiée dans le nord Tunisie chez 857 individus d'origine rurale et 564 urbaine. Les anticorps anti-toxoplasmiques ont recherchés par immunofluorescence indirecte (IFI) ELISA. population générale était 58,4 %. Elle significativement varié avec l'âge, passant 24,5 % à dix ans 52, I 20 ans, pour atteindre un maximum autour 70 vers l'âge 30 ans. A partir cet âge, les séroconversions semblent rares en juger faible proportion des sérums titres élevés IFI...

The authors report the identification of Leishmania strains isolated from Centre and South Tunisia. 266 were between 1998 2006 human (n = 221 strains) dogs 45 hosts. isoenzymatic exhibits presence in total five zymodemes belonging to three complexes: infantum, L. major killicki. All canine visceral leishmaniasis belonged infantum. zymodeme MON-1 was only one leishmaniasis. However, it is predominant beside MON-24 which detected two provinces (Monastir Kairouan) MON-80 for first time Kairouan...

The zoonotic potential of Cryptosporidium parvum was studied in an extensive cattle farming region northern Tunisia. Seventy fecal samples from pre-weaning calves and 403 children were examined by microscopy after modified Ziehl-Neelsen (MZN) staining. Positive specimens identified at a species level using 18S rRNA nested polymerase chain reaction (PCR) followed Restriction Fragment Length Polymorphism (RFLP) analysis. C. isolates subgenotyped sequence analysis the glycoprotein 60 (gp60)...

Antimonials remain the first-line treatment for various manifestations of leishmaniasis in most areas where disease is endemic, and increasing cases therapeutic failure associated with parasite resistance have been reported. In this study, we assessed molecular status 47 clinical isolates Leishmania causing visceral cutaneous from Algeria, Tunisia, southern France. total, examined 14 genes that shown to exhibit significant variations DNA amplification, mRNA levels, or protein expression...

Twenty cutaneous leishmaniasis (CL) cases were notified from December 2001 to February 2002, in a small village the district of Oueslatia (governorate Kairouan, central Tunisia) which is an endemic focus infantile visceral due Leishmania (L.) infantum and that had never been concerned previously by CL. The parasite typing two isolates obtained children have left region has identified L. killicki. This species only reported limited Tunisian Southeast. In October epidemiological survey with...