A5035038039- Cancer Cells and Metastasis
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Hippo pathway signaling and YAP/TAZ
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Digestive system and related health
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Cellular Mechanics and Interactions
- Microscopic Colitis
- Phagocytosis and Immune Regulation
- Inflammatory Bowel Disease
- IL-33, ST2, and ILC Pathways
- Developmental Biology and Gene Regulation
- T-cell and B-cell Immunology
- Congenital Diaphragmatic Hernia Studies
- Ferroptosis and cancer prognosis
- Renal and related cancers
- Diabetes Management and Research
- Psoriasis: Treatment and Pathogenesis
- Cancer Research and Treatments
University of Copenhagen
2012-2025
Novo Nordisk Foundation
2021-2025
Society for Immunotherapy of Cancer
2014
Generation of functionally mature organs requires exquisite control transcriptional programs governing cell state transitions during development. Despite advances in understanding the behavior adult intestinal stem cells and their progeny, regulators that emergence phenotype remain largely unknown. Using mouse fetal small organoids, we uncover differences between identify rare adult-like present organoids. This suggests organoids have an inherent potential to mature, which is locked by a...
T helper (TH) 17 cells are believed to play a pivotal role in development of inflammatory bowel disease, and their contribution intestinal inflammation has been studied various models colitis. TH17 produce range cytokines, some which potential targets for immunotherapy. However, blockade IL-17A alone with secukinumab was not effective Crohn's disease. In this regard, the pathogenic impact versus IL-17F during is still unresolved.Development IFN-γ-producing, IL-17A-producing, IL-17F-producing...
During intestinal organogenesis, equipotent epithelial progenitors mature into phenotypically distinct stem cells that are responsible for lifelong maintenance of the tissue. While morphological changes associated with transition well characterized, molecular mechanisms underpinning maturation process not fully understood. Here, we leverage organoid cultures to profile transcriptional, chromatin accessibility, DNA methylation, and three-dimensional (3D) conformation landscapes in fetal adult...
Organogenesis relies on the spatiotemporal balancing of differentiation and proliferation driven by an expanding pool progenitor cells. In mouse pancreas, lineage tracing at population level has shown that pancreas progenitors can initially give rise to all endocrine, ductal, acinar cells but become bipotent embryonic day 13.5, giving endocrine ductal However, dynamics individual self-renewal lineage-specific never been described. Using three-dimensional live imaging in vivo clonal analysis,...
Abstract Uncontrolled regeneration leads to neoplastic transformation 1–3 . The intestinal epithelium requires precise regulation during continuous homeostatic and damage-induced tissue renewal prevent transformation, suggesting that pathways unlinking tumour growth from regenerative processes must exist. Here, by mining RNA-sequencing datasets two damage models 4,5 using pharmacological, transcriptomics genetic tools, we identified liver X receptor (LXR) pathway activation as a adaptation...
The mammalian pancreas is a branched organ that does not exhibit stereotypic branching patterns, similarly to most other glands. Inside branches, it contains network of ducts undergo transition from unconnected microlumen mesh interconnected and finally treelike structure. This ductal remodeling poorly understood, both on microscopic macroscopic level. In this article, we quantify the properties at different developmental stages. We find pancreatic exhibits traits each stage change with time...
Spatiotemporal balancing of cellular proliferation and differentiation is crucial for postnatal tissue homoeostasis organogenesis. During embryonic development, pancreatic progenitors simultaneously proliferate differentiate into the endocrine, ductal acinar lineages. Using in vivo clonal analysis founder population pancreas here we reveal highly heterogeneous contribution single to organ formation. While some are bona fide multipotent contribute progeny all major cell lineages, also...
B cells positively contribute to immunity by antigen presentation CD4(+) T cells, cytokine production, and differentiation into antibody secreting plasma cells. Accumulating evidence implies that also possess immunoregulatory functions closely linked their capability of IL-10 secretion.Colitis development was followed in CD25(-) cell transplanted SCID mice co-transferred with exposed an enterobacterial extract (ebx-B cells). expression measured flow cytometry enzyme-linked immunosorbent...
Abstract During intestinal organogenesis, equipotent epithelial progenitors mature into phenotypically distinct stem cells that are responsible for life-long maintenance of the tissue. While morphological changes associated with transition well-characterized, molecular mechanisms underpinning maturation process not fully understood. Here, we leverage organoid cultures to profile transcriptional, chromatin accessibility, DNA methylation and 3D conformation landscapes defining fetal adult...
Animal tissue development relies on precise generation and deployment of specific cell types into sub-structures. Understanding how this process is regulated remains a major challenge biology. In many tissues, progresses through sequence dividing progenitor cells, each with decreasing potency, that balance their growth differentiation. Dividing cells thus face decision whether offspring shall differentiate or self-renew. This results in three possible modes division (symmetric self-renewing,...
Abstract The induction of active immunity against tumour‐associated antigens to prevent relapse cancer is a promising approach but has so far shown only low efficacy. This efficacy may in part be due clonal escape tumour cell variants by the downregulation antigen expression or inflammation‐induced dedifferentiation. Identification novel that at same time are essential for continued survival thus critical development vaccinations. At time, identification endogenous murine will help improve...
Meeting abstracts Adoptive cell transfer (ACT) using in vitro expanded T cells from biopsy material represents a highly promising treatment of disseminated cancer. ACT its present form is rather crude and improvements seem within reach. Recruitment transferred lymphocytes to the tumor site
Vaccination with tumor-antigen pulsed, monocyte-derived dendritic cells (DCs) has emerged as a promising strategy in cancer immunotherapy. The standard DC maturation cocktail consists of combination tumor necrosis factor-α (TNF-α)/interleukin (IL)-1β/IL-6 and prostaglandin E2 (PGE2) for generation DCs (sDCs). In order to improve IL-12p70 production cytotoxic T-lymphocyte (CTL) induction, novel composed TNF-α/IL-1β/ interferon (IFN)-α/IFN-γ polyinosinic:polycytidylic acid (Poly-I:C) been...