A5041617460- Oral microbiology and periodontitis research
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Bone Metabolism and Diseases
- Immune Cell Function and Interaction
- Antimicrobial Peptides and Activities
- NF-κB Signaling Pathways
- Proteoglycans and glycosaminoglycans research
- Oral Health Pathology and Treatment
- T-cell and B-cell Immunology
- Osteoarthritis Treatment and Mechanisms
- Sinusitis and nasal conditions
- Microbial infections and disease research
- Antifungal resistance and susceptibility
- Cell Adhesion Molecules Research
- IL-33, ST2, and ILC Pathways
- Gut microbiota and health
- HIV/AIDS oral health manifestations
- Temporomandibular Joint Disorders
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Alzheimer's disease research and treatments
- Cancer Cells and Metastasis
- Helicobacter pylori-related gastroenterology studies
- Foreign Language Teaching Methods
Karolinska University Hospital
2021-2024
Karolinska Institutet
2020-2024
University of Chile
2013-2021
University of Michigan–Ann Arbor
2018
University of Connecticut
2018
The Ohio State University
2018
University of Maryland, College Park
2018
University of California, San Francisco
2018
University at Buffalo, State University of New York
2018
Public and Science
2018
Abstract The intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape spatially organized in steady state healing murine colon. At conditions, demonstrate previously unappreciated molecular regionalization colon, which dramatically changes during mucosal healing. identified spatially-organized transcriptional programs healing, regions with dominant...
Therapeutic promotion of intestinal regeneration holds great promise, but defining the cellular mechanisms that influence tissue remains an unmet challenge. To gain insight into process mucosal healing, we longitudinally examined immune cell composition during damage and regeneration. B cells were dominant type in healing colon, single-cell RNA sequencing (scRNA-seq) revealed expansion IFN-induced subset experimental predominantly located damaged areas associated with colitis severity....
Periodontal disease is a of tooth-supporting tissues. It chronic with inflammatory nature and infectious etiology produced by dysbiotic subgingival microbiota that colonizes the gingivodental sulcus. Among several periodontal bacteria, Porphyromonas gingivalis (P. gingivalis) highlights as keystone pathogen. Previous reports have implied response measurable bone resorption are observed in young mice, even after short period infection P. gingivalis, which has been considered suitable model...
Abstract The alveolar bone resorption is a distinctive feature of periodontitis progression and determinant for tooth loss. Regulatory T lymphocytes (Tregs) display immuno-suppressive mechanisms tissue repairing functions, which are critical to support periodontal health. Tregs may become unstable dysfunctional under inflammatory conditions, can even accelerate destruction. In this study, experimental was associated with the progressive increased presence Th17 Treg-related mediators in...
It is well accepted that the presence of cytokines belonging to Th1/Th17/Th22 axis immuno-inflammatory response in joint environment, such as IL-1β, IL-17 and IL-22, respectively, are associated with pathogenesis several synovial degenerative disorders. During temporomandibular osteoarthritis (TMJ-OA), IL-1β have been implicated inflammation resorption sub-chondral bone; however, role Th22 TMJ-OA pathophysiology has not established. This study aimed compare expression Th1/Th17/Th22-type...
Introduction: Previous reports have proposed that Periodontal disease (PDis) predisposes to Alzheimer's (AD), both highly prevalent pathologies among the elderly. The bacteria Aggregatibacter actinomycetemcomitans (Aa), associated with most aggressive forms of PDis, are classified in different serotypes distinct virulence according antigenicity their lipopolysaccharide (LPS). Methods: Here, we determined effects purified LPS, from a, b or c Aa, on primary cultures microglia mixed hippocampal...
Abstract Uncontrolled regeneration leads to neoplastic transformation 1–3 . The intestinal epithelium requires precise regulation during continuous homeostatic and damage-induced tissue renewal prevent transformation, suggesting that pathways unlinking tumour growth from regenerative processes must exist. Here, by mining RNA-sequencing datasets two damage models 4,5 using pharmacological, transcriptomics genetic tools, we identified liver X receptor (LXR) pathway activation as a adaptation...
Background: Different serotypes of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis have been shown to induce differential dendritic cell (DC) responses. This study investigates whether cytokine CC‐chemokine receptor (CCR) production by DCs stimulated with different A. or P. is Toll‐like 2 (TLR2) and/or TLR4 dependent. Methods: were obtained from healthy individuals primed at a multiplicity infection (MOI) 10 in the presence absence anti‐TLR2 anti‐TLR4 blocking antibodies....
Two new T-helper (Th) phenotypes have been recently described and named Th9 Th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. This study was aimed to assess whether lymphocytes, through interleukin (IL)-9 IL-22 production, respectively, are associated with severity bone resorption.Gingival crevicular fluid samples biopsies were obtained from patients moderate-to-advanced chronic gingivitis, healthy controls. The levels for Th22-associated cytokines...
Aggregatibacter actinomycetemcomitans expresses several virulence factors that may contribute to the pathogenesis of periodontitis. Based on antigenicity O-polysaccharide component lipopolysaccharide (LPS), different A. serotypes have been described. Among them, serotype b has demonstrated a stronger capacity trigger Th1 and Th17-associated cytokine, CC-chemokine, CC-chemokine receptor production immune cells in vitro. With murine model experimental periodontitis, this investigation aimed...
Over the past few years, importance of interleukin-22 (IL-22) and T-helper (Th)22 lymphocytes in pathogenesis periodontitis has become apparent; however, there are still aspects that not addressed yet. Cells expressing IL-22 aryl hydrocarbon receptor (AhR), transcription factor master switch gene implicated differentiation function Th22 lymphocytes, have been detected periodontal tissues periodontitis-affected patients. In addition, associated with osteoclast their bone resorptive activity...
Cytokines are immunomodulatory proteins that orchestrate cellular networks in health and disease. Among these, interleukin (IL)-10 is critical for the establishment of intestinal homeostasis, as mutations components IL-10 signaling pathway result spontaneous colitis. Whether plays other than roles intestines poorly understood. Here, we report il10, il10ra, il10rb expressed zebrafish developing intestine early 3 days post fertilization. CRISPR/Cas9-generated il10-deficient larvae showed an...
Background and Objective Based on lipopolysaccharide (LPS) antigenicity, different Aggregatibacter actinomycetemcomitans serotypes have been described. Serotype b strains demonstrated a stronger capacity to trigger cytokine production dendritic cells (DCs). As DCs regulate the development of T‐lymphocyte lineages, objective this investigation was study response T lymphocytes after being stimulated with autologous primed bacterial belonging most prevalent A. in humans: a–c. Material Methods...
Abstract Background Encapsulation of Porphyromonas gingivalis has been demonstrated as responsible several host immunological changes, which have associated with the pathogenesis periodontitis. Using a murine model periodontitis and two isogenic non‐capsulated mutants P. , this study aimed to analyze whether encapsulation induces more severe alveolar bone resorption, loss is T‐helper (Th)1 Th17‐pattern immune response. Methods Experimental periodontal infections were generated by oral...
Summary During periodontitis, alveolar bone resorption is associated with activation of T helper type 17 (Th17) lymphocytes and receptor activator nuclear factor‐κB ligand ( RANKL ) ‐induced osteoclasts. We previously reported that serotype b Aggregatibacter actinomycetemcomitans has a higher capacity to trigger Th17‐type differentiation function in activated its lipopolysaccharide more potent immunogen compared the other serotypes. This study aimed investigate whether A. induces...
The adverse environmental conditions found in the periodontium during periodontitis pathogenesis stimulate local autophagy responses, mainly due to a continuous inflammatory response against dysbiotic subgingival microbiome. junctional epithelium represents main site of initial interaction between host and biofilm. Here, we investigated role keratinocytes (JEKs) Aggregatibacter actinomycetemcomitans or its purified lipopolysaccharides (LPS). Immunofluorescence confocal analysis revealed an...
Aggregatibacter actinomycetemcomitans is a Gram-negative oral bacterium with high immunostimulatory and pathogenic potential involved in the onset progression of periodontitis, chronic disease characterized by aberrant immune responses followed tooth-supporting bone resorption, which eventually leads to tooth loss. While several studies have provided evidence related virulence factors A. host cell death evasion, such as its most studied primate-specific factor, leukotoxin, role specific...
This study aimed to determine the expression of distinct matrix metalloproteinases, cytokines, and bone resorptive factors in temporomandibular joint osteoarthritis (TMJ-OA) patients their association with pain, mouth opening, subchondral degeneration.Twelve affected TMJ-OA (n = 5), disk displacement without reduction (DDWoR) 3), or (DDWR) 4) were selected. Joint pain was quantified by using visual analog scale, opening at maximum pain-free aperture, degeneration imaging. Synovial fluid...