A5055084815- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Oral microbiology and periodontitis research
- Nicotinic Acetylcholine Receptors Study
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical effects in animals
- Aluminum toxicity and tolerance in plants and animals
- Cholinesterase and Neurodegenerative Diseases
- Trace Elements in Health
- Amino Acid Enzymes and Metabolism
- Supramolecular Self-Assembly in Materials
- Dementia and Cognitive Impairment Research
- Dental Health and Care Utilization
- Optical Imaging and Spectroscopy Techniques
- Science, Technology, and Education in Latin America
- Memory and Neural Mechanisms
- Nerve injury and regeneration
- Ferroptosis and cancer prognosis
- Neurogenesis and neuroplasticity mechanisms
- Salivary Gland Disorders and Functions
- Aldose Reductase and Taurine
- Functional Brain Connectivity Studies
- Oral Health Pathology and Treatment
University of Chile
2016-2025
University of Valparaíso
2024
Instituto de Neurociencia Biomédica
2020-2022
University of Dental Medicine
2020
Research Network (United States)
2020
Universidade Federal do Rio de Janeiro
2001-2012
Alzheimer's disease (AD) and several other neurological disorders have been linked to the overactivation of glutamatergic transmission excitotoxicity as a common pathway neuronal injury. The beta-amyloid peptide (Abeta) is centrally related pathogenesis AD, previous reports demonstrated that blockade glutamate receptors prevents Abeta-induced death. We show taurine, beta-amino acid found at high concentrations in brain, protects chick retinal neurons culture against neurotoxicity Abeta...
Ryanodine receptors (RyR) amplify activity-dependent calcium influx via calcium-induced release. Calcium signals trigger postsynaptic pathways in hippocampal neurons that underlie synaptic plasticity, learning, and memory. Recent evidence supports a role of the RyR2 RyR3 isoforms these processes. Along with signals, brain-derived neurotrophic factor (BDNF) is key signaling molecule for plasticity spatial Upon binding to specific TrkB receptors, BDNF initiates complex modify structure...
Soluble amyloid β-peptide oligomers (AβOs), increasingly recognized as causative agents of Alzheimer's disease (AD), disrupt neuronal Ca2+ homeostasis and synaptic function. Here, we report that AβOs at sublethal concentrations generate prolonged signals in primary hippocampal neurons; incubation Ca2+-free solutions, inhibition ryanodine receptors (RyRs) or N-methyl-d-aspartate (NMDARs), preincubation with N-acetyl-l-cysteine abolished these signals. decreased (6 h) RyR2 RyR3 mRNA protein,...
Soluble oligomers of the amyloid-β peptide (AβOs) accumulate in Alzheimer's disease (AD) brain and have been implicated mechanisms pathogenesis. The neurotoxicity AβOs appears to be, at least part, due dysregulation glutamate signaling. Here, we show that promote extracellular accumulation d-serine, a co-agonist receptors N-methyl-d-aspartate subtype (NMDARs), hippocampal neuronal cultures. increase levels induced by was blocked sodium channel blocker tetrodotoxin (TTX), NMDAR...
Periodontal disease is a of tooth-supporting tissues. It chronic with inflammatory nature and infectious etiology produced by dysbiotic subgingival microbiota that colonizes the gingivodental sulcus. Among several periodontal bacteria, Porphyromonas gingivalis (P. gingivalis) highlights as keystone pathogen. Previous reports have implied response measurable bone resorption are observed in young mice, even after short period infection P. gingivalis, which has been considered suitable model...
Alzheimer's disease (AD) is the leading cause of dementia worldwide, but its pathophysiological phenomena are not fully elucidated. Many neurophysiological markers have been suggested to identify early cognitive impairments AD. However, diagnosis this remains a challenge for specialists. In present cross-sectional study, our objective was evaluate manifestations and mechanisms underlying visual-spatial deficits at stages AD.We combined behavioral, electroencephalography (EEG), eye movement...
Increased reactive oxygen species (ROS) generation and the ensuing oxidative stress contribute to Alzheimer’s disease pathology. We reported previously that amyloid- β peptide oligomers (A Os) produce aberrant Ca 2+ signals at sublethal concentrations decrease expression of type-2 ryanodine receptors (RyR2), which are crucial for hippocampal synaptic plasticity memory. Here, we investigated whether antioxidant agent astaxanthin (ATX) protects neurons from A Os-induced excessive mitochondrial...
We have previously reported that primary hippocampal neurons exposed to synaptotoxic amyloid beta oligomers (AβOs), which are likely causative agents of Alzheimer's disease (AD), exhibit abnormal Ca2+ signals, mitochondrial dysfunction and defective structural plasticity. Additionally, AβOs-exposed a decrease in the protein content type-2 ryanodine receptor (RyR2) channels, exert critical roles synaptic plasticity spatial memory processes. The antioxidant N-acetylcysteine (NAC) prevents...
Aims: Previous studies indicate that hippocampal synaptic plasticity and spatial memory processes entail calcium release from intracellular stores mediated by ryanodine receptor (RyR) channels. In particular, RyR-mediated Ca2+ is central for the dendritic spine remodeling induced brain-derived neurotrophic factor (BDNF), a neurotrophin stimulates complex signaling pathways leading to memory-associated protein synthesis structural plasticity. To examine if upregulation of type-2 (RyR2)...
Introduction: Previous reports have proposed that Periodontal disease (PDis) predisposes to Alzheimer's (AD), both highly prevalent pathologies among the elderly. The bacteria Aggregatibacter actinomycetemcomitans (Aa), associated with most aggressive forms of PDis, are classified in different serotypes distinct virulence according antigenicity their lipopolysaccharide (LPS). Methods: Here, we determined effects purified LPS, from a, b or c Aa, on primary cultures microglia mixed hippocampal...
This study aims to assess a new mobile application (app)’s efficacy in raising oral cancer awareness among older adults through educational videos and serious games. The app, named TEGO® (Tele-platform of Geriatric Dental Specialties), with video about prevention, oral-self-examination, gaming elements, like trivia word search puzzles reinforce the acquired knowledge was developed. Fifty-six patients, aged 60 80 years, were randomly selected from Clinic University Chile invited use app on...
Amyloid β peptide oligomers (AβOs), toxic aggregates with pivotal roles in Alzheimer´s disease, trigger persistent and low magnitude Ca2+ signals neurons. We reported previously that these signals, which arise from entry subsequent amplification by release through ryanodine receptor (RyR) channels, promote mitochondrial network fragmentation reduce RyR2 expression. Here, we examined if AβOs, inducing redox sensitive RyR-mediated release, stimulate Ca2+-uptake, ROS generation fragmentation,...
Significance The expression of genes involved in hippocampal synaptic plasticity, learning, and memory requires that Ca 2+ signals generated spines, dendrites, or the soma by neuronal stimulation reach nucleus. Here, we report neurons induces release mediated RyR2 channels, which contributes to nuclear signal generation. Suppression RyR-mediated inhibited activity-induced phosphorylation transcriptional regulator CREB Npas4 transcription factor RyR2, play crucial roles processes. We propose...
Ferroptosis, a newly described form of regulated cell death, is characterized by the iron-dependent accumulation lipid peroxides, glutathione depletion, mitochondrial alterations, and enhanced lipoxygenase activity. Inhibition peroxidase 4 (GPX4), key intracellular antioxidant regulator, promotes ferroptosis in different types. Scant information available on GPX4-induced hippocampal neurons. Moreover, role calcium (Ca2+) signaling remains elusive. Here, we report that RSL3, selective...