A5020686637- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Ion channel regulation and function
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Vitamin C and Antioxidants Research
- Sirtuins and Resveratrol in Medicine
- Antioxidant Activity and Oxidative Stress
- ATP Synthase and ATPases Research
- Cholesterol and Lipid Metabolism
- Retinal Development and Disorders
- Nail Diseases and Treatments
- Eosinophilic Disorders and Syndromes
- Medicinal Plants and Neuroprotection
- Dermatological and COVID-19 studies
- Signaling Pathways in Disease
- Anesthesia and Neurotoxicity Research
- Ferroptosis and cancer prognosis
- Oral microbiology and periodontitis research
- Parvovirus B19 Infection Studies
- Nerve injury and regeneration
- Plant Stress Responses and Tolerance
- Genital Health and Disease
- Biochemical effects in animals
University of Chile
2016-2025
Hospital de Clínicas
2025
Hospital Clínico de la Universidad de Chile
2023
Instituto de Neurociencia Biomédica
2021-2022
Clínica Las Condes
2016-2020
The role of the mitochondrial calcium uniporter (MCU) in energy dysfunction and hypertrophy heart failure (HF) remains unknown. In angiotensin II (ANGII)–induced hypertrophic cardiac cells we have shown that overexpress MCU present bioenergetic dysfunction. However, by silencing MCU, cell are prevented blocking overload, increase reactive oxygen species, activation nuclear factor kappa B–dependent proinflammatory signaling. Moreover, identified a calcium/calmodulin–independent protein kinase...
Increased reactive oxygen species (ROS) generation and the ensuing oxidative stress contribute to Alzheimer’s disease pathology. We reported previously that amyloid- β peptide oligomers (A Os) produce aberrant Ca 2+ signals at sublethal concentrations decrease expression of type-2 ryanodine receptors (RyR2), which are crucial for hippocampal synaptic plasticity memory. Here, we investigated whether antioxidant agent astaxanthin (ATX) protects neurons from A Os-induced excessive mitochondrial...
Aims: Previous studies indicate that hippocampal synaptic plasticity and spatial memory processes entail calcium release from intracellular stores mediated by ryanodine receptor (RyR) channels. In particular, RyR-mediated Ca2+ is central for the dendritic spine remodeling induced brain-derived neurotrophic factor (BDNF), a neurotrophin stimulates complex signaling pathways leading to memory-associated protein synthesis structural plasticity. To examine if upregulation of type-2 (RyR2)...
Introduction: Previous reports have proposed that Periodontal disease (PDis) predisposes to Alzheimer's (AD), both highly prevalent pathologies among the elderly. The bacteria Aggregatibacter actinomycetemcomitans (Aa), associated with most aggressive forms of PDis, are classified in different serotypes distinct virulence according antigenicity their lipopolysaccharide (LPS). Methods: Here, we determined effects purified LPS, from a, b or c Aa, on primary cultures microglia mixed hippocampal...
Abstract Postoperative delirium (POD), an acute cognitive dysfunction linked to morbidity and mortality, is characterized by memory impairments disturbances in consciousness, particularly patients aged 65 older. Neuroinflammation NAD+ imbalance are key mechanisms behind POD, leading synaptic deterioration. However, how surgery contributes POD neuroinflammation remains unclear, effective treatments lacking. Here we used a rodent model of bone fracture examine the impact on plasticity,...
Amyloid β peptide oligomers (AβOs), toxic aggregates with pivotal roles in Alzheimer´s disease, trigger persistent and low magnitude Ca2+ signals neurons. We reported previously that these signals, which arise from entry subsequent amplification by release through ryanodine receptor (RyR) channels, promote mitochondrial network fragmentation reduce RyR2 expression. Here, we examined if AβOs, inducing redox sensitive RyR-mediated release, stimulate Ca2+-uptake, ROS generation fragmentation,...
Significance The expression of genes involved in hippocampal synaptic plasticity, learning, and memory requires that Ca 2+ signals generated spines, dendrites, or the soma by neuronal stimulation reach nucleus. Here, we report neurons induces release mediated RyR2 channels, which contributes to nuclear signal generation. Suppression RyR-mediated inhibited activity-induced phosphorylation transcriptional regulator CREB Npas4 transcription factor RyR2, play crucial roles processes. We propose...
Ferroptosis, a newly described form of regulated cell death, is characterized by the iron-dependent accumulation lipid peroxides, glutathione depletion, mitochondrial alterations, and enhanced lipoxygenase activity. Inhibition peroxidase 4 (GPX4), key intracellular antioxidant regulator, promotes ferroptosis in different types. Scant information available on GPX4-induced hippocampal neurons. Moreover, role calcium (Ca2+) signaling remains elusive. Here, we report that RSL3, selective...
Introduction: Neuronal Ca2+ signals generated through the activation of Ca2+-induced release in response to activity-generated influx play a significant role hippocampal synaptic plasticity, spatial learning, and memory. We others have previously reported that diverse stimulation protocols, or different memory-inducing procedures, enhance expression endoplasmic reticulum-resident channels rat primary neuronal cells tissue. Methods Results: Here, we report induction long-term potentiation...
Astaxanthin (ASX) is a carotenoid pigment with strong antioxidant properties. We have reported previously that ASX protects neurons from the noxious effects of amyloid-β peptide oligomers, which promote excessive mitochondrial reactive oxygen species (mROS) production and induce sustained increase in cytoplasmic Ca2+ concentration. These properties make promising therapeutic agent against pathological conditions entail oxidative dysregulation. Here, we studied whether N-methyl-D-aspartate...
Cutaneous findings in patients with severe acute respiratory syndrome coronavirus 2 infection (SARS-CoV-2) are more common every day. Findings include, among others, pseudo-chilblains, vesicular, urticarial, and maculopapular eruptions. Livedo or necrosis is less and, most importantly, suggests occlusive vascular disease usually related to infection.1 Petechiae/purpuric rash can resemble dengue fever appear as a morbilliform rash. This spare palmoplantar skin mucosa presents confluent...
Hippocampal neuronal activity generates dendritic and somatic Ca2+ signals, which depending on stimulus intensity, rapidly propagate to the nucleus induce expression of transcription factors genes with crucial roles in cognitive functions. Soluble Amyloid-beta Oligomers (AβOs), main synaptotoxins engaged pathogenesis Alzheimer's disease, generate aberrant signals primary hippocampal neurons, increase their oxidative tone disrupt structural plasticity. Here, we explored effects...
Hippocampal neuronal activity generates dendritic and somatic Ca2+ signals, which, depending on stimulus intensity, rapidly propagate to the nucleus induce expression of transcription factors genes with crucial roles in cognitive functions. Soluble amyloid-beta oligomers (AβOs), main synaptotoxins engaged pathogenesis Alzheimer's disease, generate aberrant signals primary hippocampal neurons, increase their oxidative tone disrupt structural plasticity. Here, we explored effects sub-lethal...
*Department of Dermatology, Clínica Las Condes, Santiago, Chile; †University the Andes, and ‡Department Pathology, Chile. Reprints: Andrea Lobos, University Santiago 1240000, Chile (e-mail: [email protected]). The authors declare no conflicts interest.