A5053532767- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Hippo pathway signaling and YAP/TAZ
- Adhesion, Friction, and Surface Interactions
- Digestive system and related health
- Inflammatory Bowel Disease
- Knee injuries and reconstruction techniques
- Vitamin K Research Studies
- Antioxidant Activity and Oxidative Stress
- Gut microbiota and health
- Vitamin C and Antioxidants Research
- Microscopic Colitis
- Epigenetics and DNA Methylation
- Vitamin D Research Studies
- Total Knee Arthroplasty Outcomes
- Barrier Structure and Function Studies
- Renal and related cancers
- Genetics, Aging, and Longevity in Model Organisms
- Sports injuries and prevention
- Helicobacter pylori-related gastroenterology studies
- Mitochondrial Function and Pathology
- Meat and Animal Product Quality
- Circadian rhythm and melatonin
- Pesticide Residue Analysis and Safety
- Pharmacological Effects and Assays
Novo Nordisk Foundation
2023-2025
University of Copenhagen
1997-2025
Gentofte Hospital
1999
Generation of functionally mature organs requires exquisite control transcriptional programs governing cell state transitions during development. Despite advances in understanding the behavior adult intestinal stem cells and their progeny, regulators that emergence phenotype remain largely unknown. Using mouse fetal small organoids, we uncover differences between identify rare adult-like present organoids. This suggests organoids have an inherent potential to mature, which is locked by a...
During intestinal organogenesis, equipotent epithelial progenitors mature into phenotypically distinct stem cells that are responsible for lifelong maintenance of the tissue. While morphological changes associated with transition well characterized, molecular mechanisms underpinning maturation process not fully understood. Here, we leverage organoid cultures to profile transcriptional, chromatin accessibility, DNA methylation, and three-dimensional (3D) conformation landscapes in fetal adult...
The intestinal epithelium ensures uptake of vital nutrients and acts as a barrier between luminal contents the underlying immune system. In inflammatory bowel diseases, such ulcerative colitis (UC), this is compromised, patients experience debilitating symptoms. Here, we perform single-cell RNA profiling epithelial cells outline patterns cell fate decisions in healthy individuals UC patients. We demonstrate that hierarchical behavior are altered identify unique cellular states associated...
The intestinal epithelium has a remarkably high turnover in homeostasis. It remains unresolved how this is orchestrated at the cellular level and behavior of stem progenitor cells ensures tissue maintenance. To address this, we combined quantitative fate mapping three complementary mouse models with mathematical modeling single-cell RNA sequencing. Our integrated approach generated spatially temporally defined model crypt maintenance based on two cycling populations: crypt-bottom...
Epidemiological studies have shown that subnormal levels of vitamin D (25[OH]D) are associated with a more aggravated clinical course ulcerative colitis [UC]. Despite an increased focus on the therapeutic importance and receptor [VDR] signalling, mechanisms underlying effects D-VDR axis UC remain elusive. Therefore, we aimed to investigate whether exposure active (1,25[OH]2D3/VDR) signalling in human organoids could influence maintenance colonic epithelium.
Seventy patients met our inclusion criteria in this retrospective study, all with an arthroscopic/arthrotomic‐verified isolated total anterior cruciate ligament (ACL)‐rupture and a minimum follow‐up period of 3 years no associated lesions. Due to emigration/death, were not available for follow‐up. Of the remaining 67, 25 underwent secondary ACL‐reconstruction, equivalent failure rate initial non‐operative treatment 37%. All initially treated conservatively. This left 42 – 9 answered...
The intestinal epithelium has a remarkably high turnover in homeostasis. It remains unresolved how is orchestrated at the cellular level and behaviour of stem progenitor cells ensures tissue maintenance. To address this, we combined quantitative fate mapping three complementary mouse models with mathematical modelling single-cell RNA sequencing. Our integrated approach generated spatially temporally defined model crypt maintenance that based on two cycling populations: crypt-based columnar...
The intestinal epithelium is constantly exposed to microbes residing in the lumen. Traditionally, response microbial interactions has been studied cell lines derived from cancerous tissues, e.g. Caco-2. It is, however, unclear how responses these cancer reflect of a normal and whether there might be strain-specific effects. To address questions, we organoids small intestine cohort healthy individuals. Culturing on flat laminin matrix induced their differentiation, facilitating analysis via...
Abstract During intestinal organogenesis, equipotent epithelial progenitors mature into phenotypically distinct stem cells that are responsible for life-long maintenance of the tissue. While morphological changes associated with transition well-characterized, molecular mechanisms underpinning maturation process not fully understood. Here, we leverage organoid cultures to profile transcriptional, chromatin accessibility, DNA methylation and 3D conformation landscapes defining fetal adult...
Vitamin C (vitC) deficiency is associated with increased cardiovascular disease risk, but its specific interplay arteriolar function unclear. This study investigates the effect of vitC in guinea pigs on plasma biopterin status and vasomotor responses coronary arteries exposed to vasoconstrictor/-dilator agents. Dunkin Hartley female (n = 32) were randomized high (1500 mg/kg diet) or low (0 50 for 10-12 weeks. At euthanasia, artery segments dissected mounted a wire-myograph. Vasomotor...
Development of complex nervous systems requires precisely controlled neurogenesis. The generation and specification neurons occur through the transcriptional post-transcriptional control regulatory networks. In vertebrates invertebrates, proneural basic-helix-loop-helix (bHLH) family transcription factors has multiple functions in Here, we identified LIN-32/Atonal bHLH factor as a key regulator URXL/R oxygen-sensing neuron development Caenorhabditis elegans. When expression is lost, URX...
Summary The intestinal epithelium forms a barrier to the lumen and ensures uptake of vital nutrients. During inflammatory diseases, such as ulcerative colitis (UC), this is compromised. Here, we perform single-cell RNA sequencing (scRNA-seq) epithelial cells outline patterns cell fate decisions in healthy individuals patients with UC. We demonstrate that lineage biased progenitors are major sources normal tissue replenishment, behavior profoundly altered furthermore identify unique...
Introduction: An increased number of studies have recently elucidated both the importance vitamin D in pathogenesis ulcerative colitis (UC), but also role its receptor, receptor (VDR), development and progression experimental colitis. Despite an focus on VDR signaling, mechanisms how they affect UC remains elusive. Methods: We strived to reveal functional human colonic epithelium regarding effect epithelial homeostasis, for maintaining cellular composition intestinal barrier. Intestinal...