A5101896324- Coordination Chemistry and Organometallics
- Angiogenesis and VEGF in Cancer
- Organometallic Complex Synthesis and Catalysis
- Wnt/β-catenin signaling in development and cancer
- Axon Guidance and Neuronal Signaling
- Lymphatic System and Diseases
- Synthesis and characterization of novel inorganic/organometallic compounds
- Cancer-related gene regulation
- Synthetic Organic Chemistry Methods
- Cellular Mechanics and Interactions
- Inorganic and Organometallic Chemistry
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Asymmetric Synthesis and Catalysis
- Cellular transport and secretion
- RNA Research and Splicing
- Catalytic Cross-Coupling Reactions
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Asymmetric Hydrogenation and Catalysis
- Hippo pathway signaling and YAP/TAZ
- Organic and Molecular Conductors Research
- Caveolin-1 and cellular processes
- Cyclopropane Reaction Mechanisms
- Multi-Agent Systems and Negotiation
Friedrich Schiller University Jena
2014-2022
McGill University
2004-2012
Montreal Neurological Institute and Hospital
2004-2012
Canadian Institutes of Health Research
2012
University of Buenos Aires
2012
Consejo Nacional de Investigaciones Científicas y Técnicas
2012
Children's Hospital of Wisconsin
2012
Medical College of Wisconsin
2012
Genentech
2009-2010
PDL BioPharma (United States)
2009
Vascular sprouting is a key process-driving development of the vascular system. In this study, we show that neuropilin-2 (Nrp2), transmembrane receptor for lymphangiogenic endothelial growth factor C (VEGF-C), plays an important role in lymphatic vessel sprouting. Blocking VEGF-C binding to Nrp2 using antibodies specifically inhibits developing tip cells vivo. vitro analyses modulates cell extension and prevents stalling retraction during sprout formation. Genetic deletion reproduces defects...
VEGF-B, a homolog of VEGF discovered long time ago, has not been considered an important target in antiangiogenic therapy. Instead, it received little attention from the field. In this study, using different animal models and multiple types vascular cells, we revealed that although VEGF-B is dispensable for blood vessel growth, critical their survival. Importantly, survival effect only on endothelial but also pericytes, smooth muscle stem/progenitor cells. vivo, targeting inhibited both...
Neuropilin-1 (NRP1) was first described as a receptor for the axon guidance molecule, Semaphorin3A, regulating development of nervous system. It later shown that NRP1 is an isoform-specific vascular endothelial growth factor (VEGF), specifically VEGF165. Much interest has been placed on role various VEGF isoforms in biology. Here we report blocking function, using recently antibody inhibits VEGF165 binding to NRP1, surprisingly reduces VEGF121-induced migration and sprout formation cells....
No going back The mammalian spinal cord coordinates neuronal systems across the body. Axons that cross midline during development first need permission to and then instruction not keep crossing forth. Jaworski et al. studied axonal guidance receptor ROBO3 found a ligand NELL2 in mice appears help this process. Science , issue p. 961
Cadherins are single pass transmembrane glycoproteins which mediate calcium dependent cell−cell adhesion by homophilic interactions. To reveal the molecular details of binding and homoassociation, we recombinantly expressed in Escherichia coli a domain pair consisting first two domains E-cadherin (ECAD12) 1, 2, 5. ECAD12 encompasses most N-terminal four putative calcium-binding pockets extracellular region E-cadherin. Equilibrium dialysis experiments revealed that do not bind Ca2+, but was...
The expression of N-cadherin (NCAD) has been shown to correlate with increased tumor cell motility and metastasis. However, NCAD-mediated adhesion is a robust phenomenon therefore seems be inconsistent the "release" from intercellular required for invasion. We show that in most invasive melanoma brain cells, altered posttranslational processing results abundant nonadhesive precursor (proNCAD) at surface, although total NCAD levels remain constant. demonstrate aberrantly processed proNCAD...
ABSTRACT Here we present the cloning, expression and immunocytochemical localization of a novel 24 kDa protein, designated spinalin, which is in spines operculum Hydra nematocysts. Spinalin cDNA clones were identified by situ hybridization to differentiating nematocytes. Sequencing full-length clone revealed presence an N-terminal signal peptide, suggesting that mature protein sorted via endoplasmic reticulum post-Golgi vacuole nematocyst formed. The region spinalin (154 residues) very rich...
The direct synthesis of iodomethyltrimethylsilane with calcium and magnesium in ether yields the corresponding adducts [(thf)4Ca(I)(CH2SiMe3)] (1a) [(Et2O)2Mg(I)(CH2SiMe3)] (2a). 1,4-dioxane method allows shifting Schlenk equilibrium toward bis(trimethylsilylmethyl)magnesium 2b. After substitution ligated thf ligands by tetrahydropyran (thp) crystalline [(thp)4Ca(I)(CH2SiMe3)] (1b) can be isolated. Ca–C Ca–I bond lengths 252.7(3) 319.11(3) pm represent characteristic values. Steric repulsion...
Classical cadherins, which are adhesion molecules functioning at the CNS synapse, synthesized as adhesively inactive precursor proteins in endoplasmic reticulum (ER). Signal sequence and prodomain cleavage ER Golgi apparatus, respectively, activates their adhesive properties. Here, we provide first evidence for sorting of nonadhesive N-cadherin (ProN) to neuronal surface, where it coexists with competent mature (N-cad), generating a spectrum strengths. In cultured hippocampal neurons, high...
The direct synthesis of organocalcium compounds (heavy Grignard reagents) by the reduction organyl halides with activated calcium powder succeeded in a straightforward manner for organic bromides and iodides that are bound at sp2 -hybridized carbon atoms. Extension this strategy to alkyl was very limited, only trialkylsilylmethyl allowed isolation corresponding heavy reagents. Substitution one hydrogen atom methylene moiety phenyl or methyl group directed toward Wurtz-type coupling formation...
N-cadherin comprises five homologous extracellular domains, a transmembrane, and cytoplasmic domain. The domains of play important roles in homophilic cell adhesion, but the contribution each domain to this phenomenon has not been fully evaluated. In particular, following questions remain unanswered: what is minimal combination that can generate how organization related adhesive strength, does serve facilitate interaction? To address these issues, we made serial constructs produced various...
Neuropilin-1 (NRP1) acts as a co-receptor for class 3 semaphorins and vascular endothelial growth factor is an attractive angiogenesis target cancer therapy. In addition to the transmembrane form, naturally occurring soluble NRP1 proteins containing part of extracellular domain have been identified in tissues cell line. We developed ELISAs study existence circulating quantify it serum. As measured by ELISAs, levels mice, rats, monkeys humans were 427 ± 77, 20 3, 288 86 322 82 ng/ml (mean...