Eric F. Wawrousek

ORCID: 0000-0003-2959-911X
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About
Contact & Profiles
Research Areas
  • Connexins and lens biology
  • Heat shock proteins research
  • Immune Response and Inflammation
  • Immunotherapy and Immune Responses
  • T-cell and B-cell Immunology
  • Retinal Development and Disorders
  • RNA regulation and disease
  • Psoriasis: Treatment and Pathogenesis
  • Retinal Diseases and Treatments
  • Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • interferon and immune responses
  • Ocular Surface and Contact Lens
  • Ocular Diseases and Behçet’s Syndrome
  • Calpain Protease Function and Regulation
  • Glaucoma and retinal disorders
  • Intraocular Surgery and Lenses
  • Ubiquitin and proteasome pathways
  • Cell Adhesion Molecules Research
  • Biochemical effects in animals
  • Advanced Glycation End Products research
  • RNA and protein synthesis mechanisms
  • Molecular Biology Techniques and Applications
  • Yersinia bacterium, plague, ectoparasites research
  • Retinopathy of Prematurity Studies
  • Ocular Infections and Treatments

National Eye Institute
2008-2017

National Institutes of Health
2008-2017

Eunice Kennedy Shriver National Institute of Child Health and Human Development
2011

Institut thématique Génétique, génomique et bioinformatique
2009

Laboratory of Molecular Genetics
2002-2006

University of South Dakota
2005

Sioux Valley Hospital
2005

Guangzhou Medical University
2005

Laboratoire Souterrain à Bas Bruit
2002-2005

United States Department of Health and Human Services
2004

We noted an unexpected inheritance pattern of lesions in several strains gene-manipulated mice with ocular phenotypes. The lesions, which appeared at various stages backcross to C57BL/6, bore resemblance the rd8 retinal degeneration phenotype. set out examine prevalence this mutation induced mutant mouse lines, vendor C57BL/6 and widely used embryonic stem cells.Ocular were evaluated by fundus examination histopathology. Detection genetic level was performed PCR appropriate primers. Data...

10.1167/iovs.12-9662 article EN Investigative Ophthalmology & Visual Science 2012-03-24

The murine alpha B-crystallin gene was cloned and its expression examined. In the mouse, significant levels of RNA were detected not only in lens but also heart, skeletal muscle, kidney, lung; low trace brain spleen, respectively. species lung, brain, spleen 400 to 500 bases larger than that other tissues. Transcription lens, initiated at same position. A mouse mini-gene constructed introduced into germ line mice, demonstrated parallel endogenous gene. Transgene always while kidney lung...

10.1128/mcb.9.3.1083 article EN Molecular and Cellular Biology 1989-03-01

VEGF-B, a homolog of VEGF discovered long time ago, has not been considered an important target in antiangiogenic therapy. Instead, it received little attention from the field. In this study, using different animal models and multiple types vascular cells, we revealed that although VEGF-B is dispensable for blood vessel growth, critical their survival. Importantly, survival effect only on endothelial but also pericytes, smooth muscle stem/progenitor cells. vivo, targeting inhibited both...

10.1073/pnas.0813061106 article EN Proceedings of the National Academy of Sciences 2009-04-07

alpha A-crystallin (alpha A) and B-crystallin B) are among the predominant proteins of vertebrate eye lens. In vitro, alpha-crystallins, which isolated together as a high molecular mass aggregate, exhibit number properties, most interesting is their ability to function chaperones for other proteins. Here we begin examine in vivo functions alpha-crystallin by generating mice with targeted disruption A gene. Mice that homozygous disrupted allele produce no detectable lenses, based on protein...

10.1073/pnas.94.3.884 article EN Proceedings of the National Academy of Sciences 1997-02-04

The lens structural protein delta-crystallin and the metabolic enzyme argininosuccinate lyase (ASL; L-argininosuccinate arginine-lyase, EC 4.3.2.1) have striking sequence similarity. We demonstrated that duck has enormously high ASL activity, while chicken lower but significant activity. lenses of these birds had much greater activity than other tissues, suggesting is being expressed at unusually levels as a component. In Southern blots human genomic DNA, delta 1-crystallin cDNA hybridized...

10.1073/pnas.85.10.3479 article EN Proceedings of the National Academy of Sciences 1988-05-01

To create and evaluate a mouse model of human X-linked juvenile retinoschisis (XLRS) then investigate whether supplementing with the retinoschisin protein by gene delivery can reverse abnormal "electronegative" electroretinogram (ERG) retinal response.An (Rs1h-KO) was created substituting neomycin resistance cassette for exon 1 1.6 kb intron Rs1h, murine orthologue RS-1 gene. RS evaluated immunohistochemistry Western blot analysis polyclonal N-terminus antibody. Retinal function...

10.1167/iovs.04-0576 article EN Investigative Ophthalmology & Visual Science 2004-08-23

Abstract Th1 and Th17 cells are characterized by their expression of IFN-γ or IL-17, respectively. The finding Th producing both IL-17 suggested, however, that certain may modify selective cytokine expression. In this study, we examined changes in an experimental system which polarized specific against hen egg lysozyme induce ocular inflammation recipient mice expressing eyes. Whereas only was expressed eyes mice, substantial proportions donor possibility nonpolarized preparations were...

10.4049/jimmunol.181.10.7205 article EN The Journal of Immunology 2008-11-15

In phagocytic cells, including the retinal pigment epithelium (RPE), acidic compartments of endolysosomal system are regulators both phagocytosis and autophagy, thereby helping to maintain cellular homeostasis. The acidification is modulated by a proton pump, V-ATPase, but mechanisms that direct activity V-ATPase remain elusive. We found in RPE CRYBA1/βA3/A1-crystallin, lens protein also expressed RPE, localized lysosomes, where it regulates modulating controlling autophagy. demonstrated...

10.4161/auto.27292 article EN Autophagy 2014-01-23

αA- and αB-crystallins are molecular chaperones expressed at low levels in lens epithelial cells, their expression increases dramatically during differentiation to fibers. However, the functions of cells have not been studied detail. In this study, relative ability αB-crystallin, protecting from apoptotic cell death was determined. The introduction αA-crystallin transformed human (HLE) B-3 line (which expresses endogenous αB-crystallin) led a nearly complete protection induced by...

10.1074/jbc.m004233200 article EN cc-by Journal of Biological Chemistry 2000-11-01

The presence of increased ubiquitinated proteins and amyloid oligomers in failing human hearts strikingly resembles the characteristic pathology brain many neurodegenerative diseases. ubiquitin–proteasome system (UPS) is responsible for degradation most cellular plays essential roles virtually all processes. UPS impairment by aberrant protein aggregation was previously shown cell culture but remains to be demonstrated intact animals. Mechanisms underlying are poorly understood. We report...

10.1161/01.res.0000189262.92896.0b article EN Circulation Research 2005-10-07

We developed a genetic mouse model of open-angle glaucoma by expression mutated myocilin (Myoc) in transgenic (Tg) mice. The Tyr423His point mutation, corresponding to the severe glaucoma-causing Tyr437His mutation human MYOC gene, was introduced into bacterial artificial chromosome DNA encoding full-length Myoc gene and long flanking regions. Both wild-type (Wt) Tg animals expressed tissues irido-corneal angle sclera. Expression induced accumulation cell cytoplasm prevented its secretion...

10.1523/jneurosci.3020-06.2006 article EN cc-by-nc-sa Journal of Neuroscience 2006-11-15

Abstract Ocular inflammation leads to vision loss through the destruction and scarring of delicate tissues along visual axis. To identify inflammatory mediators involved in this process, we used real time RT-PCR quantify expression mRNA transcripts 34 cytokines, 26 chemokines, 14 chemokine receptors at certain points during T cell-mediated ocular inflammation. We induced disease by adoptive transfer Ag-specific Th1 or Th2 cells into recipients expressing target Ag their eyes. also compared...

10.4049/jimmunol.168.5.2483 article EN The Journal of Immunology 2002-03-01

RX, a homeodomain-containing protein essential for proper eye development (Mathers, P. H. Grinberg, A., Mahon, K. and Jamrich, M. (1997) <i>Nature</i> 387, 603–607), binds to the photoreceptor conserved element-1 (PCE-1/Ret 1) in cell-specific arrestin promoter stimulates gene expression. RX is found many retinal cell types including cells. Another protein, CRX, which OTX element stimulate activity, exclusively cells (Chen, S., Wang, Q. L., Nie, Z., Sun, H., Lennon, G., Copeland, N....

10.1074/jbc.275.2.1152 article EN cc-by Journal of Biological Chemistry 2000-01-01

Alexander disease (AxD) is a primary disorder of astrocytes caused by dominant mutations in the gene for glial fibrillary acidic protein (GFAP). These lead to aggregation and formation Rosenthal fibers, complex astrocytic inclusions that contain GFAP, vimentin, plectin, ubiquitin, Hsp27 αB-crystallin. The small heat shock αB-crystallin (Cryab) regulates GFAP assembly, elevation Cryab consistent feature AxD; however, its role fibers AxD pathology not known. Here, we show mouse models loss...

10.1093/hmg/ddp013 article EN Human Molecular Genetics 2009-01-07

Recently reported lines of Th9 cells, producing IL-9 and IL-10, were generated by polarization with IL-4 TGF-β activation Abs against CD3 CD28. In this paper, we analyzed features similarly polarized but activated the "natural mode" (i.e., exposure CD4 cells to their target Ag, hen egg lysozyme [HEL] APCs). Main observations are following: 1) both IL-10 expressed line strikingly different kinetics, being produced rapidly, reaching a peak on day 3 in culture declining sharply thereafter,...

10.4049/jimmunol.1001676 article EN The Journal of Immunology 2010-10-23

αA-Crystallin (αA) is a member of the small heat shock protein (sHSP) family and has ability to prevent denatured proteins from aggregating <i>in vitro</i>. Lens epithelial cells express relatively low levels αA, but in differentiated fiber cells, αA most abundant soluble protein. The lenses αA-knock-out mice develop opacities at an early age, implying critical role for maintenance cell transparency. However, function α-crystallin lens epithelium unknown. To investigate physiological we used...

10.1074/jbc.273.47.31252 article EN cc-by Journal of Biological Chemistry 1998-11-01

Overexpression studies have shown that the small heat shock proteins (sHSP) protect myocardium from ischemia-reperfusion (I/R)-induced damage. However, gene deletion are necessary to demonstrate whether sHSPs required for protection. The genes alphaB-crystallin (alphaBC) and HSPB2, two expressed in high levels heart, close proximity one another; as a result, both were disrupted recently generated knockout (KO) mouse line. alphaBC/HSPB2 KO line is currently only model features disruption of...

10.1152/ajpheart.00715.2003 article EN AJP Heart and Circulatory Physiology 2004-02-06

Abstract The chicken delta-crystallin locus consists of 2 nonallelic, tandemly arranged genes (5'-delta 1-delta 2-3'). Only the delta 1 gene is known to be expressed. nucleotide sequence for has been reported recently (Nickerson, J.M., Wawrousek, E.F., Hawkins, J.W., Wakil, A.S., Wistow, G.J., Thomas, G., Norman, B.L., and Piatigorsky, J. (1985) Biol. Chem. 260, 9100-9105; Ohno, M., Sakamoto, H., Yasuda, K., Okada, T.S., Shimura, Y. Nucleic Acids Res. 13, 1593-1606). We now report 4-kilobase...

10.1016/s0021-9258(17)36127-6 article EN cc-by Journal of Biological Chemistry 1986-01-01

During the early phase of experimental autoimmune uveitis (EAU), before macrophages infiltrate retina and uvea, photoreceptor mitochondrial oxidative stress, nitration proteins, release cytochrome c have been observed. However, no apoptosis has detected during this phase. In study, alphaA-crystallin upregulation in its antiapoptotic protective role were evaluated EAU.Gene microarrays first used to identify upregulated genes retinas with EAU. Among highly crystallins, alphaA was confirmed by...

10.1167/iovs.07-1259 article EN Investigative Ophthalmology & Visual Science 2008-03-01

delta-Crystallin is the principal structural protein of embryonic chicken lens. In chicken, there are two linked delta-crystallin genes arranged in same transcriptional polarity (5'-delta 1-delta 2-3'). Here we have sequenced delta 1 crystallin gene and almost 4.3 kilobases its 5' flanking region. The 7766 base pairs length has 17 exons. At least one lariat branch consensus sequence been identified each intron. Analysis deduced using a program which accounts for conservative amino acid...

10.1016/s0021-9258(17)39335-3 article EN cc-by Journal of Biological Chemistry 1985-08-01
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