A5086741128- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cellular Mechanics and Interactions
- Renal cell carcinoma treatment
- Peptidase Inhibition and Analysis
- Lymphoma Diagnosis and Treatment
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Viral-associated cancers and disorders
- Glioma Diagnosis and Treatment
- Pregnancy and preeclampsia studies
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- COVID-19 Clinical Research Studies
- Tissue Engineering and Regenerative Medicine
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Eosinophilic Disorders and Syndromes
- Cancer Cells and Metastasis
Institute of Molecular Biology and Genetics
2013-2024
Kyiv Academic University
2024
National Academy of Sciences of Ukraine
2016-2023
Instituto de Biomedicina y Genética Molecular de Valladolid
2009-2020
Institute of Urology of the National Academy of Medical Sciences of Ukraine
2019
Institute of Cell Biology
2016-2019
The critical point for successful treatment of cancer is diagnosis at early stages tumor development. Cancer cell-specific methylated DNA has been found in the blood patients, indicating that cell-free (cfDNA) circulating a convenient tumor-associated marker. Therefore cfDNA can be used as minimally invasive diagnostic We analysed concentration plasma and methylation six suppressor genes samples 27 patients with renal 15 healthy donors controls. concentrations from was measured using two...
This study aimed to identify the impact of mesenchymal stem cell transplantation on safety and clinical outcomes patients with severe COVID-19. research focused how lung functional status, miRNA, cytokine levels changed following in COVID-19 pneumonia their correlation fibrotic changes lung. involved 15 conventional anti-viral treatment (Control group) 13 after three consecutive doses combined MSC (MCS group). ELISA was used measure levels, real-time qPCR for miRNA expression, computed...
Background Scaffolding proteins of the intersectin (ITSN) family, ITSN1 and ITSN2, are crucial for initiation stage clathrin-mediated endocytosis. These closely related but have implications in distinct pathologies. To determine how these could be separated certain cell pathways we performed a comparative study ITSNs. Methodology/Principal Findings We shown that endogenous ITSN2 colocalize form complex cells. A structural comparison five SH3 domains, which mediated most ITSNs protein-protein...
Summary. Aim: To analyze the expression levels of hsa-miR-30c-5p and hsa-miR-138-1 in tumors patients with renal cell carcinoma to determine whether they could be used as diagnostic markers. Materials Methods: The relative was compared paired samples kidney tumor tissue conventionally normal adjacent tumor. Results: We found a significant decrease miR-30c-5p miR-138-1 tissues even cases early stage cancer. In addition, lower Fuhrman grade G3 + G4 G2. However, we no association between major...
Aim. Intersectin 1 (ITSN1) gene encodes an evolutionarily conserved adaptor protein that functions in clathrin-mediated endocytosis, cell signalling, apoptosis and cytoskeleton rearrangements.Its expression is characterized by multiple alternative splicing.Alternative promoter usage additional way to create diversity flexibility the regulation of expression.The aim this study was identify possible promoters ITSN1 gene.Methods.In silico prediction, 5¢ RACE, RT-PCR reporter assay were used for...
Transplantation of placenta-derived multipotent cells (PDMCs) is a promising approach for cell therapy to treat inflammation-associated colon diseases. However, the effect PDMCs on cancer remains unknown. The aim present study was characterize obtained from human (hPDMCs) and rat (rPDMCs) placentas evaluate their impact progression in rats. were by tissue explant culturing. Stemness- trophoblast-related gene expression studied using reverse transcription-polymerase chain reaction (RT-PCR),...
Keywords: Wheat streak mosaic virus, Triticum aestivum, phylogenetic analysis, sequencing, coat protein
Adaptor/scaffold proteins of the intersectin (ITSN) family are important components endocytic and signalling complexes.They coordinate trafficking events with actin cytoskeleton rearrangements modulate activity a variety pathways.In this review, we present our results as part recent findings on function ITSNs, role alternative splicing in generation ITSN1 diversity potential relevance ITSNs for neurodegenerative diseases cancer.
Âè ÿâ
Aim. Latent membrane protein 2A (LMP2A) of Epstein-Barr virus is implicated in the regulation viral latency.The aim current study was to identify proteins interacting with proline-rich motifs LMP2A.Methods.In silico prediction Scansite allowed recognize amphiphysin 1 (Amph1) as a binding partner LMP2A.Molecular cloning techniques, site-directed mutagenesis, vitro assay made it possible interaction interface Amph1/LMP2A complex.Sequential centrifugation steps were used isolate an exosomal...
We have shown NMDAR expression in cell transformation process at early stages of cancer, specifically HCC. The aim our study was to define the disease from precancerous liver towards cancer progression when subunits were expressed/detected. A fibrosis/HCC rat model, immunohistochemistry combined with epifluorescence microscopy imaging, Western blotting used. dynamics appearance subunits, their and methylation status during development HCC discussed.
Aim. Activation of receptor tyrosine kinases (RTK) by corresponding ligands results not only in signalpropagation, but also initiates a number processes, such as clathrin-mediated endocytosis, which precisely regulate biological outcome.These processes are tightly controlled coordinated action plethora proteins -enzymes, scaffolds and inhibitory molecules.An example an endocytic accessory protein that functions cell signaling is provided intersectin 1 (ITSN1).Previously we have shown ITSN1...
The purpose of this study was to investigate the immunophenotypes and gene expression profile high proliferative placenta-derived multipotent cells (PDMCs) population at different stages culture. We demonstrated that colonies resulting from single were either positive or negative for CK7, whereas only PDMC clones with weak CK7 (CK7low-clones) highly proliferative. Interestingly, vimentin (Vim+) placental stromal mesenchymal did not express in situ, but double CK7+Vim+ detection tissue...
The chronic consumption of mycotoxin-contaminated forage tends to the weight loss young cattle, decreasing reproductive success adult animals, milk yield, and tolerance high temperatures. aim this paper was study eff ects SNP C994G in CYP3A28 gene on productive characteristics dairy beef breeds. Cows were measured by body dynamics, exterior, reproduction, production traits, bone mineral density, bulls analyzed estimating their progeny traits. Dairy cows received dietary betaine supplement...