A5002623833- Genomic variations and chromosomal abnormalities
- Hereditary Neurological Disorders
- Cancer Genomics and Diagnostics
- Genetics and Neurodevelopmental Disorders
- SARS-CoV-2 and COVID-19 Research
- Chromosomal and Genetic Variations
- Neurological diseases and metabolism
- Genomics and Rare Diseases
- Genetic Neurodegenerative Diseases
- Glioma Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- Microtubule and mitosis dynamics
- Nutrition, Genetics, and Disease
- Immune responses and vaccinations
- Colorectal Cancer Treatments and Studies
- Building materials and conservation
- Cancer, Lipids, and Metabolism
- Neurogenetic and Muscular Disorders Research
- interferon and immune responses
- Genetics, Bioinformatics, and Biomedical Research
- Muscle Physiology and Disorders
- Thermal Regulation in Medicine
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Adrenal Hormones and Disorders
Institute of Molecular Biology and Genetics
2009-2024
National Academy of Sciences of Ukraine
2019-2022
International Clinic of Rehabilitation
2022
of our study was to identify and characterize the SARS-CoV-2 variants in COVID-19 patients' samples collected from different regions Ukraine determine relationship between phylogenetics epidemiology.
Background: Prostate cancer is one of the most common male cancers in Western countries and takes third place morbidity Ukraine. It a highly heterogeneous disease. Aim: To analyze relative expression levels TGFB1, IL1B, FOS, EFNA5, TAGLN, PLAU, EPDR1 genes malignant non-malignant prostate tissues. Materials Methods: Total RNA was isolated from 16 adenomas, 37 adenocarcinomas, 29 conventionally normal gene quantitative real-time polymerase chain reaction performed. Results: The significant...
Microbial activity following invasion of human-made structures and artifacts can have profound social economic consequences including the permanent loss cultural heritage. The unique frescoes in 11th century Saint Sophia's Cathedral (Kyiv, Ukraine) recently suffered from dark-spot biodeterioration. aim this work was to elucidate microbial nature biodeterioration biogeochemical processes occurring areas dark spots. Culture-independent approaches scanning electron microscopy with energy...
The study aimed to identify the clinically relevant gene variants in colon adenocarcinoma samples of Ukrainian patients using NGS Comprehensive Cancer Panel (CCP) implement them conveniently clinical practice. Methods. We have studied 20 with colorectal adenocarcinomas various differentiation grades. To variants, CCP data were filtered Franklin by Genoox database. Results. A total 79 variant alterations (SNVs, INDELs) found 28 409 genes. largest number mutations was 3 genes, APC, TP53, and...
We describe 2 Ukrainian families with unbalanced reciprocal translocations (RTs) involving the distal part of chromosome 10q. In both families, fathers were healthy carriers RT. Two affected patients from first family had an ∼2.3-Mb loss at 10q26.3 and ∼25-Mb gain 2q35qter, patient other ∼12.5-Mb 5p15.2pter ∼18-Mb 10q25.3q26.3. assume that intellectual disability (ID) in association congenital anomalies observed our was result cumulative effect gains losses chromosomal regions involved each...
The summarized results of 25-year studies department human genomics IMBG NASU are presented.The investigations were focused on identification molecular genetic nature genome coding and non-coding region mutations (genetic polymorphisms) rearrangements, their spectrum, origin in Ukrainian population.The role heterogeneity some severe monogenic complex disorder pathogenesis has been shown.The data concerning correlation between certain determinator gene phenotypical manifestation most common...
The aim of the study is to create a panel informative STR-markers in HLA locus for analysis genomic rearrangements different types malignant tumors as well selection matched sibling allogeneic transplantation.Methods.The preliminary screening six presumed located proximal, central and distal parts chromosome region (6p21.31)was performed by agarose gel electrophoresis PCR-fragments.The accurate genotyping most promising was carried out electrophoretic separation PCR-fragments polyacrylamide...
Aim.To identify and characterize the SARS-CoV-2 variants, collected upon new wave of COVID-19 outbreak in Ivano-Frankivs'k region Ukraine, using whole genome genotyping.Methods.The parallel sequencing was performed on processed RNA, isolated from nasopharyngeal swabs 19 patients, an Ion GeneStudio S5 Plus System.Results.All identified genotypes were referred to 20I/501Y.V1 clade, variant VUI202012/01 GRY (the B.1.1.7 lineage).In analyzed virus variants forty-seven various mutations...
Prader-Willi (PWS) and Angelman (AS) syndromes are two clinically distinct genetic diseases associated with multiple physical cognitive abnormalities.The cause of PWS AS is the alteration in 15q11.2-q13chromosomal region; expression genes this region subject to genome imprinting.Aim.To analyse frequency 15q11.2-q13rearrangements epigenetic alterations group Ukrainian patients AS.Methods.The methylation status SNRPN gene was analyzed by methylation-specific PCR (MS-PCR).Results.The absence...
ASSOCIATION OF THE EPHA1 AND PARP1 GENES POLYMORPHISMS WITH ALZHEIMER’S DISEASE
THE IMPACT OF SKEWED X-INACTIVATION IN SPINAL MUSCULAR ATROPHY PHENOTYPE MODIFICATION
DIFFERNTIAL DIAGNOSIS OF DELETION AND UNIPARENTAL DISOMY IN 15Q11.2-Q13 LOCUS
Abstract The rearrangements of the 15q11-q13 region such as deletions, duplications, uniparental disomies (UPDs), and translocations caused different neurodevelopment phenotypes including Prader-Willy Angelman syndromes, 5q11-q13 duplication syndrome, autism spectrum disorders. aim our study is to elaborate on most universal, convenient, informative molecular test for rapid detection alterations in patients with suspected 15q associated Eight short tandem repeats (STR) markers within...
Corona virus disease (COVID-19) is an infectious caused by the SARS-CoV-2 and its mutants. COVID-19 often cause of development pathologies on part respiratory system against background hypoxia. One alternative methods eliminating hypoxia carboxytherapy, since carbon dioxide (CO2) has anti-hypoxic, antioxidant effect due to physiological properties. The use CO2 in treatment can be effective symptoms pleuropulmonary pathology, as anti-inflammatory, antiviral antimicrobial effects.
The success of immunotherapy for ovarian cancer is determined by the effectiveness patient's immune response, as well qualitative and quantitative representation antigens main histocompatibility complex in tumor cells microenvironment.The level HLA (Human Leukocyte Antigens) genes expression tissue does not always correctly reflect these different populations cells.Aim.To analyze frequency range somatic deletions duplications locus.Methods.LOH-analysis (Loss Heterozyhosity) STR-markers...