A5084076358- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Radiopharmaceutical Chemistry and Applications
- Protein purification and stability
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Prostate Cancer Treatment and Research
- Marine and coastal ecosystems
- Aquatic Ecosystems and Phytoplankton Dynamics
- Galectins and Cancer Biology
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Biocrusts and Microbial Ecology
- Extracellular vesicles in disease
- Estrogen and related hormone effects
- Atherosclerosis and Cardiovascular Diseases
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Biosensors and Analytical Detection
- Biotin and Related Studies
- Mass Spectrometry Techniques and Applications
University of Turku
2015-2024
Turku Centre for Biotechnology
2011-2021
University of Helsinki
2008
Karolinska Institutet
1996
SARS-CoV-2 antibody detection assays are crucial for gathering seroepidemiological information and monitoring the sustainability of response against virus. The Spike protein’s receptor-binding domain (RBD) is a very specific target anti-SARS-CoV-2 antibodies detection. Moreover, many neutralizing mapped to this domain, linking RBD with potential. Detection IgG antibodies, rather than IgM or total likely play larger role in understanding antibody-mediated protection vaccine response. Here we...
A broad-spectrum noncompetitive immunoassay allowing sensitive and simple detection of a group similar compounds would be an ideal tool for screening low-molecular weight analytes (<2000 Da) having many variants. However, the development essential antibody pair capable sandwich-type recognition analytes' small generic core structure is demanding task due to limited space available simultaneous binding two different antibodies. We report here assay cyanobacterial microcystins (MCs) nodularins...
Cancer antigen 15–3 (CA15-3) is widely utilized for monitoring metastatic breast cancer (BC). However, its utility early detection of severely limited due to poor clinical sensitivity and specificity. The glycosylation CA15-3 known be affected by BC, therefore it might offer a way construct glycovariant assays with improved To this end, we performed lectin-based glycoprofiling BC-associated CA15-3. expressed BC cell line was immobilized on microtitration wells using an anti-CA15-3 antibody....
Abstract Cancer antigen 125 (CA125) is a widely used biomarker in monitoring of epithelial ovarian cancer (EOC). Due to insufficient specificity CA125, its diagnostic use severely compromised. Abnormal glycosylation CA125 unique feature cells and could improve differential diagnosis the disease. Here we describe development quantitative lateral flow immunoassay (LFIA) aberrantly glycosylated which superior conventional (CA125IA). With 30 min read-out time, LFIA showed 72% sensitivity, at 98%...
Owing to the high carcinogenicity of aflatoxins, these toxic secondary metabolites pose a severe risk human and animal health can have major economic implications. Herein, we report development noncompetitive immunoassay for aflatoxins based on monoclonal capture antibody unique anti-immunocomplex (anti-IC) fragment (scFv) isolated from synthetic repertoire. The anti-IC scFv recognizes immunocomplex enables sandwich-type assays despite small size analyte. single-step assay developed in this...
Abstract Adhesive pili assembled through the chaperone–usher pathway are hair-like appendages that mediate host tissue colonization and biofilm formation of Gram-negative bacteria 1–3 . Archaic pili, most diverse widespread adhesins, promising vaccine drug targets owing to their prevalence in troublesome multidrug-resistant pathogens 1,4,5 However, architecture assembly–secretion process remain unknown. Here, we present cryo-electron microscopy structure prototypical archaic Csu pilus...
ABSTRACT The mercury-sensing regulatory protein, MerR (Tn 21 ), which regulates mercury resistance operons in Gram-negative bacteria, was subjected to directed evolution an effort generate a mutant that responds Cd but not Hg. Oligonucleotide-directed mutagenesis used introduce random mutations into the key metal-binding regions of MerR. effects these were assessed using vector controlled expression green fluorescent protein (GFP) and luciferase via mer operator/promoter. An Escherichia coli...
We have developed switchable lanthanide luminescence-based binary probe technology for homogeneous detection of avidin, which is a tetrameric protein. Two different nonluminescent label moieties—a light-absorbing antenna ligand and ion carrier chelate—were conjugated to separate biotins, known as avidin's natural ligand. The assay was based on binding the two differently labeled biotins sites target protein consequent self-assembly luminescent complex from moieties. Specific luminescence...
In directed evolution experiments, a single randomization scheme of an antibody gene does not provide optimal diversity for recognition all sizes antigens. this study, we have expanded the potential our universal library, termed ScFvP, with second distinct diversification scheme. ScFvM, was designed closer to center antigen binding site in same framework as earlier. Also, CDR-H3 loop structures were redesigned be shorter, 5–12 aa and mostly without canonical salt bridge between Arg106H...
Affinity maturation is often applied to improve the properties of antibodies isolated from universal antibody libraries in vitro. A synthetic human scFv library was constructed single immunoglobulin framework enable rapid affinity by updated Kunkel's mutagenesis. The initial diversity generated predominantly VH domain combined with only 36 VL variants yielding 3 × 1010 unique members phage-displayed library. After three rounds panning enriched genes primary selections against lysozyme were...
Abstract The analysis of extracellular vesicles (EVs) typically requires tedious and time-consuming isolation process from bio-fluids. We developed a nanoparticle-based time resolved fluorescence immunoassay (NP-TRFIA) that uses biotinylated antibodies against the proteins tetraspanin family tumor-associated antigens for capturing EVs urine samples cell culture supernatants without need isolation. captured-EVs were detected either with Eu 3+ -chelate or -doped labels conjugated to...
Abstract Background Mammalian display is an appealing technology for therapeutic antibody development. Despite the advantages of mammalian display, such as full-length IgG with glycosylation and its inherent ability to select antibodies good biophysical properties, restricted library size large culture volumes remain challenges. Bxb1 serine integrase commonly used stable genomic integration genes into cells, but presently lacks efficiency required libraries. To increase integrase-mediated...
Sulfa antibiotics (sulfonamides) are a group of molecules sharing the p-aminobenzenesulfonamide moiety. Sulfonamides used in veterinary and human medicine. Sometimes, meat or milk medicated animals is contaminated with residual sulfonamides. Current analytical methods for sulfonamides unfit screening food, because they either too laborious, insensitive, specific few sulfa compounds only. A rapid immunoassay detection all sulfas single reaction would thus be useful. Previously, we protein...
The study of biomolecular interactions is at the heart biomedical research. Fluorescence and Förster resonance energy transfer (FRET) are potent versatile tools in studying these interactions. Fluorescent proteins enable genetic encoding which facilitates their use recombinant protein vivo applications. To eliminate autofluorescence background encountered applications based on fluorescent proteins, lanthanide labels can be used as donor fluorophores. Their long emission lifetime enables...
Measurement of serum cancer antigen 125 (CA125) is the standard approach for epithelial ovarian (EOC) diagnostics and follow-up. However, clinical specificity not optimal because increased values are also detected in healthy controls benign diseases. CA125 known to be differentially glycosylated EOC, potentially offering a way construct assays with improved specificity. Our goal was identify carbohydrate-reactive lectins discriminating between originating from EOC noncancerous sources.CA125...
Targeting the androgen receptor (AR) pathway prolongs survival in patients with prostate cancer, but resistance rapidly develops. Understanding this is confounded by a lack of noninvasive means to assess AR activity vivo. We report intracellular accumulation secreted antigen-targeted antibody (SATA) that can be used characterize disease, guide therapy, and monitor response. AR-regulated human kallikrein-related peptidase 2 (free hK2) tissue-specific antigen produced cancer...
We have developed a homogeneous assay method in which the lanthanide ion carrier and light absorbing components of luminescent chelate are separated two distinct molecules that can together form mixed complex. The label moieties were conjugated to oligonucleotides used as probes detect complementary target DNA. background signal was very low, indicating highly dependent on hybridization adjacent positions oligonucleotide.
Förster resonance energy transfer (FRET) is a powerful tool in studying biomolecular interactions. Intrinsically fluorescent lanthanide chelates are increasingly used as FRET donors due to their long emission lifetime that enables the use of time resolution. Fluorescent proteins, on other hand, owe popularity intrinsic luminescent properties, facilitating fusion proteins. In this investigation, two pairs, terbium(III) chelate with green protein (GFP) and europium(III) yellow (YFP), were...