A5070826746- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Bacterial Genetics and Biotechnology
- Enzyme Production and Characterization
- Proteins in Food Systems
- Protein Hydrolysis and Bioactive Peptides
- Hormonal and reproductive studies
- Infant Nutrition and Health
- Protein purification and stability
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cell Adhesion Molecules Research
- Drug-Induced Adverse Reactions
- Prostate Cancer Treatment and Research
- Sperm and Testicular Function
- Food Allergy and Anaphylaxis Research
- Diabetes Management and Education
- Synthesis and Biological Evaluation
- CNS Lymphoma Diagnosis and Treatment
- Probiotics and Fermented Foods
- Complement system in diseases
- Drug Transport and Resistance Mechanisms
- Pancreatic function and diabetes
- Organic Chemistry Synthesis Methods
- Amino Acid Enzymes and Metabolism
- Diabetes Management and Research
Karolinska Institutet
2024
University of Turku
1988-2011
Turku Centre for Biotechnology
1995-2011
University of Maryland, College Park
1997
Agricultural Research Service
1997
Beltsville Agricultural Research Center
1997
University of California, Berkeley
1988
Donnan potential (interior negative) across the outer membrane of Escherichia coli was measured by distribution [14C]choline in a mutant with deletion through genes for active transport choline. Calculation showed that presence membrane-derived oligosaccharides periplasm could quantitatively explain magnitude and periplasmic volume. By measuring permeability porin channels intact cells suspended solutions widely different ionic strengths, it shown changing from 5 mV to approximately 100 had...
We have recently described a monoclonal antibody (mAb) 4G4 recognizing 70-kD molecule constitutively expressed on human endothelial cells and subpopulations of lymphocytes. showed that this molecule, which we named lymphocyte-vascular adhesion protein 2 (L-VAP-2), mediates lymphocyte to cultured cells. Protein sequencing tryptic peptides from immunoaffinity-purified L-VAP-2 revealed sequence identity between CD73 (ecto-5'-nucleotidase, E.C.3.1.3.5), COS transfected with cDNA were positively...
Affinity maturation is often applied to improve the properties of antibodies isolated from universal antibody libraries in vitro. A synthetic human scFv library was constructed single immunoglobulin framework enable rapid affinity by updated Kunkel's mutagenesis. The initial diversity generated predominantly VH domain combined with only 36 VL variants yielding 3 × 1010 unique members phage-displayed library. After three rounds panning enriched genes primary selections against lysozyme were...
An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme active against a wide range aryl-beta-glucosides beta-linked disaccharides. structural gene for cloned in Escherichia coli. consisted an open reading frame 1,350 bp encoding protein 450 amino acids with calculated M(r) 51,303. exhibited 45 to 66% identity five bacterial beta-glucosidases.
Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence determined, and gene cloned as two separate fragments. DNA sequencing showed an open reading frame 432 acids (M(r) 47,439) exhibiting moderately low homology with AspATs from other sources. Sequence alignment enzyme chicken mitochondrial, cytoplasmic Escherichia coli performed MULTALIN program further...
Introduction: The treatment of patients with hematological malignancies and acute arterial thrombosis (ATE) is challenging due to the risk bleeding complications during treatment. Data on incidence factors for ATE in this group are very limited. Aims: We aimed evaluate leukemia (AL) lymphoid malignancies. Material Methods: Patients (AL), malignancies, diagnosed following cancer diagnosis, who were treated followed at Department Hematology, Karolinska University Hospital, 2005–2020,...
Abstract Cyclodextrins have diverse, important applications in food industry. These are based on the ability of cyclodextrins to form molecular complexes with small—molecular compounds. harmless oral administration and advantageous effects by achieved simple mixing small amounts them material being stabilized. Development convenient cost‐effective processes for production undergoing there promises near future obtaining which realistic price be used foods. While been more than ten years Japan...
Abstract Objective To purify complement component C3 from bovine serum, characterize and analyze NH 2 -terminal amino acid sequences its various cleavage products, do cross-species homology comparisons. Animals healthy lactating Holstein cows, adult female New Zealand White rabbits. Procedure Bovine was isolated cleaved to C3b. The resulting protein analyzed determine apparent molecular mass of segments. Bands were electroblotted onto a membrane excised, then determined. Results preparation...