A5088279242- Fungal and yeast genetics research
- Muscle metabolism and nutrition
- Cardiovascular and exercise physiology
- Microbial Metabolic Engineering and Bioproduction
- Fermentation and Sensory Analysis
- Yeasts and Rust Fungi Studies
- Genetic Neurodegenerative Diseases
- Bacteriophages and microbial interactions
- Amino Acid Enzymes and Metabolism
- Photosynthetic Processes and Mechanisms
- Cancer Research and Treatments
- Virus-based gene therapy research
- Polyamine Metabolism and Applications
- Advanced biosensing and bioanalysis techniques
- Biopolymer Synthesis and Applications
- Microbial Fuel Cells and Bioremediation
- DNA Repair Mechanisms
- Biotin and Related Studies
- Exercise and Physiological Responses
- Advanced Nanomaterials in Catalysis
- Nanoplatforms for cancer theranostics
- Connective tissue disorders research
- Muscle Physiology and Disorders
- Heart Rate Variability and Autonomic Control
- Porphyrin Metabolism and Disorders
Lviv State University of Physical Culture
2014-2024
National Academy of Agrarian Sciences of Ukraine
2022
Institute of Cell Biology
2005-2020
National Academy of Sciences of Ukraine
2000-2020
Institute of Molecular Biology and Genetics
2005-2014
National Institute of Diabetes and Digestive and Kidney Diseases
2002
National Institutes of Health
2002
Palladin Institute of Biochemistry
1999-2000
All-Russian Scientific Research Institute of Food Biotechnology
1996
Creatine plays a vital role in the normal metabolism of central nervous system, heart, bones, and muscle tissues. It is one most extensively used dietary supplements worldwide. critical energy metabolism, functioning as source high-energy phosphate groups that facilitate fast recycling ADP into ATP within cells, particularly during stressed states. The biosynthesis creatine requires essential substrates arginine S-adenosylmethionine, which can influence regulation human body. acts negative...
Background To study the peculiarities of changes in creatinine concentration blood serum untrained men during prolonged usage training loads different volume and intensity, to determine value this biochemical marker for assessment adaptive body fitness training. Methods We examined 50 aged 18–20 years who had no contraindications practicing fitness. Taking into account aim research, we divided these people two groups: group A B. The research participants used load regimes intensity:...
Abstract Iron deficiency causes oversynthesis of riboflavin in several yeast species, known as flavinogenic yeasts. However, the mechanisms such regulation are not known. We found that mutations causing overproduction and iron hyperaccumulation ( rib80, rib81 hit1 ), well cobalt excess or all provoke oxidative stress Pichia guilliermondii yeast. content cells, production both malondialdehyde by P. wild type mutant strains depend on a carbon source used cultivation media. The data suggest...
Pichia guilliermondii is a representative of group so-called flavinogenic yeast species that overproduce riboflavin (vitamin B(2)) in response to iron limitation. Using insertion mutagenesis, we isolated P. mutants overproducing riboflavin. Analysis nucleotide sequence recombination sites revealed cassettes integrated into the genome disrupting genes similar VMA1 gene Ashbya gossypii and Saccharomyces cerevisiae FES1 FRA1 S. cerevisiae. The constructed guilliermondiiΔvma1-17 mutant possessed...
It is known for many years that iron represses synthesis of riboflavin (RF) and most RF-synthesizing enzymes in several yeast species, as flavinogenic yeasts. However, the mechanism such repression not known. We have found transcription RIB1 RIB7 genes coding first last RF biosynthesis model organism Pichia guilliermondii. To decipher molecular mechanisms iron-dependent repression, isolation study regulatory mutants defective corresponding regulation desirable. no suitable methods were...
This paper presents an up-to-date review of research data on the specific features lactic acid metabolism and its role as effector vital regulatory mechanisms. Lactic is alpha-hydroxy monocarboxylic acid. Physical loads submaximal intensity some diseases can cause dramatic increase content in body fluids. The excessive lactate removed from working muscle either metabolized by other tissues or excreted human body. Alteration lactate-pyruvate balance one main markers development cardiac...
Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence determined, and gene cloned as two separate fragments. DNA sequencing showed an open reading frame 432 acids (M(r) 47,439) exhibiting moderately low homology with AspATs from other sources. Sequence alignment enzyme chicken mitochondrial, cytoplasmic Escherichia coli performed MULTALIN program further...
The purpose of the article: to carry out a theoretical analysis scientific literature data on disorders connective tissue metabolism, in particular dysplasia, which can cause increased injuries and other negative health consequences when performing physical activity various types. article briefly describes changes structural elements metabolism tissue, lead disruption its basic functions. More often, such changes, according literature, are described by term “connective dysplasia”...
We report the development of amperometric biosensors (ABSs) employing flavocytochrome
Abstract The codA gene of Corynebacterium glutamicum PCM 1945 coding for a creatinine deiminase (CDI) (EC 3.5.4.21) has been amplified and cloned. recombinant strain Escherichia coli that overproduces the (His) 6 ‐tagged inactive CDI C. as inclusion bodies constructed. After solubilization in presence 0.3% N ‐lauroylsarcosine, enzyme was renaturated purified by single‐step procedure using metal‐affinity chromatography. yield is ~30 mg from 1 L culture. sufficiently stable under conditions...