A5078698421- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Metal Extraction and Bioleaching
- Viral gastroenteritis research and epidemiology
- RNA regulation and disease
- Extraction and Separation Processes
- Minerals Flotation and Separation Techniques
- SARS-CoV-2 and COVID-19 Research
- Mine drainage and remediation techniques
- RNA modifications and cancer
- Infectious Encephalopathies and Encephalitis
- Animal Virus Infections Studies
- Genomics and Phylogenetic Studies
- Neutropenia and Cancer Infections
- Nuclear Structure and Function
- Microbial Community Ecology and Physiology
- Methemoglobinemia and Tumor Lysis Syndrome
- Neonatal Health and Biochemistry
Case Western Reserve University
2020-2024
The University of Texas Health Science Center at Houston
2022
Palmetto Hematology Oncology
2022
CSIRO Land and Water
2005-2007
Health Sciences and Nutrition
2006
Seth Research Foundation
2006
Abstract The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights need for coordinated research to combat COVID-19. A particularly important aspect is development of medication. In addition viral proteins, structured RNA elements represent a potent alternative as drug targets. search drugs that target requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, worldwide consortium NMR researchers aims...
Small-molecule targeting of RNA stem loops in 5′-end SARS-CoV-2 reveals novel targets for viral inhibition.
Abstract Nuclear export of influenza A virus (IAV) mRNAs occurs through the nuclear pore complex (NPC). Using Auxin-Induced Degron (AID) system to rapidly degrade proteins, we show that among nucleoporins localized at nucleoplasmic side NPC, TPR is key nucleoporin required for mRNAs. recruits TR anscription and EX port (TREX)−2 NPC exporting a subset cellular By degrading components TREX-2 (GANP, Germinal-center Associated Protein; PCID2, PCI domain containing 2), require replication....
A novel, extremely thermoacidophilic, obligately chemolithotrophic archaeon (strain JP7 T ) was isolated from a solfatara on Lihir Island, Papua New Guinea. Cells of this organism were non-motile, Gram-negative staining, irregular-shaped cocci, 0.5–1.5 μm in size, that grew aerobically by oxidation sulfur, Fe 2+ or mineral sulfides. anaerobically using 3+ as terminal electron acceptor and H 2 S an donor but did not oxidize hydrogen with elemental sulfur acceptor. Strain optimally at 74 °C...
Selective pressures on viruses provide opportunities to establish target site specificity and mechanisms of antivirals. Enterovirus (EV)-A71 with resistant mutations in the stem loop (SL) II internal ribosome entry (IRES) (SLII resist ) were selected at low doses antiviral dimethylamiloride (DMA)-135. The EV-A71 mutants DMA-135 concentrations that inhibit replication wild-type virus. IRES structures harboring induced efficient expression Luciferase messenger RNA presence noncytotoxic...
Abstract Selective pressures on positive-strand RNA viruses provide opportunities to establish target site specificity and mechanisms of action antivirals. Here, Enterovirus-A71 revertant with resistant mutations in the SLII IRES domain (SLII resist ) were selected at low doses antiviral DMA-135. The EV-A71 DMA-135 concentrations that robustly inhibit replication wild-type virus. structures harboring suppressor induced efficient expression reporter Luciferase mRNA presence non-cytotoxic...
The SARS-CoV-2 pandemic, and the likelihood of future coronavirus pandemics, has rendered our understanding biology more essential than ever. Small molecule chemical probes offer to both reveal novel aspects virus replication serve as leads for antiviral therapeutic development. RNA-biased amiloride scaffold was recently tuned target a viral RNA structure critical translation in enterovirus 71, ultimately uncovering mechanism modulate positive-sense replication. Analysis CoV genomes many...
Viruses pose a great threat to people’s lives. Enterovirus A71 (EV-A71) infects children and infants all over the world with no FDA-approved treatment date. Understanding basic mechanisms of viral processes aids in selecting more efficient drug targets designing effective antivirals thwart this virus. The 5´-untranslated region (5’-UTR) RNA genome is composed cloverleaf structure an internal ribosome entry site (IRES). Cellular proteins that bind regulate replication, while those IRES also...
As early as 166 AD, biotechnology was applied to the extraction of metals from ores in copper mines Cyprus, and 1928 Kennecott, USA, ?dump leaching? ? use microorganisms extract low grade mine waste material conducted on commercial scale. It not until 1947 that Colmer Hinkle 1 demonstrated role play oxidation mineral sulfides for release solution. Currently, 20% annual global production results largely through bioleaching chalcocite (Cu2S). Many other metals, such gold, cobalt, nickel,...
Positive Strand RNA (PSR) viruses, such as coronaviruses and enteroviruses, cause serious health economic threats worldwide, currently seen with the COVID-19 pandemic. This has drawn attention to importance of identifying new antivirals molecular targets in viruses. The multifunctionality PSR genomes make them desirable for therapeutic intervention. Here, we present a class that can inhibit SARS-CoV-2 replication vitro by targeting conserved viral structures at 5'-end. Specifically, stem...
Emerging evidence suggests that patients with listed drug allergy have inferior outcomes than without allergies.Unverified penicillin can lead to utilizing less efficacious antibiotics, increased length/cost of hospital stay, and adverse effects including antibiotic resistance Clostridium difficile infections.Reports seldom reflect tested allergy.~0.2% undergo testing are truly allergic the medication.This project's first aim was assess prevalence label in Lyndon B. Johnson Hospital patient...