Jaakko A. Puhakka

ORCID: 0000-0001-9423-6556
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About
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Research Areas
  • Metal Extraction and Bioleaching
  • Mine drainage and remediation techniques
  • Minerals Flotation and Separation Techniques
  • Anaerobic Digestion and Biogas Production
  • Wastewater Treatment and Nitrogen Removal
  • Microbial bioremediation and biosurfactants
  • Microbial Fuel Cells and Bioremediation
  • Biofuel production and bioconversion
  • Extraction and Separation Processes
  • Microbial Community Ecology and Physiology
  • Environmental remediation with nanomaterials
  • Microbial Metabolic Engineering and Bioproduction
  • Water Treatment and Disinfection
  • Chromium effects and bioremediation
  • Electrochemical sensors and biosensors
  • Phosphorus and nutrient management
  • Toxic Organic Pollutants Impact
  • Supercapacitor Materials and Fabrication
  • Algal biology and biofuel production
  • Mineral Processing and Grinding
  • Groundwater flow and contamination studies
  • Enhanced Oil Recovery Techniques
  • Marine and coastal ecosystems
  • Membrane Separation Technologies
  • Constructed Wetlands for Wastewater Treatment

Tampere University
2014-2023

Tampere University of Applied Sciences
1990-2015

Geological Survey of Finland
2009

Outokumpu (Finland)
2009

University of Oulu
2001-2008

University of Akureyri
2007

Feng Chia University
2007

Tampere University
2007

CSIRO Land and Water
2002-2004

Commonwealth Scientific and Industrial Research Organisation
2002

Abstract Biological sulfate reduction is increasingly replacing chemical unit processes in mining biotechnology. Sulfate reducing bacteria (SRB) can be used for treating ground‐ and surface waters contaminated with acid mine drainage (AMD), recovering metals from wastewater process streams. Biologically produced H 2 S precipitates as metal sulfides, while biogenic bicarbonate alkalinity neutralizes acidic waters. This paper reviews various passive active SRB‐based alternatives well some...

10.1002/elsc.200720216 article EN Engineering in Life Sciences 2007-12-01

Automated image analysis software, CellC, was developed and validated for quantification of bacterial cells from digital microscope images. CellC enables automated enumeration cells, comparison total count specific images [e.g., 4',6-diamino-2-phenylindole (DAPI) fluorescence in situ hybridization (FISH) images], provides quantitative estimates cell morphology. The software includes an intuitive graphical user interface that easy usage as well sequential multiple without intervention....

10.2144/000112018 article EN BioTechniques 2005-12-01

Microalgae are a promising feedstock for biofuel and bioenergy production due to their high photosynthetic efficiencies, growth rates no need external organic carbon supply. In this study, utilization of Chlorella vulgaris (a fresh water microalga) Dunaliella tertiolecta marine biomass was tested as anaerobic H2 CH4 production.Anaerobic serum bottle assays were conducted at 37°C with enrichment cultures derived from municipal digester sludge. Low levels produced by cultures, but subsequently...

10.1186/1754-6834-4-34 article EN cc-by Biotechnology for Biofuels 2011-01-01

Abstract The effects of hydraulic retention time (HRT) and sulfide toxicity on ethanol acetate utilization were studied in a sulfate‐reducing fluidized‐bed reactor (FBR) treating acidic metal‐containing wastewater. HRT determined with continuous flow FBR experiments. percentage oxidation was 99.9% even at 6.5 h (loading 2.6 g L −1 d ), while accumulated the HRTs below 12 1.4 ). Partial accompanied by decreased concentrations dissolved (DS) alkalinity effluent, eventually resulted process...

10.1002/bit.20061 article EN Biotechnology and Bioengineering 2004-04-06

A methanogenic fluidized bed reactor (FBR) fed with lactate and tetrachloroethene (PCE) was operated for 14 months to study the effect of electron donor PCE loading on chloroethene dechlorination rates. Lactate continu ously at 200 mg/L (2.2 mmol/L), influent feed concentration increased stepwise from 3.5 160 μmol/L. Vinyl chloride (VC) ethene accounted 80% 20%, respectively, dechlorination. Batch tests various donors showed that H2, propionate, supported PCE, trichloroethene (TCE),...

10.1021/es9606539 article EN Environmental Science & Technology 1997-05-29

Bacterial diversity of lactate- and ethanol-utilizing sulfate-reducing fluidized-bed reactor (FBR) communities was investigated with culture-independent methods. The FBRs were fed for 500 days synthetic mineral processing wastewater containing sulfate, zinc iron hydraulic retention time 16-24 h. Sodium lactate or ethanol used as electron donor microbial sulfate reduction. For characterization, 16S rRNA gene clone libraries denaturing gradient gel electrophoresis (DGGE) fingerprinting...

10.1016/s0168-6496(03)00284-8 article EN FEMS Microbiology Ecology 2004-01-13

ABSTRACT A high-rate fluidized-bed bioreactor has been treating polychlorophenol-contaminated groundwater in southern Finland at 5 to 8°C for over 6 years. We examined the microbial diversity of using three 16S ribosomal DNA (rDNA)-based methods: denaturing gradient gel electrophoresis, length heterogeneity-PCR analysis, and restriction fragment polymorphism analysis. The molecular study revealed that process was dependent on a stable bacterial community with low species diversity. dominant...

10.1128/aem.68.1.173-180.2002 article EN Applied and Environmental Microbiology 2002-01-01

Abstract Microbial community composition dynamics was studied during H 2 fermentation from glucose in a fluidised‐bed bioreactor (FBR) aiming at obtaining insight into the microbiology and factors resulting instability of biofilm processes. FBR production performance characterised by an instable pattern prompt onset followed rapid decrease. Gradual enrichment organisms increased diversity attached suspended‐growth phase bacterial communities operation. bacteria included potential producers,...

10.1002/bit.21299 article EN Biotechnology and Bioengineering 2006-12-13

10.1016/j.ijhydene.2010.04.174 article EN International Journal of Hydrogen Energy 2010-06-14

Summary The aim of this study was to test three flat plate photobioreactor configurations for growth Chlorella vulgaris under non‐axenic conditions and characterize quantify associated bacterial communities. cultivations were conducted using tap water‐based media introduce background population. Growth algae monitored over time with independent methods. Additionally, the quantity quality eukaryotes bacteria analysed culture‐independent molecular tools based on denaturing gradient gel...

10.1111/j.1751-7915.2011.00298.x article EN other-oa Microbial Biotechnology 2011-09-21
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