A5024945543- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Protein Structure and Dynamics
- Synthesis and Characterization of Heterocyclic Compounds
- Advanced biosensing and bioanalysis techniques
- Advanced NMR Techniques and Applications
- Enzyme Structure and Function
- Microbial Natural Products and Biosynthesis
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Computational Drug Discovery Methods
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- RNA Research and Splicing
- Synthesis and biological activity
- Synthetic Organic Chemistry Methods
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Click Chemistry and Applications
- Catalytic Cross-Coupling Reactions
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Chemical synthesis and alkaloids
- ATP Synthase and ATPases Research
Goethe University Frankfurt
2016-2025
Osnabrück University
2014-2024
Institute of Organic Chemistry
2024
National Renewable Energy Laboratory
2024
University of Colorado Boulder
2024
Leibniz-Institute for New Materials
2024
Environmental Energy & Engineering
2024
The University of Adelaide
2024
Imperial College London
2024
Fraunhofer Institute for Translational Medicine and Pharmacology
2024
Abstract Many cellular functions rely on DNA-binding proteins finding and associating to specific sites in the genome. Yet mechanisms underlying target search remain poorly understood, especially case of highly organized mammalian cell nucleus. Using as a model Tet repressors (TetRs) searching for multi-array locus, we quantitatively analyse process human cells with single-molecule tracking single-cell protein–DNA association measurements. We find that TetRs explore nucleus reach their by 3D...
Abstract The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights need for coordinated research to combat COVID-19. A particularly important aspect is development of medication. In addition viral proteins, structured RNA elements represent a potent alternative as drug targets. search drugs that target requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, worldwide consortium NMR researchers aims...
Homodimeric class I cytokine receptors are assumed to exist as preformed dimers that activated by ligand-induced conformational changes. We quantified the dimerization of three prototypic in plasma membrane living cells single-molecule fluorescence microscopy. Spatial and spatiotemporal correlation individual receptor subunits showed revealed associated Janus kinase 2 (JAK2) dimerizes through its pseudokinase domain. Oncogenic hyperactive JAK2 mutants promoted ligand-independent...
We present a high-resolution nuclear magnetic resonance (NMR) solution structure of 14-mer RNA hairpin capped by cUUCGg tetraloop. This short and very stable presents an important model system for the study dynamics using NMR spectroscopy, molecular (MD) simulations force-field development. The extraordinary high precision (root mean square deviation 0.3 A) could be achieved measuring incorporating all currently accessible parameters, including distances derived from Overhauser effect (NOE)...
Herein we describe the synthesis of negatively charged N-heterocyclic carbene (NHC)-functionalized palladium and gold nanoparticles (NPs), which are stable in water for over 3 months. The formation these NHC–NPs proceeds via an efficient ligand exchange procedure. This method was successfully applied to different NHCs bearing sulfonate carboxylate groups. obtained PdNPs were investigated as catalysts hydrogenation reactions showed high catalytic activity (TON up 2500 TOF 2000 h–1).
The homogeneous catalyzed dehydrogenation of methanol in a synthetically valuable cross-coupling reaction was achieved. By the use simple ruthenium-N-heterocyclic carbene complex, MeOH and primary or secondary amines can be converted into formamides.
N-heterocyclic carbenes (NHCs) represent a leading class of ligands in organometallic chemistry, but have been rarely exploited as stabilizers for metal nanoparticles (NPs). We report the first example NHC stabilized Pd-NPs that demonstrate long term stability. These were synthesized by facile ligand exchange protocol using rationally designed chained NHCs (LC-NHCs). Furthermore, we surface modification results significant chemoselectivity model reaction.
While detailed information on the nanoscale-organization of proteins within intracellular membranes has emerged from electron microcopy, their spatiotemporal dynamics is scarce. By use a photostable rhodamine attached specifically to Halo-tagged in mitochondrial membranes, we were able track and localize single protein complexes such as Tom20 ATP synthase suborganellar structures live cells. Individual membrane inner outer mitochondria imaged over long time periods with localization...
Stress granules (SGs) are cytosolic, nonmembranous RNA–protein complexes. In vitro experiments suggested that they formed by liquid–liquid phase separation; however, their properties in mammalian cells remain unclear. We analyzed the distribution and dynamics of two paradigmatic RNA-binding proteins (RBPs), Ras GTPase-activating protein SH3-domain–binding (G3BP1) insulin-like growth factor II mRNA-binding 1 (IMP1), with single-molecule resolution living neuronal cells. Both RBPs exhibited...
Type I interferons (IFNs) activate differential cellular responses through a shared cell surface receptor composed of the two subunits, IFNAR1 and IFNAR2. We propose here mechanistic model for how IFN plasticity is regulated on level dimerization. Quantitative single-molecule imaging assembly in plasma membrane living cells clearly identified IFN-induced dimerization The negative feedback regulator ubiquitin-specific protease 18 (USP18) potently interferes with recruitment into ternary...
SARS-CoV-2 contains a positive single-stranded RNA genome of approximately 30 000 nucleotides. Within this genome, 15 elements were identified as conserved between SARS-CoV and SARS-CoV-2. By nuclear magnetic resonance (NMR) spectroscopy, we previously determined that these fold independently, in line with data from vivo ex-vivo structural probing experiments. These contain non-base-paired regions potentially harbor ligand-binding pockets. Here, performed an NMR-based screening poised...
Abstract Microorganisms contribute to the biology and physiology of eukaryotic hosts affect other organisms through natural products. Xenorhabdus Photorhabdus ( XP ) living in mutualistic symbiosis with entomopathogenic nematodes generate products mediate bacteria–nematode–insect interactions. However, a lack systematic analysis biosynthetic gene clusters (BGCs) has limited understanding how interactions between organisms. Here we combine pangenome sequence similarity networks analyse BGCs...
Localization and tracking of individual receptors by single-molecule imaging opens unique possibilities to unravel the assembly dynamics signaling complexes in plasma membrane. We present a comprehensive workflow for analyzing receptor diffusion interaction live cells at single molecule level with up four colors. Two engineered, monomeric GFP variants, which are orthogonally recognized anti-GFP nanobodies, employed efficient selective labeling target proteins membrane photostable...
Riboswitches are highly structured RNA elements that control the expression of many bacterial genes by binding directly to small metabolite molecules with high specificity and affinity. In Bacillus subtilis , two classes riboswitches have been described discriminate between guanine adenine despite an extremely degree homology both in their primary secondary structure. We identified intermolecular base triples purine ligands respective riboswitch RNAs NMR spectroscopy. Here, is mediated...
G-quadruplex structures can be formed at the single-stranded overhang of telomeric DNA, and ligands able to stabilize this structure have recently been identified as potential anticancer drugs. Among binders, we studied binding ability berberine sanguinarine, two members alkaloid family, an important class natural products long known for medicinal purpose. Our spectroscopic (CD, NMR, fluorescence) studies molecular modeling approaches revealed modes ligand–complex stoichiometries >1:1 ligand...
In living color: Efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super-resolution imaging in live cells is described. The dynamics cellular nanostructures at plasma membrane were monitored time resolution few seconds. combination dual-color FPALM imaging, submicroscopic receptor organization within context skeleton was resolved (see figure). Detailed facts importance to specialist readers are published as "Supporting Information"....