A5028384006- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Monoclonal and Polyclonal Antibodies Research
- Supramolecular Self-Assembly in Materials
- Neuroinflammation and Neurodegeneration Mechanisms
- Enzyme Structure and Function
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Ion channel regulation and function
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Cannabis and Cannabinoid Research
- Advanced Biosensing Techniques and Applications
- Protein purification and stability
- Diabetes Management and Research
- Zebrafish Biomedical Research Applications
- Polyamine Metabolism and Applications
- Cancer, Hypoxia, and Metabolism
- Neuroscience of respiration and sleep
- Chemical Synthesis and Analysis
- Retinal Development and Disorders
- Antifungal resistance and susceptibility
- Neonatal and fetal brain pathology
- Nanofabrication and Lithography Techniques
- Diet and metabolism studies
Universitat Ramon Llull
2021-2023
Institut Químic de Sarrià
2021-2023
Sapienza University of Rome
2018-2021
University of Bologna
1989-2004
University of Ferrara
1997
Instituto Gulbenkian de Ciência
1996
Azienda Unita' Sanitaria Locale Di Modena
1990
The widespread expression of many therapeutic targets in both diseased and healthy tissues presents a significant challenge for protein therapeutics, often resulting dose-limiting side effects. To focus the action biotherapeutics at disease site, they can be reversibly inactivated by tethering an affinity mask through linker cleavable disease-specific cue. However, current methods to generate masks require extensive screening masking sequence libraries. Here, we report workflow design first...
Abstract: In rat hippocampal synaptosomes, adenosine decreased the K + (15 m M ) or kainate (1 evoked release of glutamate and aspartate. An even more pronounced effect was observed in presence stable analogue, R ‐phenylisopropyladenosine. All these effects were reversed by selective A 1 receptor antagonist 8‐cyclo‐pentyltheophylline. same synaptosomal preparation, (30 strongly stimulated preloaded [ 3 H]adenosine a partially Ca 2+ ‐dependent tetrodotoxin (TTX)‐sensitive manner. Moreover,...
Cancer cells reprogramme one‐carbon metabolism ( OCM ) to sustain growth and proliferation. Depending on cell demands, serine hydroxymethyltransferase SHMT dynamically changes the fluxes of by reversibly converting tetrahydrofolate THF into 5,10‐methylene‐ glycine. is a tetrameric enzyme that mainly exists in three isoforms; two localize cytosol 1/ 2α) one 2) mitochondria. Both cytosolic isoforms can also translocate nucleus de novo thymidylate synthesis support Finally, expression levels...
BrainBikes, a new family of bicyclic brain shuttle peptides, can efficiently transport protein therapeutics across endothelium.
Pharmacological blockade of the (NMDA) receptor at critical stages brain development may have long-lasting effects on chemistry and animal behavior. We report here experiments in which competitive NMDA antagonist CGP 39551 was administered to rat pups from postnatal day 7 (P7) P18. The stage treatment selected primarily target cerebellum, whose granule cells undergo post-mitotic migration establishment synaptic connections during this period. focused our study long-term consequences neuronal...
De novo thymidylate synthesis is a crucial pathway for normal and cancer cells. Deoxythymidine monophosphate (dTMP) synthesized by the combined action of three enzymes: serine hydroxymethyltransferase (SHMT1), dihydrofolate reductase (DHFR) synthase (TYMS), with latter two being targets widely used chemotherapeutics such as antifolates 5-fluorouracil. These proteins translocate to nucleus after SUMOylation are suggested assemble in this compartment into complex. We report intracellular...
ABSTRACT De novo thymidylate synthesis is a crucial pathway for normal and cancer cells. Deoxythymidine monophosphate (dTMP) synthesized by the combined action of three enzymes: serine hydroxymethyltransferase (SHMT), dihydrofolate reductase (DHFR) synthase (TYMS), latter two targets widely used chemotherapeutics such as antifolates 5-fluorouracil. These proteins translocate to nucleus after SUMOylation are suggested assemble in this compartment into complex (dTMP-SC). We report...
Antibody therapeutics show great potential to treat a variety of diseases. Often, the dose that can be safely administered is limited by side effects arise from interaction with target outside diseased tissue. Conditionally-active antibodies provide an additional layer selectivity improve safety. Distinct external stimuli or internal cues enable different control strategies and applications. However, current antibody masking have low transferability across stimuli. Here we propose versatile...