A5018652598- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Mesenchymal stem cell research
- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- PI3K/AKT/mTOR signaling in cancer
- Platelet Disorders and Treatments
- Immunodeficiency and Autoimmune Disorders
- Escherichia coli research studies
- Tissue Engineering and Regenerative Medicine
- Acute Myeloid Leukemia Research
- Blood groups and transfusion
- Viral gastroenteritis research and epidemiology
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hematopoietic Stem Cell Transplantation
- Eosinophilic Disorders and Syndromes
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Lung Cancer Treatments and Mutations
- Pluripotent Stem Cells Research
- T-cell and Retrovirus Studies
- Complement system in diseases
- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
Humanitas University
2025
IRCCS Humanitas Research Hospital
2019-2025
Policlinico S.Orsola-Malpighi
2014-2023
University of Rome Tor Vergata
2023
Azienda USL di Bologna
2009-2023
Azienda Sanitaria Universitaria Integrata di Trieste
2022-2023
University of L'Aquila
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2001-2021
Azienda Ospedaliero-Universitaria Careggi
2021
University of Florence
2018-2020
CTLA-4 (CD152) is a cell surface receptor that behaves as negative regulator of the proliferation and effector function T cells. We have previously shown also expressed on neoplastic lymphoid myeloid cells, it can be targeted to induce apoptosis. In our study, we extended analysis discovered expression detectable by flow cytometry 30 34 (88%) lines derived from variety human malignant solid tumors including carcinoma, melanoma, neuroblastoma, rhabdomyosarcoma osteosarcoma (but not in primary...
Recent findings have highlighted that constitutively active phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling is a common feature T-cell acute lymphoblastic leukemia (T-ALL), where it upregulates cell proliferation, survival, and drug resistance. These observations lend compelling weight to the application PI3K/Akt/mTOR inhibitors in therapy T-ALL. Here, we analyzed therapeutic potential novel dual PI3K/mTOR inhibitor NVP-BEZ235, an orally bioavailable...
Platelet-rich plasma (PRP) is used clinically in liquid or gel form to promote tissue repair.Because of the poor mechanical properties, conventional PRP often difficult handle when clinical settings and requires secure implantation a specific site, otherwise released growth factors could be washed out during an operation.In this study, we analyzed end product recently developed commercially available system (FIBRINET ® ), which dense pliable, platelet-rich fibrin matrix (PRFM).We...
Mesenchymal stem cells (MSCs) have been derived from multiple sources of the horse including umbilical cord blood (UCB) and amnion. This work aimed to identify characterize equine amniotic fluid (AF), CB Wharton's Jelly (WJ). Samples were obtained 13 mares at labour. AF isolated by centrifugation, while WJ was prepared incubating with an enzymatic solution for 2 h. All cell lines cultured in DMEM/TCM199 plus fetal bovine serum. Fibroblast-like observed 7/10 (70%) AF, 6/8 (75%) 8/12 (66.7%)...
Treatment with alemtuzumab has resulted in negative responses for minimal residual disease (MRD) patients chronic lymphocytic leukemia (CLL). In a prior analysis we demonstrated that it is possible to achieved MRD negativity, as assessed by polyclonality of immunoglobulin heavy chain after consolidation alemtuzumab. This phase II study evaluated 34 CLL who received an effort improve the quality their response fludarabine-based induction. Subsequent peripheral blood stem-cell (PBSC)...
The clinical use of endothelial progenitor cells is hampered by difficulties in obtaining an adequate number functional progenitors. This study aimed to establish whether human thoracic aortas harvested from healthy multiorgan donors can be a valuable source angiogenic Immunohistochemical tissue studies showed that two distinct cell populations with putative stem capabilities, one composed CD34+ and the other c-kit+ cells, are present between media adventitia aortas. Ki-67+ high growth...
Recent investigations have documented that constitutively activated phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling is a common feature T-cell acute lymphoblastic leukemia (T-ALL), where it strongly influences growth and survival. These findings lend compelling weight for the application PI3K/Akt/mTOR inhibitors in T-ALL. However, our knowledge T-ALL limited not clear whether could be an effective innovative therapeutic strategies. Here, we analyzed...
Human mesenchymal stromal (stem) cells (hMSCs) isolated from adult bone marrow (BM-hMSCs) as well amnion (AM-hMSCs) and chorion (CM-hMSCs) term placenta leaves were studied by transmission electron microscopy (TEM) to investigate their ultrastructural basic phenotype. At flow cytometry, the showed a homogeneous expression of markers commonly used identify hMSCs, i.e., CD105, CD44, CD90, CD166, HLA-ABC positivities, CD45, AC133, HLA-DR negativities. However, TEM revealed subtle yet...
Dental pulp is a heterogeneous microenviroment where unipotent progenitor and pluripotent mesenchymal stem cells cohabit. In this study we investigated whether human Pulp Stromal (Stem) Cells (DP-SCs) committed to the angiogenic fate. DP-SCs showed specific immunophenotypical profile positive for CD29, CD44, CD73, CD105, CD166 negative CD14, CD34, CD45, in accordance with that reported bone marrow-derived SCs. The Oct-4 expression DP-SCs, evaluated through RT-PCR analysis, increased relation...
Abstract BACKGROUND: The combination of fludarabine, cyclophosphamide, and rituximab (FCR) has produced promising results in chronic lymphocytic leukemia other lymphoproliferative disorders. authors report the final from a multicenter, prospective study examining FCR Waldenstrom macroglobulinemia (WM). METHODS: Forty‐three patients with symptomatic WM that was untreated or pretreated 1 line chemotherapy received 375 mg/m 2 intravenously on day fludarabine 25 cyclophosphamide 250 days through...
Background Persistent mixed chimerism represents a state in which recipient and donor cells stably co-exist after hematopoietic stem cell transplantation. However, since most of the studies reported literature engraftment was observed nucleated cells, this study we determined origin mature erythrocytes patients with persistent transplantation for hemoglobinopathies. Results were compared singly picked out burst-forming unit – erythroid colonies collected from peripheral blood bone...
Abstract Hemolytic uremic syndrome (HUS) caused by intestinal Shiga toxin–producing Escherichia coli infections is a worldwide health problem, as dramatically exemplified the German outbreak occurred in summer 2011 and constant burden of cases children. toxins (Stx) play pivotal role HUS triggering endothelial damage kidney brain through globotriaosylceramide (Gb3Cer) receptor targeting. Moreover, Stx interact with human neutrophils, experimentally demonstrated vitro observed patients HUS. A...
Vaccination coverage against COVID-19 among health care workers (HCWs) of the University Health Agency Giuliano-Isontina (ASUGI) Trieste (North-eastern Italy) by 1 January 2022 was 90.4% with at least one vaccine dose, 84.9% 2 doses, and 75.1% 3 98.2% Comirnaty (Pfizer BioNtech, New York, NY, USA) versus 1.8% Spikevax (Moderna, Cambridge, MA, USA). From October 2020 to 7 February 2022, 1652 SARS-CoV-2 infections were notified in HCWs ASUGI Trieste. Although overall risk contagion increased...
Abstract To potentiate the response of acute myelogenous leukemia (AML) cells to tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) cytotoxicity, we have examined efficacy a combination with perifosine, novel phosphatidylinositol-3-kinase (PI3K)/Akt signaling inhibitor. The rationale for using such is that perifosine was recently described increase TRAIL-R2 receptor expression and decrease cellular FLICE-inhibitory protein (cFLIP) in human lung cancer cell lines. Perifosine...
Apolipoprotein A1 mimetic peptide (D-4F), synthesized from D-amino acid, enhances the ability of high-density lipoprotein to protect low-density (LDL) against oxidation in atherosclerotic disease. Using a rat model type I diabetes, we investigated whether chronic use D-4F would lead up-regulation heme oxygenase (HO)-1, endothelial cell marker (CD31<sup>+</sup>), and thrombomodulin (TM) expression increase number progenitor cells (EPCs). Sprague-Dawley rats were rendered diabetic with...