A5022310925- Escherichia coli research studies
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Immune Cell Function and Interaction
- Vibrio bacteria research studies
- Cancer, Hypoxia, and Metabolism
- DNA Repair Mechanisms
- Glycosylation and Glycoproteins Research
- Complement system in diseases
- RNA Interference and Gene Delivery
- Muscle metabolism and nutrition
- Cancer Research and Treatments
- RNA Research and Splicing
- Plant Genetic and Mutation Studies
- Clostridium difficile and Clostridium perfringens research
- Cytomegalovirus and herpesvirus research
- Enterobacteriaceae and Cronobacter Research
- Advanced biosensing and bioanalysis techniques
- Microbial Metabolism and Applications
- Mycotoxins in Agriculture and Food
- Biosensors and Analytical Detection
University of Bologna
2012-2024
Institute of Experimental Physics of the Slovak Academy of Sciences
2016
Inserm
2015
Robert Bosch (Germany)
2011
Policlinico S.Orsola-Malpighi
2010
Medica (Italy)
2006
Società Italiana di Nefrologia
2006
In up to 60% of human cancers, p53 gene mutations are responsible for direct inactivation the tumor suppressor function p53. Alternative mechanisms described thus far mainly affect its posttranslational regulation. X-linked dyskeratosis congenita, a multisystemic syndrome characterized by increased cancer susceptibility, DKC1 encoding dyskerin cause selective defect in translation subgroup internal ribosome entry site (IRES)-containing cellular mRNAs. this study, we show that impairment can...
Abstract Hemolytic uremic syndrome (HUS) caused by intestinal Shiga toxin–producing Escherichia coli infections is a worldwide health problem, as dramatically exemplified the German outbreak occurred in summer 2011 and constant burden of cases children. toxins (Stx) play pivotal role HUS triggering endothelial damage kidney brain through globotriaosylceramide (Gb3Cer) receptor targeting. Moreover, Stx interact with human neutrophils, experimentally demonstrated vitro observed patients HUS. A...
Intestinal infections with Shiga toxin-producing Escherichia coli (STEC) in children can lead to the hemolytic uremic syndrome (HUS). toxins (Stx) released gut by bacteria enter blood stream and target kidney causing endothelial injury. Free have never been detected of HUS patients, but they found on surface polymorphonuclear leukocytes (PMN).With respect their clinical features, relevance amounts serum Stx (cytotoxicity assay human cells) PMN-bound (cytofluorimetric assay) 46 patients...
Dyskerin is a pseudouridine (ψ) synthase involved in fundamental cellular processes including uridine modification rRNA and small nuclear RNA telomere stabilization. functions are altered X-linked dyskeratosis congenita (X-DC) cancer. Dyskerin's role pseudouridylation has been suggested to underlie the alterations mRNA translation described cells lacking dyskerin function, although relevant direct evidences currently lacking. Our purpose was establish definitely whether defective function...
The interest in the development of nanoscale plasmonic technologies has dramatically increased recent years. photonic properties nanopatterns can be controlled and tuned via their size, shape, or arrangement constituents. In this work, we propose a 2D hybrid metallic polymeric nanostructure based on octupolar framework with enhanced sensing property. We analyze its features both numerically experimentally, demonstrating higher values relevant figures merit: estimated surface-enhanced Raman...
Exposure of C2C12 muscle cells to hypertonic stress induced an increase in cell content creatine transporter mRNA and transport activity, which peaked after about 24 h incubation at 0.45 osmol (kg H 2 O) −1 . This induction activity was prevented by addition either cycloheximide, inhibit protein synthesis, or actinomycin D, RNA synthesis. Creatine uptake these is largely Na + dependent kinetic analysis revealed that its under conditions resulted from V max the ‐dependent component, with no...
Abstract Human intestinal infections by Shiga toxin (Stx)-producing Escherichia coli cause hemorrhagic colitis and hemolytic uremic syndrome (HUS), which represents the main of acute renal failure in early childhood. In HUS, Stx released gut enter bloodstream are targeted to endothelium. The mechanism delivery is still a matter debate, although role polymorphonuclear leukocytes (PMN) as carrier has been indicated. aim this paper was better define interactions between human PMN. Direct...
Abstract Intoxicated endothelial cells release pro-inflammatory cytokines involved in renal damage; amount of inversely correlates with the toxin carried by PMN. The damage induced Stx represents main pathogenic event HUS associated STEC infections humans. Stx, released gut bacteria, enter bloodstream and are targeted to endothelia. role PMN as a carrier has been object controversy. In this paper, we confirm binding Stx1 PMN, also showing its degranulating effects on full-loaded leukocytes,...
To mimic what might happen in cells exposed to hypertonicity, the effects of increased osmolarity and ionic strength on cell-free protein synthesis have been examined. Translation globin mRNA by rabbit reticulocyte lysate decreased 30—60% when osmolality was from 0.35 0.53osmol/kg water addition NaCl, KCl, CH3CO2Na or CH3CO2K. In contrast, equivalent additions compatible osmolytes betaine myo-inositol caused a 40—50% increase rate translation, whereas amino acids (50—135mM) that are...
Hemolytic-uremic syndrome, the main cause of acute renal failure in early childhood, is caused primarily by intestinal infections from some Escherichia coli strains that produce Shiga toxins. The toxins released gut are targeted to endothelium after binding polymorphonuclear leukocytes. presence feces and circulating neutrophils 20 children with hemolytic uremic syndrome was evaluated Vero cell cytotoxicity assay flow cytometric analysis, respectively. latter showed on leukocytes 13...
Abstract Background Hemolytic uremic syndrome (HUS) is the main cause of acute renal failure in early childhood. Most cases are due to intestinal infections from Escherichia coli strains (STEC) which produce by Shiga toxin (Stxs). Stx1 and Stx2 produced STEC gut absorbed into circulation and, after binding on polymorphonuclear leukocytes (PMNs), targeted endothelium. The aim present work was development a method detect Stxs bound circulating PMNs diagnose patients with HUS. Methods White...
It has been known that ribosome‐inactivating proteins (RIPs) from plants damage ribosomes by removing adenine a precise position of rRNA. Subsequently it was observed all tested RIPs depurinate DNA, and some them also non‐ribosomal RNAs poly(A), hence the denomination polynucleotide glycosylases proposed. We report now ricin, saporin‐L2, saporin‐S6, gelonin momordin poly(ADP‐ribosyl)ated poly(ADP‐ribose) polymerase (auto modified enzyme), an enzyme involved in DNA repair. but induce...
Hemolytic uremic syndrome (eHUS) is a severe complication of human infections with Shiga toxins (Stxs)-producing Escherichia coli. A key step in the pathogenesis eHUS interaction Stxs blood components before targeting renal endothelial cells. Here, we show that single proteolytic cleavage Stx2a A-subunit, resulting into two fragments (A1 and A2) linked by disulfide bridge (cleaved Stx2a), dictates different binding abilities. Uncleaved was confirmed to bind neutrophils trigger...
Treatment of human endothelial cells with Shiga toxin 1 and 2 leads to the upregulation genes encoding proinflammatory molecules involved in pathogenesis hemolytic-uremic syndrome. The paradoxical effect inhibitors mRNA translation, such as toxins, that at same time induce protein expression was investigated by studying relationship between their enzymatic activity (abstraction adenine from nucleic acids) induction interleukin-8 granulocyte-macrophage colony-stimulating factor cells. As a...
The main cause of acute renal failure in children is HUS (haemolytic uraemic syndrome), a consequence intestinal infections with Escherichia coli strains producing Stx (Shiga toxins). released the gut by non-invasive bacteria reach bloodstream and are targeted to cerebral endothelium triggering HUS. PMN (polymorphonuclear leucocytes) seem be involved delivery through an unidentified membrane receptor (Kd=10⁻⁸ M; 2×10⁵ binding sites) which does not allow internalization. Some experts field...
Hemolytic uremic syndrome (HUS), the leading cause of acute renal failure in children (< 3 years), is mainly related to Shiga toxins (Stx)-producing Escherichia coli (STEC) infections. STEC are confined gut resulting hemorrhagic colitis, whereas Stx delivered blood target kidney and brain, with unclear mechanisms, triggering HUS 5 15% infected children. were found on circulating cells, free sera (soluble Stx) or cell-derived microvesicles (particulate Stx), whereby relationship between these...
Shiga toxins (Stx) play an important role in the pathogenesis of hemolytic uremic syndrome, a life-threatening renal sequela human intestinal infection caused by specific Escherichia coli strains. Stx target restricted subset endothelial cells that possess globotriaosylceramide receptor, like glomeruli. The toxins, composed five B chains and single enzymatic A chain, removing adenines from ribosomes DNA, trigger apoptosis production pro-inflammatory cytokines cells. Because bacteria are...
Inactivation of Artemia salina and rabbit ribosomes by gelonin requires ATP a high-Mr factor present in the reticulocyte-lysate post-ribosomal supernatant. The kinetic constants gelonin-catalysed release adenine from A. are Km = 4.35 microM Kcat. 0.1 min-1 absence cofactors, 1.15 108 their presence. last two values similar to those measured for ricin A chain cofactors (Km 2.02 317 min-1).