A5069412131- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- Epigenetics and DNA Methylation
- Analytical Chemistry and Chromatography
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Fungal and yeast genetics research
- Microfluidic and Capillary Electrophoresis Applications
- Cancer-related gene regulation
- Fungal Biology and Applications
- Mass Spectrometry Techniques and Applications
- Histone Deacetylase Inhibitors Research
- Environmental Toxicology and Ecotoxicology
- Marine Ecology and Invasive Species
- Advanced Proteomics Techniques and Applications
- Metabolism and Genetic Disorders
- Trace Elements in Health
- Polyamine Metabolism and Applications
- Endometriosis Research and Treatment
Innsbruck Medical University
2015-2024
Universität Innsbruck
2015-2024
Vienna Biocenter
2014
Institute of Medical Microbiology and Hygiene
2010
University of Münster
2010
Linköping University
2006
Weizmann Institute of Science
1971-1980
Five endosomal sorting complexes required for transport (ESCRTs) mediate the degradation of ubiquitinated membrane proteins via multivesicular bodies (MVBs) in lysosomes. ESCRT-0, -I, and –II interact with cargo on endosomes. ESCRT-II also initiates assembly a ringlike ESCRT-III filament consisting Vps20, Snf7, Vps24, Vps2. The AAA–adenosine triphosphatase Vps4 disassembles recycles complex, thereby terminating ESCRT pathway. A mechanistic role intraluminal vesicle (ILV) formation has been...
Nucleotide modifications within RNA transcripts are found in every organism all three domains of life. 6-methyladeonsine (m6A), 5-methylcytosine (m5C) and pseudouridine (Ψ) highly abundant nucleotide coding sequences eukaryal mRNAs, while m5C m6A have also been discovered archaeal bacterial mRNAs. Employing vitro translation assays, we systematically investigated the influence on translation. We introduced m5C, m6A, Ψ or 2′-O-methylated nucleotides at each positions a codon ErmCL mRNA...
The transcription factor FOXO3 is associated with poor outcome in high-stage neuroblastoma (NB), as it facilitates chemoprotection and tumor angiogenesis. In other entities, stimulates metastasis formation, one of the biggest challenges treatment aggressive NB. However, impact on metastatic potential neuronal cells remains largely unknown. present study, we uncover small leucine-rich proteoglycan family member lumican (LUM) a FOXO3-regulated gene that cellular migration By drug-library...
Structure of human mTORC1 components The (mechanistic target rapamycin complex 1) garners much attention as a signaling hub that coordinates input from growth-factor receptors and nutrient availability with metabolism cell growth proliferation. de Araujo et al. report the crystal structure LAMTOR (or “Ragulator”) helps assemble at lysosomal membrane for activation. functional studies reveal how LAMTOR1 wraps around other subunits to hold them in place interacts Rag guanosine triphosphatases...
The degradation and recycling of cellular components is essential for cell growth survival. Here we show how selective non-selective lysosomal protein pathways cooperate to ensure survival upon nutrient limitation. A quantitative analysis starvation-induced proteome remodeling in yeast reveals comprehensive changes already the first three hours. In this period, many different integral plasma membrane proteins undergo endocytosis vacuoles via multivesicular body (MVB) pathway. Their becomes...
Signaling from lysosomes controls cellular clearance and energy metabolism. Lysosomal malfunction has been implicated in several pathologies, including neurodegeneration, cancer, infection, immunodeficiency, obesity. Interestingly, many functions are dependent on the organelle position. motility requires integration of extracellular intracellular signals that converge a competition between motor proteins ultimately control lysosomal movement microtubules. Here, we identify novel upstream...
Significance Cardiolipins are a unique class of phospholipids in mitochondrial membranes that crucial for cellular bioenergetics as they stabilize respiratory chain complexes. In contrast to most other phospholipids, cardiolipins substituted with four, rather than only two fatty acyl side chains. Consequently, this opens up vast number different theoretically possible molecular lipid species. Experimentally assessing the diversity cardiolipin species is analytically challenging. study we...
Cellular homeostasis requires the ubiquitin-dependent degradation of membrane proteins. This was assumed to be mediated exclusively either by endoplasmic reticulum-associated (ERAD) or endosomal sorting complexes required for transport (ESCRT)-dependent lysosomal degradation. We identified in Saccharomyces cerevisiae an additional pathway that selectively extracts proteins at Golgi and endosomes cytosolic proteasomes. One endogenous substrate this endosome Golgi-associated (EGAD) is...
Cardiolipin (CL) is a phospholipid specific for mitochondrial membranes and crucial many core tasks of this organelle. Its acyl chain configurations are tissue specific, functionally important, generated via post-biosynthetic remodeling. However, process lacks the necessary specificity to explain CL diversity, which especially evident highly compositions in mammalian tissues. To investigate so far elusive regulatory origin homeostasis mice, we combine lipidomics, integrative transcriptomics,...
Significance The transcription factor nuclear kappa B (NF-κB) plays a vital role in cellular immune, stress, and proliferative responses by regulating genes. To maintain correct execution of the genetic program to prevent pathologies (e.g., chronic inflammation), activation NF-κB has be transient precisely controlled. We describe unique inhibitory mechanism that mediates NF-κB–dependent regulation inflammation-associated genes uses arginine methylation RelA subunit protein methyltransferase...
Abstract Ergothioneine (EGT; 2-mercaptohistidine trimethylbetaine) is a trimethylated and sulphurised histidine derivative which exhibits antioxidant properties. Here we report that deletion of Aspergillus fumigatus egtA (AFUA_2G15650), encodes trimodular enzyme, abrogated EGT biosynthesis in this opportunistic pathogen. biosynthetic deficiency A. significantly reduced resistance to elevated H 2 O menadione, respectively, impaired gliotoxin production resulted attenuated conidiation....
Capillary zone electrophoresis-mass spectrometry (CE-MS) is a mature analytical tool for the efficient profiling of (highly) polar and ionizable compounds. However, use CE-MS in comparison to other separation techniques remains underrepresented metabolomics, as this approach still perceived technically challenging less reproducible, notably migration time. The latter key reliable metabolic profiles unknown biomarker identification that complementary high resolution MS/MS. In work, we present...
Alkylglycerol monooxygenase (glyceryl-ether monooxygenase, EC 1.14.16.5) is the only enzyme known to cleave O-alkyl bond of ether lipids which are essential components brain membranes, protect eye from cataract, interfere or mediate signalling processes, and required for spermatogenesis. Along with phenylalanine hydroxylase, tyrosine tryptophan nitric oxide synthase, alkylglycerol one five enzymatic reactions depend on tetrahydrobiopterin. Although first described in 1964, no sequence had...
Context: Previous studies have implicated a deficiency in the inflammatory response women who develop endometriosis. The specific immunological deficits not been completely elucidated.
Iron is an essential element for all eukaryotes but its excess has deleterious effects. Aspergillus fumigatus produces extracellular siderophores iron uptake and the intracellular siderophore ferricrocin (FC) distribution storage of iron. previously been shown to increase content ferric FC expression putative vacuolar importer CccA (AFUA_4G12530), indicating a role both vacuole in detoxification. In this study, we show that CccA-deficiency decreases resistance particular combination with...
The flatworm Macrostomum lignano features a duo-gland adhesive system that allows it to repeatedly attach and release from substrates in seawater within minute. However, little is known about the molecules involved this temporary adhesion. In study, we show attachment of M. relies on secretion two large proteins, adhesion protein 1 (Mlig-ap1) Mlig-ap2. We revealed both proteins are expressed gland cells their distribution footprints was spatially restricted. RNA interference knockdown...
Increased understanding of the role nanomaterial protein corona in driving uptake into, and impacts on, cells organisms, consequent need for characterization corona, has led to a flourishing methods isolation analysis constituent proteins over past decade. However, despite 700 studies date, very little is understood terms which provide most precise comprehensive corona. With increasing importance modeling formation its correlation with biological impacts, it timely properly characterize...
RNA modifications are crucial factors for efficient protein synthesis. All classes of RNAs that involved in translation modified to different extents. Recently, mRNA and their impact on gene regulation became a focus interest because they can exert variety effects the fate mRNAs. within coding sequences either directly or indirectly interfere with In order investigate roles various natural occurring nucleotides, we site-specifically introduced them into sequence reporter mRNAs subsequently...
In vivo phosphorylation of the five histone H1 variants H1a−H1e including H10 in NIH 3T3 mouse fibroblasts was examined during cell cycle by using a combination HPLC techniques and conventional AU gel electrophoresis. Phosphorylation starts late G1 phase increases throughout S phase. phase, exist as molecules containing 0 or 1 (H1a, H1c), 0−2 (H1d), 0−3 (H1b, H1e) phosphate groups with share unphosphorylated protein ranging between 35% 75%, according to particular subtype. Pulse−chase...
Iron is a micronutrient required by almost all living organisms, including fungi. Although this metal abundant, its bioavailability low either in aerobic environments or within mammalian hosts. As consequence, pathogenic microorganisms evolved high affinity iron acquisition mechanisms which include the production and uptake of siderophores. Here we investigated utilization these molecules species Paracoccidioides genus, causative agents systemic mycosis. It was demonstrated that starvation...
Abstract The primary cilium emanates from the cell surface of growth-arrested cells and plays a central role in vertebrate development tissue homeostasis. mechanisms that control ciliogenesis have been extensively explored. However, intersection between GPCR signaling ubiquitin pathway stability are unknown. Here we observe cAMP elevation promotes cilia resorption. At centriolar satellites, identify multimeric complex nucleated by PCM1 includes two kinases, NEK10 PKA, E3 ligase CHIP. We show...
Early diagnosis of invasive aspergillosis (IA) remains challenging, with available diagnostics being limited by inadequate sensitivities and specificities. Triacetylfusarinine C, a fungal siderophore that has been shown to accumulate in urine animal models, is potential new biomarker for IA.