A5055193163- Iron Metabolism and Disorders
- Genomics and Chromatin Dynamics
- Trace Elements in Health
- Nanoparticle-Based Drug Delivery
- Lanthanide and Transition Metal Complexes
- Escherichia coli research studies
- Drug Transport and Resistance Mechanisms
- Acute Myocardial Infarction Research
- Hemoglobinopathies and Related Disorders
- Ferroptosis and cancer prognosis
- Advanced MRI Techniques and Applications
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Coronary Interventions and Diagnostics
- Orthopaedic implants and arthroplasty
- Heme Oxygenase-1 and Carbon Monoxide
- Protein Interaction Studies and Fluorescence Analysis
- Natural Antidiabetic Agents Studies
- Selenium in Biological Systems
- X-ray Diffraction in Crystallography
- Cancer-related gene regulation
- Advanced Glycation End Products research
- Cancer Research and Treatments
- Dental Implant Techniques and Outcomes
- Viral gastroenteritis research and epidemiology
Innsbruck Medical University
2013-2024
Universität Innsbruck
2012-2024
Vienna Biocenter
2013
DKFZ-ZMBH Alliance
1989
German Cancer Research Center
1989
Nitric oxide (NO) generated by inducible NO synthase 2 (NOS2) affects cellular iron homeostasis, but the underlying molecular mechanisms and implications for NOS2-dependent pathogen control are incompletely understood. In this study, we found that up-regulated expression of ferroportin-1 (Fpn1), major exporter, in mouse human cells. Nos2(-/-) macrophages displayed increased content due to reduced Fpn1 allowed an enhanced acquisition intracellular bacterium Salmonella typhimurium. Nos2 gene...
Abstract In stimulating effector functions of mononuclear phagocytes, IFN‐γ is pivotal importance in host defense against intramacrophage pathogens including salmonellae. As the activity modulated by iron and since a sufficient availability essential for growth pathogens, we investigated regulatory effects on homeostasis immune function murine macrophages infected with Salmonella typhimurium . ‐infected caused significant reduction uptake via transferrin receptor 1 resulted an increased...
Methylation and acetylation of position-specific lysine residues in the N-terminal tail histones H3 H4 play an important role regulating chromatin structure function. In case H3-Lys(4), H3-Lys(9), H3-Lys(27), H4-Lys(20), degree methylation was variable from mono- to di- or trimethylated state, each which presumed be involved organization activation repression genes. Here we investigated interplay between histone H4-Lys(20) trim-ethylation at induced (beta-major/beta-minor glo-bin), repressed...
H1 histones, isolated from logarithmically growing and mitotically enriched human lymphoblastic T-cells (CCRF-CEM), were fractionated by reversed phase hydrophilic interaction liquid chromatography, subjected to enzymatic digestion, analyzed amino acid sequencing mass spectrometry. During interphase the four subtypes present in these cells differ their maximum phosphorylation levels: histone H1.5 is tri-, H1.4 di-, H1.3 H1.2, only monophosphorylated. The site-specific occurs exclusively on...
Numerous in vivo, vitro and clinical studies report on beneficial effects of strontium with respect to increased bone growth. Based this knowledge the aim study was evaluate early late osseointegration stages functionalized titanium implants showing sustained release (Sr) further investigate its potential systemic effect. Strontium (Ti-Sr-O) Grade 4 (Control) were inserted femoral condyle New Zealand White rabbits. The Ti-Sr-O coating characterized using Scanning Electron Microscopy (SEM)...
Macrophages are central for the immune control of intracellular microbes. Heme oxygenase 1 (HO-1, hmox) is first and rate limiting enzyme in breakdown heme originating from degraded senescent erythrocytes heme-proteins, yielding equal amounts iron, carbon monoxide biliverdin. HO-1 strongly up-regulated macrophages response to inflammatory signals, including bacterial endotoxin. In view essential role iron growth proliferation bacteria along with known effects metal on innate function, we...
Lipocalin-2 (Lcn2) is an innate immune peptide with pleiotropic effects. Lcn2 binds iron-laden bacterial siderophores, chemo-attracts neutrophils and has immunomodulatory apoptosis-regulating In this study, we show that upon infection Salmonella enterica serovar Typhimurium, promotes iron export from Salmonella-infected macrophages, which reduces cellular content enhances the generation of pro-inflammatory cytokines. represses IL-10 production while augmenting Nos2, TNF-α, IL-6 expression....
In vivo phosphorylation of the five histone H1 variants H1a−H1e including H10 in NIH 3T3 mouse fibroblasts was examined during cell cycle by using a combination HPLC techniques and conventional AU gel electrophoresis. Phosphorylation starts late G1 phase increases throughout S phase. phase, exist as molecules containing 0 or 1 (H1a, H1c), 0−2 (H1d), 0−3 (H1b, H1e) phosphate groups with share unphosphorylated protein ranging between 35% 75%, according to particular subtype. Pulse−chase...
Thyroid physiology is closely related to oxidative changes. The aim of this controlled study was evaluate the levels nutritional anti-oxidants such as vitamin C, zinc (Zn) and selenium (Se), investigate any association them with parameters thyroid function pathology including benign malignant diseases. This evaluation Se included a total 1401 subjects (1186 adults 215 children) distributed follows: control group (n = 687), disease (85 children 465 adults); (2 79 adults). Clinical patients...
Altered mitochondrial metabolism plays a pivotal role in the development and progression of various diseases, including cancer. Cell lines are frequently used as models to study (dys)function, but little is known about their respiration metabolic properties comparison primary tissue origin. We have developed method for assessment oxidative phosphorylation prostate samples only 2 mg wet weight using high‐resolution respirometry. Reliable protocols were established investigate respiratory...
Imbalances of iron and dopamine metabolism along with mitochondrial dysfunction have been linked to the pathogenesis Parkinson's disease (PD). We previously suggested a direct link between homeostasis metabolism, as can increase cellular uptake into macrophages thereby promoting oxidative stress responses. In this study, we investigated interplay iron, dopamine, activity in neuroblastoma SH-SY5Y cells human induced pluripotent stem cell (hiPSC)-derived dopaminergic neurons differentiated...
Posttranslational modification of histones is a common means regulating chromatin structure and thus diverse nuclear processes. Using hydrophilic interaction liquid chromatographic separation method in combination with mass spectrometric analysis, the present study investigated alterations histone H4 methylation/acetylation status interplay between methylation acetylation during vitro differentiation mouse erythroleukemia cells how these modifications affect structure. Independently type...
Neutrophil gelatinase-associated lipocalin (NGAL/Lipocalin-2/Lcn-2) is a 25kDa protein which involved in host defence against certain Gram negative bacteria upon binding of iron loaded bacterial siderophores thereby limiting the availability this essential nutrient to resulting inhibition their growth and pathogenicity. As important for intracellular bacterium Chlamydia pneumoniae we questioned whether Lcn-2 affects course infection. We employed primary peritoneal macrophages obtained from...
Zinc sequestration by macrophages is considered a crucial host defense strategy against infection the intracellular bacterium Salmonella enterica serovar Typhimurium. However, underlying mechanisms remain elusive. In this study, we found that zinc favors pathogen survival within macrophages. Salmonella-hosting contained higher free levels than did uninfected and cells successfully eliminated bacteria, which was paralleled impaired production of reactive oxygen species (ROS) nitrogen (RNS) in...
The current scientific literature is inconsistent regarding the potential beneficial or deleterious effects of high-intensity physical activities on pelvic floor (PF) in women. So far, it has not been established with certainty whether disparate breathing mechanisms may exert short- long-term influence PF function this context, although based physiological interrelationship activation, seems plausible.To propose a basic concept different patterns during strenuous efforts. Methodical...
The binding of all known linker histones, named H1a through H1e, including H10 and H1t, to a model chromatin complex based on DNA fragment containing the mouse mammary tumor virus long terminal repeat promotor was systematically studied. As for histone subtype H1b, we found dissociation constant 8–16 nm single mononucleosome (210 base pairs), whereas other subtypes varied between 2 4 nm. Most H1 namely H1a, H1c, H1d/e, H10, completely aggregate polynucleosomes (1.3 kilobase pairs, 6...
The opportunistic fungal pathogen Aspergillus fumigatus produces siderophores for uptake and storage of iron, which is essential its virulence. main precursor siderophore biosynthesis (SB), ornithine, can be produced from glutamate in the mitochondria or by cytosolic hydrolysis ornithine-derived arginine. Here, we studied impact mitochondrial versus ornithine on SB comparison arginine auxotrophic mutants ΔargEF ΔargB, lack possess production, respectively. Deficiency argEF (encoding...
The amount of intracranial blood is a strong predictor poor outcome after subarachnoid hemorrhage (SAH). Here, we aimed to measure iron concentrations in the cerebral white matter, using microdialysis (CMD) technique, and associate levels with local metabolic profile, complications, functional outcome.For observational cohort study, 36 patients consecutive grade SAH (Hunt & Hess 4 or 5, Glasgow Coma Scale Score ≤ 8) undergoing multimodal neuromonitoring were analyzed for brain changes,...
Iron overload can adversely influence the course of infection by increasing microbial replication and suppressing antimicrobial immune effector pathways. Recently, we have shown that calcium channel blocker nifedipine mobilize tissue iron in mouse models overload. We therefore investigated whether treatment affects with intracellular bacteria via modulation homeostasis.The effect on intramacrophage cellular homeostasis was murine macrophage cell line RAW264.7, impact systemic C57BL/6 mice...
During normal spermatogenesis, the testis-specific linker histone H1t appears at pachytene stage becomes phosphorylated in early spermatids and disappears late spermatids. Using reversed-phase hydrophilic interaction liquid chromatography, from rat mouse testes was isolated, subjected to enzymatic digestion, analyzed by mass spectrometry. We observed different states of (mono-, di-, triphosphorylated) as well unphosphorylated protein. Tandem spectrometry immobilized metal ion affinity...