A5090055659- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Spaceflight effects on biology
- Rheumatoid Arthritis Research and Therapies
- Cancer, Hypoxia, and Metabolism
- Wildlife Ecology and Conservation
- Spectroscopy and Chemometric Analyses
- Advanced Chemical Sensor Technologies
- RNA modifications and cancer
- Traditional Chinese Medicine Studies
- Metabolism, Diabetes, and Cancer
- Microscopic Colitis
- Computational Drug Discovery Methods
- Spondyloarthritis Studies and Treatments
- Exercise and Physiological Responses
- Celiac Disease Research and Management
- Asthma and respiratory diseases
- NMR spectroscopy and applications
- Effects of Environmental Stressors on Livestock
- ATP Synthase and ATPases Research
- Analytical Chemistry and Chromatography
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- Cystic Fibrosis Research Advances
- Genetic and phenotypic traits in livestock
Sapienza University of Rome
2012-2025
Università degli Studi della Tuscia
2022
Washington State University
2019-2021
Polytechnic University of Bari
2015
Background Cystic fibrosis (CF) is a disorder affecting the respiratory, digestive, reproductive systems and sweat glands. This lethal hereditary disease has known or suspected links to dysbiosis gut microbiota. High-throughput meta-omics-based approaches may assist in unveiling this complex network of symbiosis modifications. Objectives The aim study was provide predictive functional model microbiota enterophenotype pediatric patients affected by CF under clinical stability. Methods...
Abstract Background Altered metabolism is one of the cancer hallmarks. The role circRNAs in poorly studied. Specifically, impact circPVT1, a well-known oncogenic circRNA on triple negative breast mechanistically underexplored. Methods clinical significance circPVT1 expression levels was assessed human samples using digital PCR and genome atlas (TCGA) dataset. activity TNBC cell lines MCF-10 A line by either ectopic or depletion molecule. CircPVT1 mediated metabolic perturbation 1 H-NMR...
An interlaboratory comparison (ILC) was organized with the aim to set up quality control indicators suitable for multicomponent quantitative analysis by nuclear magnetic resonance (NMR) spectroscopy. A total of 36 NMR data sets (corresponding 1260 spectra) were produced 30 participants using 34 spectrometers. The calibration line method chosen quantification a five-component model mixture. Results show that is robust tool and 26 out resulted in statistically equivalent lines all considered...
// Mario Cioce 1* , MariaCristina Valerio 2* Luca Casadei 2 Claudio Pulito 3 Andrea Sacconi 4 Federica Mori Francesca Biagioni Cesare Manetti Paola Muti 5 Sabrina Strano and Giovanni Blandino . 1 Department of Cardiothoracic Surgery, NYU Langone Medical Center, New York, NY USA Chemistry, University Rome 'La Sapienza', 00185 Rome, Italy Molecular Chemoprevention Group, Italian National Cancer Institute "Regina Elena", Translational Oncogenomic Unit, Oncology, McMaster University, Hamilton,...
// Claudio Pulito 1 , Federica Mori Andrea Sacconi 2 Luca Casadei 3 Maria Ferraiuolo Cristina Valerio Raffaela Santoro Frauke Goeman Anna Maidecchi 4 Luisa Mattoli Cesare Manetti Silvia Di Agostino Paola Muti 5 Giovanni Blandino 2, Sabrina Strano 1, Molecular Chemoprevention Unit, Medicine Area, Regina Elena National Cancer Institute, Rome, Italy Translational Oncogenomic Department of Chemistry, University Rome "La Sapienza", Aboca SpA Società Agricola, Sansepolcro, Oncology, Juravinski...
Objective A considerable proportion of patients with rheumatoid arthritis (RA) do not have a satisfactory response to biological therapies. We investigated the use metabolomics approach identify biomarkers able anticipate biologics in RA patients. Methods Due gender differences metabolomic profiling, analysis was restricted female starting etanercept as first treatment and having minimum six months' follow-up. Each patient evaluated by same rheumatologist before after months treatment. At...
Abstract Many pivotal biological cell processes are affected by gravity. The aim of our study was to evaluate and functional effects, differentiation potential exo-metabolome profile simulated microgravity (SMG) on human hepatic line (HepG2) biliary tree stem/progenitor cells (hBTSCs). Both hBTSCs HepG2 were cultured in a weightless protected environment SGM produced the Rotary Cell Culture System (Synthecon) control condition normal gravity (NG). Self-replication toward mature determined...
Abstract Background and Aims Nonanastomotic biliary strictures (NAS) are a major cause of morbidity after orthotopic liver transplantation (OLT). Although ischemic injury peribiliary glands (PBGs) vascular plexus during OLT has been associated with the later development NAS, exact underlying mechanisms remain unclear. We hypothesized that bile ducts patients NAS suffer from ongoing hypoxia lack regeneration PBG stem/progenitor cells. Approach Results Forty‐two patients, requiring...
Solid tumours are less oxygenated than normal tissues. Consequently, cancer cells acquire to be adapted a hypoxic environment. The poor oxygenation of solid is also major indicator an adverse prognosis and leads resistance conventional anticancer treatments. We previously showed the involvement Che-1/AATF (Che-1) in cell survival under stress conditions. Herein we hypothesized that Che-1 plays role response hypoxia. human colon adenocarcinoma HCT116 HT29 lines undepleted or depleted for...
Over the past decade, newly designed cancer therapies have not significantly improved survival of patients diagnosed with Malignant Pleural Mesothelioma (MPM). Among a limited number genes that are frequently mutated in MPM several them encode proteins belong to HIPPO tumor suppressor pathway. The anticancer effects top flower standardized extract Filipendula vulgaris (Dropwort) were characterized "in vitro" and vivo" models MPM. At molecular level, two "omic" approaches used investigate...
Traditional measures of short-term stress response such as fecal glucocorticoid metabolites (FGM) are widely used in controlled settings to quantify the intensity stimulation which cattle exposed. However, FGMs present several methodological and interpretation pitfalls when applied on animals free-ranging conditions. In this study, we proposed an NMR-based metabolomics strategy for noninvasive detection beef cattle. Using a longitudinal sample collection, monitored changes metabolome FGM...
Abstract Few field tests have assessed the effects of predator-induced stress on prey fitness, particularly in large carnivore-ungulate systems. Because traditional measures present limitations when applied to free-ranging animals, new strategies and systemic methodologies are needed. Recent studies shown that anxiety related behaviors can influence metabolic activity gut microbiome mammal hosts, these alterations may aid identification stress. In this study, we used NMR-based fecal...
Abstract Altered metabolism is one of the cancer hallmarks. The role circRNAs in still unexplored. Herein, we initially found that expression circPVT1 was significantly higher tumoral tissues than non-tumoral breast tissues. Basal like patients with levels exhibited shorter disease-free survival compared to those lower expression. CircPVT1 ectopic rendered fully transformed MCF-10A immortalized cells and increased tumorigenicity TNBC cell lines. Depletion endogenous reduced SUM-159PT...
As wolves recolonize across their former range in western North America, encounters between livestock and are expected to increase frequency. Understanding the physiological state of prey, as a response stress imposed by presence predator (trait-mediated effects), will help with predicting total effect predators on prey beyond direct consumption (density-mediated effects). Fecal glucocorticoid (GCM) is widely used measure response, but provides inconclusive results, particularly when applied...
<h3>Background</h3> Metabolomics belongs to the family of “-<i>omics</i>” sciences, all which share advantage a non-targeted approach for identifying biomarkers and profiling patient. Metabolomic procedure has become feasible recently with advent accessibility new high-throughput technologies, including mass spectrometry <sup>1</sup>H Nuclear Magnetic Resonance (<sup>1</sup>H NMR) few studies have been published in rheumatic disorders. <h3>Objectives</h3> To evaluate whether NMR-based...