A5026312275- Liver physiology and pathology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Pediatric Hepatobiliary Diseases and Treatments
- Drug Transport and Resistance Mechanisms
- 3D Printing in Biomedical Research
- Liver Diseases and Immunity
- Spaceflight effects on biology
- Organ Transplantation Techniques and Outcomes
- Metabolism, Diabetes, and Cancer
- Peptidase Inhibition and Analysis
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Diabetes and associated disorders
- Ubiquitin and proteasome pathways
- Liver Disease Diagnosis and Treatment
- Cancer Cells and Metastasis
- Diabetes Management and Research
- FOXO transcription factor regulation
- Pancreatic and Hepatic Oncology Research
- Cancer-related molecular mechanisms research
- Cell death mechanisms and regulation
- Cancer Diagnosis and Treatment
- Genetics and Neurodevelopmental Disorders
Università Campus Bio-Medico
2025
University of Milan
2020-2024
Sapienza University of Rome
2014-2022
Foro Italico University of Rome
2014
University of Rome Tor Vergata
2014
Cholangiocarcinoma (CCA) is an aggressive cancer with high resistance to chemotherapeutics. CCA enriched in stem cells, which correlate aggressiveness and prognosis. FXR, a member of the metabolic nuclear receptor family, markedly down-regulated human CCA. Our aim was evaluate, primary cultures intrahepatic (iCCA), effects FXR agonist obeticholic acid (OCA), semisynthetic bile derivative, on their cancerogenic potential. Primary iCCA cell were prepared from surgical specimens mucinous or...
Abstract Duchenne muscular dystrophy (DMD) affects 1 in 3500 live male births. To date, there is no effective cure for DMD, and the identification of novel molecular targets involved disease progression important to design more treatments therapies alleviate DMD symptoms. Here, we show that protein levels Bromodomain extra-terminal domain (BET) BRD4 are significantly increased muscle mouse model mdx mouse, pharmacological inhibition BET proteins has a beneficial outcome, tempering oxidative...
Mechanisms underlying the repair of extrahepatic biliary tree (EHBT) after injury have been scarcely explored. The aims this study were to evaluate, by using a lineage tracing approach, contribution peribiliary gland (PBG) niche in regeneration EHBT damage and vivo vitro, signaling pathways involved.Bile duct was induced administration 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) diet for 14 days Krt19Cre TdTomatoLSL mice. Human stem/progenitor cells (BTSC) within PBGs isolated from...
We investigated the sensitivity of intrahepatic cholangiocarcinoma (IHCCA) subtypes to chemotherapeutics and molecular targeted agents. Primary cultures mucin- mixed-IHCCA were prepared from surgical specimens (N. 18 IHCCA patients) evaluated for cell proliferation (MTS assay) apoptosis (Caspase 3) after incubation (72 hours) with increasing concentrations different drugs. In vivo, subcutaneous human tumor xenografts evaluated. characterized by a pattern expression cancer stem markers, drug...
Cholangiocarcinoma (CCA) is a very aggressive cancer showing the presence of high stem cells (CSCs). Doublecortin-like kinase1 (DCLK1) has been demonstrated as CSC marker in different gastroenterological solid tumors. Our aim was to evaluate vitro expression and biological function DCLK1 intrahepatic CCA (iCCA) perihilar (pCCA).Specimens surgically resected human were enzymatically digested, submitted immunosorting for specific markers (LGR5 [leucine-rich repeat-containing G protein-coupled...
Abstract Many pivotal biological cell processes are affected by gravity. The aim of our study was to evaluate and functional effects, differentiation potential exo-metabolome profile simulated microgravity (SMG) on human hepatic line (HepG2) biliary tree stem/progenitor cells (hBTSCs). Both hBTSCs HepG2 were cultured in a weightless protected environment SGM produced the Rotary Cell Culture System (Synthecon) control condition normal gravity (NG). Self-replication toward mature determined...
Objective An aneurysm is a localized dilation of blood vessel, commonly referring to the arterial system, but venous aneurysms, especially in lower limbs, are significant and often underreported vascular conditions. This study focuses on superficial aneurysms at sapheno-femoral junction (SFJ), categorized as Type IA IB. Materials Methods Eleven patients (5 females, 6 males; mean age 50 years) with masses were identified after negative hernia evaluations. Diagnosis was established through...
Cell therapy of liver diseases with human biliary tree stem cells (hBTSCs) is biased by low engraftment efficiency. Coating the hBTSCs hyaluronans (HAs), primary constituents all cell niches, could facilitate survival, proliferation, and, specifically, given that HAs are cleared selectively liver.We developed a fast and easy method to coat HA assessed effects HA-coating on properties in vitro vivo.The coating markedly improved viability, colony formation, population doubling cultures,...
Abstract Intrahepatic cholangiocarcinoma (iCCA) represents a heterogeneous group of malignancies emerging from the biliary tree, often in context chronic bile ducts inflammation. The immunological features iCCA cells and their capability to control lymphocytes response have not yet been investigated. aims present study were evaluate interaction between human peripheral blood mononuclear (PBMCs) role Fas/FasL modulating T-cells NK-cells after direct co-culture. express high levels Fas FasL...
Abstract Intrahepatic cholangiocarcinoma (iCCA) is a highly aggressive cancer with marked resistance to chemotherapeutics without therapies. The tumour microenvironment of iCCA enriched Cancer-Stem-Cells expressing Epithelial-to-Mesenchymal Transition (EMT) traits, being these features associated aggressiveness and drug resistance. Treatment the anti-diabetic Metformin, has been recently reduced incidence iCCA. We aimed evaluate anti-cancerogenic effects Metformin in vitro vivo on primary...
Abstract Peribiliary glands (PBGs) are niches in the biliary tree and containing heterogeneous endodermal stem/progenitors cells that can differentiate, vitro vivo, toward pancreatic islets. The aim of this study was to evaluate, experimental human diabetes, proliferation PBGs differentiation stem/progenitor (BTSCs) insulin-producing cells. Diabetes generated mice by intraperitoneal injection a single dose 200 mg/kg (N = 12) or 120 streptozotocin. Liver, pancreas, extrahepatic trees were en...
Abstract Human biliary tree stem/progenitor cells (hBTSCs) are being used for cell therapies of patients with liver cirrhosis. A cryopreservation method was established to optimize sourcing hBTSCs these clinical programs and that comprises serum-free Kubota’s Medium (KM) supplemented 10% dimethyl sulfoxide (DMSO), 15% human serum albumin (HSA) 0.1% hyaluronans. Cryopreserved versus freshly isolated were similar in vitro respect self-replication, stemness traits, multipotency. They able...
Hepatoma Research is an open access journal and focuses on all topics related to hepatoma. The following articles are especially welcome: pathogenesis, clinical examination early diagnosis of hepatoma, complications their preventions treatments, etc.
Obesity, one of the most frequent health problems in adult population, is a condition characterized by excessive white adipose tissue accumulation and accompanied increased risk to develop other disorders such as type II diabetes, cardiovascular disorders, physical disability, frailty sarcopenia. Total fat mass frequently increases during aging, often coexisting with sarcopenia, thus resulting an emerging defined sarcopenic obesity (SO). Our previous data demonstrated relevant role bromo...
Generation of β-pancreatic cells represents a major goal in research. The aim this study was to explore protein-based strategy induce differentiation human biliary tree stem (hBTSCs) towards cells. A plasmid containing the sequence pancreatic and duodenal homeobox 1 (PDX1) has been expressed E. coli. Epithelial-Cell-Adhesion-Molecule positive hBTSCs or mature hepatocyte cell line, HepG2, were grown medium which Pdx1 peptide added. Differentiation toward islet evaluated by expression β-cell...
Contrasting evidence is present regarding the contribution of stem/progenitor cell populations to pancreatic regeneration in diabetes. Interestingly, a compartment with features has been identified duct glands (PDGs). The aims study were evaluate islet injury and regeneration, participation PDG type 2 diabetic mellitus (T2DM) an experimental model Human pancreata obtained from normal (N = 5) or T2DM 10) cadaveric organ donors. Experimental diabetes was generated mice by intraperitoneal...
Human biliary tree stem/progenitor cells (hBTSCs), reside in peribiliary glands, are mainly stimulated by primary sclerosing cholangitis (PSC) and cholangiocarcinoma. In these pathologies, hBTSCs displayed epithelial-to-mesenchymal transition (EMT), senescence characteristics, impaired differentiation. Here, we investigated the effects of cholest-4,6-dien-3-one, an oxysterol involved cholangiopathies, on biology. were isolated from donor organs, cultured self-renewal control conditions,...
Abstract A major function of the intrahepatic biliary epithelium is bicarbonate excretion in bile. Recent reports indicate that budesonide, a corticosteroid with high receptor affinity and hepatic first pass clearance, increases efficacy ursodeoxycholic acid, choleretic agent, primary cholangitis patients. We have previously reported bile ducts isolated from rats treated dexamethasone or budesonide showed an enhanced activity Na + /H exchanger isoform 1 (NHE1) Cl − /HCO 3 protein 2 (AE2) ....