A5011220231- Sepsis Diagnosis and Treatment
- Immune Response and Inflammation
- Phagocytosis and Immune Regulation
- Advanced Glycation End Products research
- Heart Failure Treatment and Management
- Cytokine Signaling Pathways and Interactions
- Cell death mechanisms and regulation
- Intensive Care Unit Cognitive Disorders
- Cardiac Ischemia and Reperfusion
- Platelet Disorders and Treatments
- Macrophage Migration Inhibitory Factor
- Inflammatory mediators and NSAID effects
- Phosphodiesterase function and regulation
- Immunodeficiency and Autoimmune Disorders
- Mast cells and histamine
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Dietary Effects on Health
- Autophagy in Disease and Therapy
- S100 Proteins and Annexins
- Histone Deacetylase Inhibitors Research
- Pharmacological Effects of Natural Compounds
- FOXO transcription factor regulation
- Gut microbiota and health
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
Universidade Federal de Santa Catarina
2017-2025
Università di Camerino
2024-2025
University of Turin
2020-2024
Universidade Estadual de Ponta Grossa
2017
Heat-processed diets contain high amounts of advanced glycation end products (AGEs). Here we explore the impact an AGE-enriched diet on markers metabolic and inflammatory disorders as well gut microbiota composition plasma proteins glycosylation pattern. C57BL/6 mice were allocated into control (CD, n = 15) (AGE-D, for 22 weeks. AGE-D was prepared replacing casein by methylglyoxal hydroimidazolone-modified casein. evoked increased insulin a significant reduction GIP/GLP-1 incretins ghrelin...
Sepsis is caused by systemic infection and a major health concern as it the primary cause of death from infection. It leading mortality worldwide there are no specific effective treatments for sepsis. Gene deletion neutral solute channel Aquaporin 9 (AQP9) normalizes oxidative stress improves survival in bacterial endotoxin induced mouse model In this study we described initial characterization effects novel small molecule AQP9 inhibitor, RG100204, cecal ligation puncture (CLP) polymicrobial...
Sepsis is a life-threatening condition that affects the cardiovascular and renal systems. Severe hypotension during sepsis compromises tissue perfusion, which can lead to multiple organ dysfunction death. Phosphodiesterase 5 (PDE5) degrades intracellular cyclic guanosine monophosphate (cGMP) levels promotes vasodilatation in specific sites. Our previous studies show inhibiting cGMP production early increases mortality, implying protective role for production. Then, we hypothesized increased...
Recent evidence suggests the substantial pathogenic role of Janus kinase (JAK)/signal transducer and activator transcription (STAT) pathway in development low-grade chronic inflammatory response, known as "metaflammation," which contributes to obesity type 2 diabetes. In this study, we investigated effects JAK1/2 inhibitor baricitinib, recently approved for treatment rheumatoid arthritis, a murine high-fat-high sugar diet model. Male C57BL/6 mice were fed with control normal (ND) or (HD) 22...
Hemorrhagic shock (HS), which causes insufficient tissue perfusion, can result in multiple organ failure (MOF) and death. This study aimed to evaluate whether doxycycline (DOX) protects cardiovascular, kidney, liver from damage a rat model of HS. Immediately before the resuscitation, DOX (10 mg/kg; i.v.) was administered, its protective effects were assessed 24 h later. Mean arterial pressure, renal blood flow, heart rate, vasoactive drug response, markers such as urea, creatinine, AST, ALT,...
We previously reported the Bruton's tyrosine kinase (BTK) inhibitors ibrutinib and acalabrutinib improve outcomes in a mouse model of polymicrobial sepsis. Now we show that genetic deficiency BTK gene alone Xid mice confers protection against cardiac, renal liver injury sepsis reduces hyperimmune stimulation ('cytokine storm') induced by an overwhelming bacterial infection. Protection is due part to enhanced phagocytosis vivo, changes lipid metabolism decreased activation NF-B NLRP3...
Inhibition of either P2Y12 receptor or the nucleotide-binding oligomerization domain- (NOD-) like pyrin domain containing 3 (NLRP3) inflammasome provides cardioprotective effects. Here, we investigate whether direct NLRP3 inhibition exerts additive effects on myocardial protection induced by antagonist Ticagrelor. Ticagrelor (150 mg/kg) was orally administered to rats for three consecutive days. Then, isolated hearts underwent an ischemia/reperfusion (30 min ischemia/60 reperfusion; IR)...
Sepsis and septic shock are associated with high mortality considered one of the major public health concerns. The onset sepsis is known as a hyper-inflammatory state that contributes to organ failure mortality. Recent findings suggest potential role two non-receptor protein tyrosine kinases, namely Focal adhesion kinase (FAK) Proline-rich 2 (Pyk2), in inflammation endometriosis, cancer, atherosclerosis asthma. Here we investigate FAK-Pyk2 pathogenesis beneficial effects pharmacological...
The aim of this study was to investigate (a) the effects Janus kinase (JAK)/signal transducer and activator transcription (STAT) pathway inhibitor (baricitinib) on multiple organ dysfunction syndrome (MODS) in a rat model hemorrhagic shock (HS) (b) whether treatment with baricitinib attenuates activation JAK/STAT, NF-κB, NLRP3 caused by HS.Posttraumatic MODS, which is part due excessive systemic inflammation, associated high morbidity mortality. JAK/STAT regulator numerous growth factor...
Sepsis is one of the major complications surgery resulting in high morbidity and mortality, but there are no specific therapies for sepsis-induced organ dysfunction. Data obtained under Gene Expression Omnibus accession GSE131761 were re-analyzed showed an increased gene expression Janus Kinase 2 (JAK2) Signal Transducer Activator Transcription 3 (STAT3) whole blood post-operative septic patients. Based on these results, we hypothesized that JAK/STAT activation may contribute to...
Obesity, one of the most frequent health problems in adult population, is a condition characterized by excessive white adipose tissue accumulation and accompanied increased risk to develop other disorders such as type II diabetes, cardiovascular disorders, physical disability, frailty sarcopenia. Total fat mass frequently increases during aging, often coexisting with sarcopenia, thus resulting an emerging defined sarcopenic obesity (SO). Our previous data demonstrated relevant role bromo...
Advanced glycation end products (AGEs) exert a key pathogenic role in the development of obesity and insulin resistance. Thanks to its abundance bioactive compounds, microalga Arthrospira platensis (spirulina, SP) is proposed as nutritional supplement. Here, we investigated potential anti-glycating properties SP enriched with zinc (Zn-SP) following impact on diet-induced metabolic derangements. Thirty male C57Bl6 mice were fed standard diet (SD) or high-fat high-sugar (HFHS) for 12 weeks,...
KRIT1 loss-of-function mutations underlie the pathogenesis of Cerebral Cavernous Malformation (CCM), a major vascular disease affecting central nervous system (CNS). However, is also expressed outside CNS and modulates key regulators metabolic oxy-inflammatory pathways, including master transcription factor FoxO1, suggesting widespread functional significance. Herein, we show that KRIT1/FoxO1 axis implicated in liver functions antioxidative/antiglycative defenses. Indeed, by performing...
Septic shock, which is triggered by microbial products, mainly characterised inadequate tissue perfusion, can lead to multiple organ dysfunction and death. An intense release of vasoconstrictors agents occurs in the early stages ischemic injury. In this scenario, cGMP could play a key role counterbalancing these preventing damage. Sildenafil, phosphodiesterase-5 inhibitor, increases smooth muscle cells promotes vasodilation. Thus, purpose study was investigate effect treatment with...
The aim of this study was to investigate (a) the potential Bruton's tyrosine kinase (BTK) inhibitors acalabrutinib and fenebrutinib reduce multiple organ dysfunction syndrome (MODS) in acute (short-term long-term follow-up) hemorrhagic shock (HS) rat models (b) whether treatment with either or attenuates BTK, NF-κB NLRP3 activation HS.The MODS caused by an excessive systemic inflammatory response following trauma is associated a high morbidity mortality. protein BTK known play role...
The aim of this study was to investigate (a) macrophage migration inhibitory factor (MIF) levels in polytrauma patients and rats after haemorrhagic shock (HS), (b) the potential MIF inhibitor ISO-1 reduce multiple organ dysfunction syndrome (MODS) acute (short-term long-term follow-up) HS rat models (c) whether treatment with attenuates NF-κB NLRP3 activation HS. MODS caused by an excessive systemic inflammatory response following trauma is associated a high morbidity mortality. pleiotropic...
Casein kinase II (CK2) has recently emerged as a pivotal mediator in the propagation of inflammation across various diseases. Nevertheless, its role pathogenesis sepsis remains unexplored. Here, we investigated involvement CK2 progression and potential beneficial effects silmitasertib, selective potent CK2α inhibitor, currently under clinical trials for COVID-19 cancer. Sepsis was induced by caecal ligation puncture (CLP) four-month-old C57BL/6OlaHsd mice. One hour after CLP/Sham procedure,...
Inducible T cell co-stimulator (ICOS), an immune checkpoint protein expressed on activated cells and its unique ligand, ICOSL, which is antigen-presenting non-hematopoietic cells, have been extensively investigated in the response. Recent findings showed that a soluble recombinant form of ICOS (ICOS-Fc) can act as innovative immunomodulatory drug both antagonist agonist modulating cytokine release migration to inflamed tissues. Although ICOS-ICOSL pathway has poorly septic context, few...
Periodontitis is associated with endothelial dysfunction, which clinically characterized by a reduction in endothelium-dependent relaxation. However, we have previously shown that impairment relaxation transient. Therefore, evaluated mediators are involved recovery.Rats were subjected to ligature-induced experimental periodontitis. Twenty-one days after the procedure, animals prepared for blood pressure recording, and responses acetylcholine or sodium nitroprusside obtained before 30 minutes...
Candesartan cilexetil (CC) is a poorly soluble antihypertensive drug with in vivo absorption limited by its low aqueous solubility. Aiming to generate CC supersaturation as strategy improve and bioavailability, amorphous solid dispersions (ASDs) of hydroxypropylmethylcellulose acetate succinate type M (HPMCAS M) were developed evaluated vitro techniques. The ASDs characterized several solid-state techniques regarding the generation maintenance under non-sink conditions biorelevant medium....
Metabolic endotoxemia has been suggested to play a role in the pathophysiology of metaflammation, insulin-resistance and ultimately type-2 diabetes mellitus (T2DM). The endogenous antimicrobial peptides (AMPs), such as cathelicidin LL-37, T2DM is unknown. We report here for first time that patients with compared healthy volunteers have elevated plasma levels LL-37. In reverse-translational approach, we investigated effects AMP, peptide 19-2.5, murine model high-fat diet (HFD)-induced...