A5005886154- Nitric Oxide and Endothelin Effects
- Sepsis Diagnosis and Treatment
- Eicosanoids and Hypertension Pharmacology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Receptor Mechanisms and Signaling
- Cardiac Ischemia and Reperfusion
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Renin-Angiotensin System Studies
- Immune Response and Inflammation
- Neuropeptides and Animal Physiology
- Neuroendocrine regulation and behavior
- Ion channel regulation and function
- Intensive Care Unit Cognitive Disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Trypanosoma species research and implications
- Stress Responses and Cortisol
- Research on Leishmaniasis Studies
- S100 Proteins and Annexins
- Neuroscience and Neuropharmacology Research
- Natural product bioactivities and synthesis
- Respiratory Support and Mechanisms
- Hormonal Regulation and Hypertension
- Drug Solubulity and Delivery Systems
- Estrogen and related hormone effects
- Pharmacological Effects of Natural Compounds
Universidade Federal de Santa Catarina
2015-2024
Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina
2024
National Council for Scientific and Technological Development
1993-2024
Fundação de Amparo à Pesquisa do Espírito Santo
2024
Coordenação de Aperfeicoamento de Pessoal de Nível Superior
2024
University of Turin
2015
William Harvey Research Institute
2015
Queen Mary University of London
2015
Polydoro Ernani de São Thiago University Hospital
2002-2013
Universidade Federal de Minas Gerais
2005
1 A murine macrophage cell line, J774, expressed nitric oxide (NO) synthase activity in response to interferon-gamma (IFN-γ, 10 u ml−1) plus lipopolysaccharide (LPS, ng ml−1). The enzyme was first detectable 6 h after incubation, peaked at 12 and became undetectable 48 h. 2 decline the NO not due inhibition by stable substances secreted cells into culture supernatant. 3 significantly slowed down cultured a low l-arginine medium or with added haemoglobin, suggesting that may be involved...
Abstract Murine macrophages can be activated to produce nitric oxide (NO) and superoxide these two radicals react form peroxynitrite, a powerful oxidant which may involved in parasite killing. We now show that murine with zymosan interferon‐γ (ZYM/IFN‐γ) produced both (peaking 1–2 h after stimulation, then rapidly declining) NO (barely detectable at 6 h, peaking by 24 h). Macrophages ZYM alone only superoxide, while stimulation lipopolysaccharide (LPS) IFN‐γ induced but not superoxide. Cells...
<!?tlsb=.005w>Caffeic acid and some of its derivatives such as caffeic phenetyl ester (CAPE) octyl caffeate are potent antioxidants which present important anti-inflammatory actions. The study assessed the in vitro vivo effects five (caffeic methyl, ethyl, butyl, benzyl esters) compared their actions to those CAPE. In model LPS-induced nitric oxide (NO) production RAW 264.7 macrophages, pre-incubation all inhibited nitrite accumulation on supernatant stimulated cells, with mean IC50 (μM)...
Septic shock is a dangerous condition with high mortality rates. In sepsis, the inducible form of nitric oxide (NO) synthase induced, releasing amounts NO. Glucocorticoids have potent anti-inflammatory properties and are very effective in inhibiting induction this enzyme if administered before onset. It known that glucocorticoid receptor (GR) has critical cysteine residues for steroid binding its hormone-binding DNA-binding domains. also been reported NO reacts —SH groups,...
Rationale: Recovering the neutrophil migration to infectious focus improves survival in severe sepsis. Recently, we demonstrated that cystathionine γ-lyase (CSE)/hydrogen sulfide (H2S) pathway increased recruitment inflammatory during sterile inflammation.Objectives: To evaluate if H2S administration increases and of mice.Methods: Sepsis was induced by cecal ligation puncture (CLP).Measurements Main Results: The pretreatments mice with donors (NaHS or Lawesson's reagent) improved leukocyte...
This study aimed to evaluate the systemic inflammatory response and cardiovascular changes induced by experimental periodontitis in rats. Experimental was placing a cotton ligature around cervix of both sides mandibular first molars maxillary second each male rat. Sham-operated rats had removed immediately after procedure. Seven, 14 or 28 days procedure, effects acetylcholine, sodium nitroprusside phenylephrine were evaluated on blood pressure, aortic rings isolated perfused mesenteric bed....
Abstract Sepsis is one of the leading causes acute kidney injury (AKI), and several mechanisms including microcirculatory alterations, oxidative stress, endothelial cell dysfunction are involved. Nitric oxide (NO) common elements to all these mechanisms. Although three nitric synthase (NOS) isoforms constitutively expressed within kidneys, they contribute in different ways nitrergic signaling. While (eNOS) neuronal (nNOS) likely be main sources NO under basal conditions participate...
Ten chalcones were synthesized and tested as potential leishmanicidal trypanocidal agents. All compounds caused concentration-dependent inhibition of the in vitro growth Leishmania braziliensis Trypanosoma cruzi with no significant toxic effect towards host macrophages. Our results show that positions substituents seem to be critical for their antiprotozoal activities.
Abstract: In chronically inflamed animals, adrenal hormones exert a positive control on the secretion of melatonin by pineal gland. this paper, mechanism corticosterone as modulator and N ‐acetylserotonin (NAS) was determined. Rat glands in culture, stimulated for 5 hr with noradrenaline (10 n m ), were previously incubated (1.0 –1.0 μ ) 48 presence or absence glucocorticoid receptor (GR) antagonist, mifepristone proteasome inhibitor, ‐acetyl‐leucinyl‐leucinyl‐norleucinal‐H (ALLN, 12.5...
Nanosponges are a novel class of hyperbranched cyclodextrin-based nanostructures that exhibits remarkable potential as drug host system for the improvement in biopharmaceutical properties. This work aims development nanosponge norfloxacin to improve its physicochemical characteristics. β-cyclodextrin was used base and diphenyl carbonate crosslinker agent at different proportions produce nanosponges were evaluated by vitro vivo techniques. The proportion cyclodextrin:crosslinker 1:2 M/M...
Nitric oxide (NO*) is synthesized in skeletal muscle and its production increases during contractile activity. Although myosin the most abundant protein muscle, it not known whether a target of NO* or derivatives. In present study, we have shown that exercise S-nitrosylation and, among proteins, principal exogenous SNOs (S-nitrosothiols) both skinned fibres differentiated myotubes. The reaction isolated with S-nitrosoglutathione results at multiple cysteine thiols produces two populations...
Recently, there has been much attention paid to understanding the molecular mechanisms underlying apoptosis and functional consequences of apoptotic body clearance by phagocytes. In an attempt investigate this latter aspect, present study evaluated anti-inflammatory effects in vivo administration phosphatidylserine (PS) liposomes, a well-characterised membrane component expressed during apoptosis. The participation peroxisome proliferator-activated receptors (PPARs) PS-mediated was also...
In the present study, we investigated role of noradrenergic transmission in unconditioned and conditioned responses to predatory threats. First, examined effects systemically injected beta-blockers on contextual response cat odor. The centrally acting beta-blocker (propranolol) was able impair responses, as well acquisition fear odor; however, peripherally (nadolol) not effective. Next, neural substrate underlying modulation defensive odor focused dorsal premammillary nucleus (PMd), because...
It has been reported that stress-related activation of the noradrenergic system strengthens formation aversive memories and beta-adrenergic receptors seem to be involved in this emotional memory processing. In study, effects compounds on extinction contextual conditioned fear responses were evaluated. Rats trained with footshock a conditioning box. 3 days following training, animals re-exposed apparatus received either single or repeated intraperitoneal injections antagonist propranolol,...
Lipoxin A4 (LXA4) is an endogenous lipid mediator with potent anti-inflammatory actions but its role in infectious processes not well understood. We investigated the involvement of LXA4 and receptor FPR2/ALX septic inflammatory dysregulation. Pneumosepsis was induced mice by inoculation Klebsiella pneumoniae. levels expression focus as effects treatment agonists (LXA4 BML-111) antagonists (BOC-2 WRW(4)) early (1 h) late (24 sepsis were studied. Sepsis increase LXA4, lung expression, local...
Sepsis is an amplified systemic immune-inflammatory response produced by a microorganism, which involves activation of inflammatory cytokine signaling pathways and oxidative stress. A variety studies have shown that hydralazine (HDZ) has potent antioxidant anti-inflammatory proprieties. Therefore, we hypothesize HDZ can improve the clinical outcome sepsis. Thus, this work aimed to evaluate therapeutic value in reducing response, stress, mortality animal sepsis, investigate its possible...
Neutrophil activation and migration during an inflammatory response is preceded or accompanied by plasma membrane electrical changes. Besides changes in calcium currents, neutrophils have a high permeability to potassium, mainly through potassium channels. However, the significance of channels neutrophil physiology still unclear. Here, we show that treatment rats with ATP-sensitive channel blocker glibenclamide (4, 20, 40 μmol/kg) dose dependently decreased...